Effect of Adenosine 5´ Triphosphate (ATP) and Incubation Time on Frozen Thawed Buffalo Bull Spermatozoa

Authors

  • Misbah Hanif Department of Molecular Biology, Faculty of Science and Technology, Virtual University, Lahore, Pakistan
  • Ejaz Ahmad Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
  • Zahid Naseer Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
  • Muhammad Saleem Akhtar Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
  • Tanveer Ahmad Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
  • Tanveer Hussain Department of Molecular Biology, Faculty of Science and Technology, Virtual University, Lahore, Pakistan
  • Saeed Murtaza Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan

DOI:

https://doi.org/10.48165/ijar.2023.44.02.12

Keywords:

Buffalo bull, ATP, Sperm, Post-thaw

Abstract

The present study was aimed to determine the effect of ATP dose rate on longevity, motility, and viability of frozen-thawed buffalo bull’s sperm. For this purpose, 20 frozen semen straws of four different buffalo bulls were obtained from a private semen production unit (Al-Hiawan Sires, Sahiwal). The experiment was replicated four times. In each replicate, four frozen straws of the same bulls were thawed and pooled. After evaluating the motility, the pooled frozen-thawed sample was aliquoted into three separate tubes containing 0 (control), 1-, or 2-mM concentrations of ATP. Afterward, each aliquot was incubated at 37oC for 4 hrs. The sperm motility, viability, and membrane integrity were observed at various time points (10 min., 1hr, 2hr, and 4hr) during the incubation period of four hrs. It was observed that progressive motility and most of the motion kinematic parameters were greater (P<0.05) in control. A significant (P<0.05) interaction was observed between ATP dose and incubation time. The progressive motility, total motility, VCL, VAP, ALH, and BCF decreased (P<0.05) both by incorporation of ATP (1mM or 2mM) and increasing incubation time (from 10 min to 4hrs) which indicate the ATP and incubation time of 4 hours negatively affect the motion kinetics of buffalo bull sperm. In conclusion, it was witnessed that the motility and plasma membrane integrity of sperm declined by the supplementation of ATP in frozen-thawed buffalo bull semen.

References

Atkinson, D.E., and Walton, G.M. (1967). Adenosine triphos phate conservation in metabolic regulation rat liver citrate cleavage enzyme. J. Biol. Chem., 242(13): 3239-3241.

Bezerra, A.S., Nascimento, T.E.C., Castilho, E.F., Gonçalves, N.L.C., Silva, S.M.B.S., Cardoso, A.S., Souza, H.K.R. and Rodrigues, L.F.S. (2019). Effect of adenosine concen tration on quality of cooled ram semen. R. Bras. Zootec. 48:e20190111.

Foresta, C., Rossato, M., Mioni, R., and Zorzi, M. (1992). Progesterone induces capacitation in human spermatozoa. Andrologia, 24(1): 33-35.

Kinukawa, M., Oda, S., Shirakura, Y., Okabe, M., Ohmuro, J., Baba, S.A., Nagata, M., and Aoki, F. (2006). Roles of cAMP in regulating microtubule sliding and flagellar bending in demembranated hamster spermatozoa. FEBS Letters, 580(5): 1515-1520.

Luria, A., Rubinstein, S., Lax, Y., and Breitbart, H. (2002). Extracellular adenosine triphosphate stimulates acrosomal exocytosis in bovine spermatozoa via P2 purinoceptor. Biol. Reprod., 66(2): 429-437.

Peña, F., Martínez, H.R., Tapia, J., Ferrusola, O.C., Fernandez, G.L., and Garcia, M.B. (2009). Mitochondria in mamma lian sperm physiology and pathology: a review. Reprod. Dom. Anim., 44(2): 345-349.

Tash, J., and Mann, T. (1973). Adenosine 5-Cyclic Monophosphate in Relation to Motility and Senescence of Spermatozoa. Proceedings of the Royal Society of London B: Biological Sciences, 184(1074), 109-114.

Tash, J.S,. and Means, A.R. (1988). cAMP-dependent regulatory processes in the acquisition and control of sperm flagellar movement. Progress in Clinical and Biological Research 267:335-355

Thuwanut, P., Arya, N., Comizzoli, P., and Chatdarong, K. (2015). Effect of extracellular adenosine 5-triphosphate on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality, and in vitro fertilizing ability. Theriogenology 84: 702–709.

Yamashiro, H., Toyomizu, M., Kikusato, M., Toyama, N., Sugimura, S., Hoshino, Y., Abe, H., Moisyadi, S., and Sato, E. (2010). Lactate and adenosine triphosphate in the extender enhance the cryosurvival of rat epididymal sperm. J. Am. Assoc.Lab. Anim. Sci., 49(2), 160-166.

Downloads

Published

2023-12-29

Issue

Vol. 44 No. 2 (2023): The Indian Journal of Animal Reproduction (Dec) 2023

Section

Research Article

How to Cite

Hanif, M., Ahmad, E., Naseer, Z., Akhtar, M.S., Ahmad , T., Hussain, T., & Murtaza , S. (2023). Effect of Adenosine 5´ Triphosphate (ATP) and Incubation Time on Frozen Thawed Buffalo Bull Spermatozoa. The Indian Journal of Animal Reproduction, 44(2), 64–68. https://doi.org/10.48165/ijar.2023.44.02.12
Crossref
Scopus
Google Scholar
Europe PMC