EFFECT OF DIFFERENT PRESERVATIVES ON THE HUMAN SOFT TISSUES STORED AT DIFFERENT TEMPERATURES AND INTERVALS OF TIME FOR THE ISOLATION OF DNA FOR DNA FINGERPRINTING
DOI:
https://doi.org/10.48165/Keywords:
DNA fingerprinting, PreservativesAbstract
The major problem faced by the forensic laboratories is to get the samples in proper conditions. Most of the biological samples brought for DNA analysis are either partially degraded or completely degraded and many a times it is not possible to extract DNA from them. It necessitates the optimization of the temperature conditions and preservatives for the storage of the biological samples (soft tissues). Our study concluded that -80°C temperature and normal saline as preservative is the best combination for the storage of the forensic samples till processed further. Since –80°C facility is not available in all the labs, the samples stored in normal saline at lower temperature (4°C) is found to be the feasible condition for long storage. Formalin fixation of the samples for long storage is not desirable for the isolation of DNA because first it has to be deformalized and also it hampers the PCR activity.
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References
1. Raina A., Dogra T.D., (Aug 2002) Application of DNA Fingerprinting in Medico legal practice. JIMA, Vol.100 No.8, Page 1-8
2. Lalji Singh (May 1991), DNA profiling and its applications, Current science Vol. 60, pp: 580-585
3. Raina Anupuma & Dogra TD (2003) Methods of collection, preservation and forwarding of biological material for DNA fingerprinting. Indian Internet Journal of Forensic Medicne & Toxicology, vol. 1 No2.
4. Ludes B., Pfitzinger H. And Mangin P., (May 1993) DNA fingerprinting from tissues after variable postmortem periods. Journal of Forensic Science, JFSCA, Vol 38, No 3, pp: 686-690
5. Yang DY., Watt K., (2005) Contamination control when preparing archaeological remains of ancient DNA analysis.” Journal of Archaeological Science, Vol 32, pp: 331-336
6. Lindahl T., (1993) Instability and decay of the primary structure of DNA . Nature, Vol. 362 ,pp: 709-715
7. O'Rourke DH, Hayes MG and Carlyle SW.,(2000) Ancient DNA studies in physical anthropology. Annual Review of Anthropology, Vol. 29, pp: 217-242.
8. Yang, D.Y. (2003) Contamination controls and detection in ancient DNA studies. Acta Anthropologica Sinica, Vol. 22, pp: 163-173.
9. Raina A, Pramanik P & Dogra TD (2005) Effect of storage conditions of seminal stains on different textures of cloths in relation to DNA yield. Indian Internet journal of Forensic Medicine & Toxicology, Vol.3 No.2
Downloaded From IP - 59.178.18.222 on dated 13-Sep-2024
10.Dessauer HC, Reeder TW, Cole CJ, Knight. (1996) A Rapid screening of DNA diversity using Dot-blot
www.IndianJournals.com
technology and Allele-specific oligonucleotides: Maternity of hybrids and unisexual clones of hybrid origin Members Copy, Not for Commercial Sale
(Lizards, Cnemidophorus) Molecular Phylogenetics and Evolution, Vol. 6, pp: 366-372
11.Shrinivasan M, Sedmak D, Jewell S., (2002) Effect of fixative and tissue processing on the contents and integrity of nucleic acids. American Journalof Pathology. Vol.161 No.6.
12.Sambrook and Fritsch (1989) Molecular Cloning: A laboratory manual.” Cold Spring Harbor Laboratory Press, IInd Edition
13.Ginya Bomjen, A Raina, IM Sulaiman, SE Hasnain & TD Dogra, (April 1996) Effect of storage of blood
samples on DNA yeild, quality and fingerprinting: A Forensic Approach. Indian Journal of Experimental
