Acrosomal integrity of buffalo spermatozoa and its relationship to fertility

Abstract

Acrosomal integrity of neat semen often Murrah buffalo bulls was evaluated by giemsa staining. Eight
bulls had >90 per cent spennatozoa with intact acrosomes, while in two bulls the; intact acrosomes averaged 67.9
and 79.8 per cent. In these two bulls many ejaculates showed a high incidence of detached acrosome; mean values
being 16.9 and 18.8 percent as against2.4 to 7.4 per cent in the remaining bulls. Two consecutive weekly ejaculates
from a bull with a very low and high incidence of detached acrosome were frozen and used in field Al trial. The
conception rate declined by 11.2 per cent when incidence of detached acrosome increased from 3.4 to 26.0 per cent.
These results indicated that among various measurements of ejaculate quality, acrosomal evaluation would help
to identify bulls with potentially lowered fertility.