Comparative Study of Antibacterial Activity of Tulsi, Garlic  and Commercially used Antibiotics against Bovine Bacterial  Endometritis using Molecular Docking Analysis

Authors

  • Damini Arya Department of Veterinary Gynaecology and Obstetrics G. B. Pant University of Agri. and Tech., Pantnagar, US Nagar, Uttarakhand, India.
  • Rashmi Tyagi Gnit college of Pharmacy Knowledge, Greater Noida, Uttar Pradesh
  • Shingini Sharma CCS National Institute of Animal Health, Baghpat, Uttar Pradesh
  • Mridula Sharma Department of Veterinary Gynaecology and Obstetrics G. B. Pant University of Agri. and Tech., Pantnagar, US Nagar, Uttarakhand, India.

DOI:

https://doi.org/10.48165/ijar.2023.44.02.13

Keywords:

molecular docking, endometritis, garlic, tulsi

Abstract

The present study was conducted with the objective to find effective drug against bacterial bovine endometritis using Molecular docking. The antibacterial property of selected plants was studied and its comparison with conventional antibiotics was done. The plants selected for study were Tulsi and Garlic on the basis of its potent antibacterial activity. Selection of bacteria (S. aureus and E. coli) for the present study was done on the basis of most predominant bacteria causing bovine endometritis as observed in previous studies. The selected bacterial proteins were docked against phytochemicals of plants. Vital proteins of Staphylococcus aureus are Gyrase B (3G75), FtsA (3WQU), DNA ligase (3JSN), Dehydrosqualene synthase (3TFN) and Escherichia coli are FtsZ (6UMK), DNA gyrase (1KZN) and ZapD (5DKO). The docking procedure was carried out using Autodock. It was concluded that,  gentamicin (-6.95 Kcal/mol), enrofloxacin (-6.97 Kcal/mol) and Tulsi (-6.40 Kcal/mol) showed better binding affinity followed by garlic (-3.89 Kcal/mol).  Apigenin, one of the phytoconstituent of tulsi showed the better binding affinity among all the phytochemicals (ranging from -7.2 Kcal/mol to -9.2 Kcal/mol) of garlic and tulsi against targeted bacterial proteins, it can be taken into consideration alone after extraction or in combination therapy. Hence, Tulsi alone or in combination with garlic can be used for further in vivo or in vitro trials in a direction of effective drug discovery against bacterial bovine endometritis.

References

Arunachalam, K. (1980). Antimicrobial activity of Garlic, Onion and Honey. Geobias 7: 46-47.

Batiha, G.E., Beshbishy, A.M., Wasef, L.G., Elewa, Y.H.A., Al-Sagan, A.A., Hack, A.E., Taha, A.E., Abd-Elhakim,

Bicalho, M.L. (2012). Machado VS, Oikonomou G, Gilbert RO, Bicalho RC. Association between virulence factors of Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes and uterine diseases of dairy cows. Vet. Microbiol., 157: 125-131.

Y.M. and Devkota, H.P. (2020).Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review. Nutrients, 12(3): 872-875.

Bicalho, M.L. (2012). Machado VS, Oikonomou G, Gilbert RO, Bicalho RC. Association between virulence factors of Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes and uterine diseases of dairy cows. Vet. Microbiol., 157: 125-131.

Bimmy. (2019). Isolation and identification of predominant bacteria from genital tract of repeat breeder crossbred cows suffering from subclinical endometritis. MVSc thesis G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India.

Deori, S. and Arundhati, P. (2015). Bovine postpartum metritis and its therapeutics: A review. Indian J. of Sci. and Tech., 8(23): 45.

Gupta, A.G. and Deopurkar, R.L. (1993). Microbiological study of Gynecological infection in cattle. Indian J. Anim. Reprod., 14: 118-119.

Kulkarni, K.V. and Adavirao, B.V. (2018). A review on: Indian traditional shrub Tulsi (Ocimum sanctum): the unique medicinal plant. J. Med. Plants Stud., 6(2): 106-110.

Patel, P.P., Panchal, M.T., Kalyani, I.H., Kavani, F.S. (2009). Antibiotic sensitivity spectrum of bacterial isolates from cervico vaginal mucus of postpartum rural buffaloes. Intas Polivet. 10(1): 29-31.

Pillai, V.V. (2012). Efficacy of simultaneous use of enrofloxa cin and metronidazole in the treatment of endometritis in crossbred cattle - a field trial. JIVA, 10(2): 37-39.

Purohit, G.N. (2008). Recent development in the diagnosis and therapy of repeat breeding cows and buffalo. CAB Rev. Pers. Agric. Vet. Sci. Nutr. Nat. Res., 3: 1-34.

Sharma, M. (2017). Comparative Evaluation of Treatment Regimens for Management of Post-partum Endometritis in Crossbred cattle. Intas Polivet.18(1): 29-30.

Sheldon, I.M., Lewis, G.S., Le Blanc, S. and Gilbert, R.O. (2006). Defining postpartum uterine disease in cattle. Theriogenology, 65: 1516-1530.

Sidhu, K.S., Bhangu, S.K, Pathak, R.K, Yadav, I.S, Chhuneja, P. (2020). Identifcation of natural lead compounds for leaf

rust of Wheat: a molecular docking and simulation study. J. Protein Proteomics.11: 283-295.

Singh, S. 2016. Phytotherapeutic measures for endometri tis in crossbred cows. M.V.Sc. thesis submitted to Nanaji Deshmukh Veterinary Science University, Jabalpur.

Sunil. (2008). Use of garlic and Tulsi for the treatment of endo metritis in repeat breeding crossbred cows. MVSc thesis sub mitted to G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India.

Tyagi, M., Tyagi, N. and Tyagi, J. (2021). A Review On: The major constituents of Tulsi (Ocimum sanctum) and their antimicrobial and antioxidant activities against differ ent microbes. Int. Res. J. Mod. Eng. Technol. Sci., 3(6): 370-378.

Downloads

Published

2023-12-29

Issue

Vol. 44 No. 2 (2023): The Indian Journal of Animal Reproduction (Dec) 2023

Section

Research Article

How to Cite

Arya, D., Tyagi, .R., Sharma, S., & Sharma, M. (2023). Comparative Study of Antibacterial Activity of Tulsi, Garlic  and Commercially used Antibiotics against Bovine Bacterial  Endometritis using Molecular Docking Analysis. The Indian Journal of Animal Reproduction, 44(2), 69–74. https://doi.org/10.48165/ijar.2023.44.02.13
Crossref
Scopus
Google Scholar
Europe PMC