EFFECT OF STORAGE CONDITIONS OF SEMINAL STAINS ON DIFFERENT TEXTURES OF CLOTHS IN RELATION TO DNA YIELD
DOI:
https://doi.org/10.48165/Keywords:
ethidium bromide, biological materialsAbstract
The seminal stains recovered on fabrics are one of the most important evidence in rape cases. Stained clothing’s, undergarments and swab’s on fabric material taken from vagina and surrounding regions, are submitted for DNA Fingerprinting test to compare it with the blood sample of the accused person. Many a times, isolation of DNA from such stains particularly when the stains are old is not possible. The causes could be degradation or decomposition of the biological material, which depends mainly upon the environmental conditions. The other possible factors could be nature of clothing’s. In the present study, it was observed that maximum DNA recovery was from cotton clothing’s even when stored for long durations followed by tricot material.
Downloads
References
1. Jeffreys AJ, Wilson V, Thein SL. Individual-specific 'fingerprints' of human DNA. Nature 316;1985:76-79 2. Raina A, Dogra TD (2003) Methods of collection, Preservation and Forwarding of Biological Material for DNA Fingerprinting. Ind Internet J Forensic Med Toxicol 1(2);2003; www.icfmt.org
3. www.legalserviceindia.com/articles/rape_laws.htm
4. Kido A, Masaaki H, Yasuhisa Y, Hiroshi K, Rie S, Kazuyuki S, Aya T, Masakazu O. Nine short tandem repeat loci analysis in aged semen stains using the Amp FLSTR Profiler Kit and description of a new vWA variant allele. Legal Medicine 5; 2003:93-96
5. Ng DP, Koh D, Choo SG, Ng V and Fu. Effect of storage conditions on the extraction of PCR-quality genomic DNA from saliva. Clin Chim Acta 343; 2004 :191-194
6. Bomjen G, Raina A, Sulaiman IM, Hasnain SE, Dogra TD. Effect of storage of blood samples on DNA yield, quality and fingerprinting: a forensic approach. Indian J Exp Biology 34(4);1996 : 384-386
7. Del Rio
