Assessment of sheep oocyte quality for in vitro maturation

Authors

  • S RANGASAMY Assisted Reproductive Technology Laboratory, Department of Animal Reproduction, Gynaecology and Obstetrics, Madras Veterinary College, Chennai- 600 007
  • K KULASEKAR
  • S BALASUBRAMANIAN
  • G DHINAKAR RAJ

Keywords:

Sheep, Oocytes, In vitro maturation

Abstract

Sheep ovaries were collected from slaughter house and transported to the laboratory within 30 min. A
total of 2117 oocytes were retrieved using slicing technique with a rec~very rate of 4.9 l oocytes per ovary.
Among the collected oocytes 33.87 (717), 32.73 (693) and 33.40 (707) per cent of oocytes were graded as A, B
and C, respectively. The mean maturation percentage of A, Band C grade oocytes in the present study was 95.48
± 0.63, 89.14 ± 1.17 and 77.94 ± 1.12, respectively. The maturation rate of A grade oocytes were significantly
(<0.01) higher than that ofB and C grades and B grade oocytes were significantly (<0.01) higher than that ofC grade oocytes. The overall maturation rate of oocytes in the present study was ~7.5 ± 1.08 per cent.

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Published

2023-02-02

Issue

Vol. 29 No. 1 (2008): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

RANGASAMY, S., KULASEKAR, K., BALASUBRAMANIAN, S., & DHINAKAR RAJ, G. (2023). Assessment of sheep oocyte quality for in vitro maturation. The Indian Journal of Animal Reproduction, 29(1), 55–57. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/5399