Caffeine in Tris Egg Yolk Citrate Extender Improves the Antioxidant Potential of Cryopreserved Surti Buck Semen

Authors

  • Darshita Vijaybhai Bhut Department of Veterinary Gynaecolog College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari-396 450, Gujarat, Indiay and Obstetrics
  • Chandubhai Talshibhai Khasatiya Department of Veterinary GynaecologCollege of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari-396 450, Gujarat, Indiay and Obstetrics
  • Virendra Kumar Singh 2 Department of Veterinary Physiology and Biochemistry College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari-396 450, Gujarat, India

DOI:

https://doi.org/10.48165/ijar.2024.45.01.3

Keywords:

Antioxidant, Caffeine, Cryopreserved Semen, Glutathione, Malondialdehyde, Motility Degeneration Rate, Surti Buck

Abstract

The study was conducted to evaluate the antioxidant action of caffeine at various concentrations in TEYC extenders.  In the present study, a total 64 ejaculates (16/buck) were collected from all four Surti bucks (age>12 months) i.e. two  collections/week for 8 weeks. Ejaculates at each collection were pooled and divided into four groups viz. C1 (Control),  C2, C3 and C4 having caffeine concentrations @ 0, 1, 3 and 5mM in TEYC extender, respectively with 100x106 sperm/ 

  1. Oxidative stress marker for lipid peroxidation (Malondialdehyde, MDA) and antioxidant (Glutathione, GSH) were  determined at initial, pre-freeze and post-thaw; whereas, motility degeneration rate (MDR) of sperms was evaluated  at pre-freeze and post-thaw. A decrease in GSH and an increase in MDA levels with elapse of time were found to be  significant (p<0.01) between different phases. The MDA levels were observed to be significantly (p<0.01) different  between groups that were highest in C1 and lowest in C4 in contrast to GSH levels that were highest in C4 and lowest in  C1 groups. MDR was significantly (p<0.01) higher at post-thaw than pre-freeze in all the groups. Moreover, MDR was  significantly (p<0.01) lower in C2 group as compared with other groups at both the pre-freeze and post-thaw stage. It was  therefore concluded that for cryopreservation of Surti buck semen, supplementation of caffeine in TEYC extender has  dose-dependent increase in antioxidant defense and decrease in oxidative stress as indicated by higher GSH and lower  lipid peroxidation, respectively but 1mM caffeine concentration in TEYC is optimum and better owing to the lowest  MDR.  

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Published

2024-04-30

Issue

Vol. 45 No. 1 (2024): The Indian Journal of Animal Reproduction( june)

Section

Research Article

How to Cite

Bhut, D.V., Khasatiya, C.T., & Singh, V.K. (2024). Caffeine in Tris Egg Yolk Citrate Extender Improves the Antioxidant Potential of Cryopreserved Surti Buck Semen. The Indian Journal of Animal Reproduction, 45(1), 10–15. https://doi.org/10.48165/ijar.2024.45.01.3
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