Luteinizing Hormone Release in Kisspeptin treated Pre- pubertal Murrah Buffaloes

Authors

  • Himanshu Pandey Animal Reproduction Gynaecology & Obstetrics,
  • Nishant Kumar Animal Reproduction Gynaecology & Obstetrics,
  • Dheeraj Kumar Animal Physiology Division

DOI:

https://doi.org/10.48165/ijar.2024.45.01.1

Keywords:

Buffalo, Kisspeptin, LH, Pre-pubertal

Abstract

Kisspeptin is a neuropeptide that governs the reproductive axis upstream to GnRH. Present study was undertaken to
compare the effect of different doses of kisspeptin on release of luteinizing hormone (LH) in Murrah buffaloes during
pre-pubertal period. Twenty four pre-pubertal buffalo heifers were selected and divided into 4 groups (Control, T1, T2
and T3), having 6 animals in each group. Kisspeptin-10 was administered at the dose of 2 μg/kg body weight, 1.5 μg/
kg body weight, 1μg/kg body weight respectively in T1, T2 and T3 group along with administration of equal volume of
NSS in control group. Blood sampling was done at every 20 minutes interval beginning with zero minutes up to 2 hours
and analyzed for LH concentration. Results indicated that LH concentration was significantly (p<0.05) higher in T1
group. The peak level of LH was recorded at 20 minutes post administration and falls to basal level at 60 minutes post
administration. Although increased LH concentration was observed in other treatment groups but it was lower than T1
group. No LH peak or rise in LH level was observed in control group. The result of this study indicates that during the
pre-pubertal period, administration of Kisspeptin causes dose dependent increase in LH concentration and peak level
was seen at 2 μg/kg body weight of dose.

References

Albers-Wolthers, K.H., De Gier, J., Kooistra, H.S., Rutten, V.P, Van Kooten, P.J., De Graaf, J.J, Leegwater, P.A., Millar, R.P. and Schaefers-Okkens, A.C. (2014). Identification of a novel kisspeptin with high gonadotrophin stimulatory activity in the dog. Neuroendocrinology, 99(3-4):178-189.

Annual report (1999-2000). Buffalo performance at CIRB (Central Institute for research on Buffalo). pp-14. Caraty, A. and Franceschini, I. 2008. Basic aspects of the control of GnRH and LH secretions by kisspeptin: potential appli cations for better control of fertility in females. Reprod. Domest. Anim., 43:172-8.

Chaikhun-Marcou, T., Sotthibandhu, P., Yanprapasiri, C., Pavasutthipaisit, S. and Suadsong, S. (2019). Kiss1 mRNA and Its Protein Distribution in Preoptic and Arcuate Hypothalamic Nuclei in Pre-Pubertal Female Swamp Buffaloes. Pakistan Vet. J., 38: 137-142.

Kadokawa, H., Matsui, M., Hayashi, K., Matsunaga, N., Kawashima, C. and Shimizu, T. (2008). Peripheral admin istration of kisspeptin-10 increases plasma concentrations of GH as well as LH in prepubertal Holstein heifers. J. Endocrinol., 196:331-4

Kinder K, Szczęsna M, Molik E, Misztal ., Zięba DA. (1995). Induction of the secretion of LH and GH by orexin A and ghrelin is controlled in vivo by leptin and photoperiod in sheep, Ann. Anim. Sci., 17 (1), 1-26.

Kotani, M., Detheux, M., Vandenbogaerde, A., Communi, D., Vanderwinden, J.M., Le, P.E., Brezillon, S., Tyldesley, R., Suarez-Huerta, N., Vandeput, F. (2001). The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. J. Biol Chem., 276: 34631–34636.

Lents, C.A., Heidorn, N.L., Barb, C.R. and Ford, J.J. (2008). Central and peripheral administration of kisspeptin acti vates gonadotropin but not somatotropin secretion in pre pubertal gilts. Reproduction. 135(6):879–87.

Leonardi, C.E.P., Dias, F.C.F., Adams, G.P. and Singh, J. (2018). Effect of Kisspeptin-10 on plasma luteinizing hormone concentrations and follicular dynamics during the luteal phase in cattle, Theriogenology, 119: 268-274.

Madan, M.L. (1988). Status of reproduction in female buffalo. In: Buffalo Production and Health: a compendium of latest research information based on Indian studies. ICAR Publication, New Delhi, India, pp. 89–100.

Naniwa, Y., Nakatsukasa, K., Setsuda, S., Oishi, S., Fujii, N. and Matsuda, F. (2013). Effects of full length kisspeptin admin istration on follicular development in Japanese Black beef cows. J. Reprod. Dev., 59: 588-94.

Navarro, V.M., Castellano, J.M., Fernandez-Fernandez, R., Tovar, S., Roa, J. and Mayen, A. 2005.Characterization of the potent luteinizing hormone-releasing activity of KiSS-1 peptide, the natural ligand of GPR54. Endocrinology, 146:156-163.

Pottapenjera, V., Rajanala, S.R., Reddy, C., Gangineni, A., Avula, K., Bejjanki, S.K., Sathagopam, S., Kesharwani, S. and Velmurugan, S. (2018). Kisspeptin modulates luteinizing hormone release and ovarian follicular dynamics in pre-pu

bertal and adult Murrah buffaloes. Front. Vet. Sci., 5:149. Richard, N., Galmiche, G., Corvaisier, S., Caraty, A. and Kottler, M.L. (2008). KiSS-1 and GPR54 genes are co-expressed in rat gonadotrophs and differentially regulated in vivo

The Indian Journal of Animal Reproduction, 45(1): 1-5, June 2024 4

Pandey et al. Luteinizing Hormone Release in Kisspeptin …………………………. Murrah Buffaloes

by oestradiol and gonadotrophin-releasing hormone. J. Neuroendocrinol., 20 381–393.

Terzano, G.M., Barile, V.L. and Borghese, A. (2012). Overview of reproductive endocrine aspects in buffalo. J. Buffalo Sci.,1:126–138.

Wang, J., Sun, L., Zhang, T., Zhou, H. and Lou, Y. (2012). Effect of peripheral administration ofkisspeptin-10 on dynamic LH secretion in prepubertal ewes. Asian-Australasian J. Anim. Sci., 25(6):785.

Whitlock, B.K., Daniel, J.A., Wilborn, R.R., Maxwell, H.S., Steele, B.P. and Sartin, J.L. (2011). Effect of Kisspeptin on Regulation of Growth Hormone. Animal Science Publications and Other Works; https://trace.tennessee.edu/

utk_animpubs/4

Wilborn, R. (2008). Effect of Kisspeptin on the Hypothalamic Pituitary-Gonadal Axis of the Mare. Doctoral dissertation submitted to the Graduate Faculty of Auburn University, USA.

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Published

2024-04-30

Issue

Vol. 45 No. 1 (2024): The Indian Journal of Animal Reproduction( june)

Section

Research Article

How to Cite

Pandey, H., Nishant Kumar, & Kumar, D. (2024). Luteinizing Hormone Release in Kisspeptin treated Pre- pubertal Murrah Buffaloes. The Indian Journal of Animal Reproduction, 45(1), 1–5. https://doi.org/10.48165/ijar.2024.45.01.1
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