THERAPEUTIC MANAGEMENT AND CHANGES IN THE ENDOMETRIUM OF REPEAT BREEDING COWS

Abstract

The overall incidence of repeat breeding observed among 98 breedable Holestein Friesian (HF)
animals of a farm was 40.82 % (n=40). These 40 repeat breeding cows were divided at random In
different groups with a view to evaluate the relative efficacies of different hormonal therapies at breeding
towards improving their reproductive efficiency over the control. The conception rate following i/m treatment
at the time of insemination with GnRH (Receptal, Busere/ln acetate, 0.02 mg), LH (Chorulon, 1500 IU)
and Progesterone (Proluton depot, 500 mg) was 66.66, 83.33 and 50.00 %, respectively, with an overall
mean of 66.66 % (12/18). This was significantly higher (P<0.01) than that (33.33%, 2/6) obtained in
control untreated repeat breeding cows. The overall conception rate in the treatment cycle itself was
significantly (P<0.01 ) higher than in untreated control group (55.55 vs 16.66%). Further, out of 10
chronic repeat breeding cows treated ':Vith PGF 2a (Vetmate, 2 ml) at mid-cycle, 9 (90%) cows came in
estrus within 80 hrs of treatment and 33.33 % (n=3) conceived at first estrus following GnRH treatment
(0.01 mg i/m) at second insemination 12 hrs apart. Out of 18 endometrial biopsy samples collected
from right and left horns and body of the uterus of 6 culled repeat breeding cows, chronic endometritis
was detected in 12 samples (4 animals) and sub-acute endometritis in 6 samples (2 animals). No
significant difference could be observed in histopathological changes pertaining to endometrial biopsies
from left and right uterine horns and uterine body. Although almost identical changes were seen at all
the 3 sites of the uterus, however, microscopic. lesions between animals differed. The endometrial
biopsy proved a good guide to decide the prognosis of chronic repeat breeding animals and GnRH or LH
can be recommended along with A! to improve fertility in infertile cows.