Genetic Polymorphism at 15 STR Loci in Delhi's (India) population
DOI:
https://doi.org/10.48165/Keywords:
Short tandem repeat (STR), Indian population data, AmpFISTR® Identifiler™ kit, Genetic diversityAbstract
The migration and genetic drift influence the population structures, which necessitate the genetic polymorphism studies of the population subgroups. Delhi, being capital of India, has mixed population group of the individuals migrated from different parts of the country. Keeping this in view, the allelic database of Delhi population is generated here using 15 STR markers to study the impact of these natural factors (migration, genetic drift, selection etc.) on this population. For this purpose, the allelic frequencies for 15 STR loci were estimated and observed to be on higher side. Further, the study includes the calculation of observed and expected heterozygosity. Moreover, the genetic variation statistics shows that there is out breeding in this population and also the effective number of alleles is higher. The observed results indicate that all the 15 STR loci are highly polymorphic in this population which indicates that there is high degree of diversity. Also, it signifies that this is a random mating population. Moreover, it is observed that four loci (D3S1358, TH01, D13S317, and D16S539) are undergoing some sort of selection.
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Ashma R. and Kashyap V. K. 2002a. Genetic study of 15 important STR loci among four major ethnic groups of Bihar, India. J. Forensic Sci. 47, 1139–1142.
Ashma R. and Kashyap V. K. 2002b. Genetic polymorphism at 15 STR loci among three important sub-populations of Bihar, India. Forensic Sci. Int. 130, 58–62.
Bamshad M., Kivisild T., Watkins W. S., Dixon M. E., Ricker C. E., Rao B. B. et al. 2001. Genetic evidence on the origins of Indian caste populations. Genome Res. 11, 994-1004.
Butler J. M. 2001. Forensic DNA Typing. 2nd ed. Academic Press, 12-13.
Das K., Malhotra K. C., Mukherjee B. N., Walter H., Majumder P. P. and Papiha S. S. 1996. Population structure and genetic differentiation among 16 tribal populations of Central India. Hum. Bio. 68(5), 679-705.
Gaikwad S. and Kashyap V. K. 2002. Polymorphism at fifteen hyper-variable microsatellite loci in four populations of Maharashtra, India. Forensic Sci. Int. 126(3), 267–271.
Gill P., Jeffreys A. J. and Werrett D. J. 1985. Forensic applications of DNA fingerprints. Nature 318, 577–579.
Gill P., Kimpton C. P., Urquhart A., Oldroyd N., Millican E. S., Watson S. K. et al. 1995. Automated short tandem repeat (STR) analysis in forensic casework—a strategy for the future. Electrophoresis 16, 1543-1552.
Hammond H. A., Jin L., Zhong Y., Caskey C. T. and Chakarborty R. 1994. Evaluation of 13 Short Tandem Repeat loci for use in personal identification applications. Am. J. Hum. Genet. 55, 175-189.
Hartl D. L. and Clark A. G. 1997. Principles of Population Genetics. 81-82.
Victorian Parliament Law Reform Committee. 2002. Inquiry into Forensic Sampling and DNA Databases: Background / Issue Paper.
Kashyap V. K., Ashma R., Gaikwad S., Sarkar B. N. and Trivedi R. 2004. Deciphering diversity in populations of various linguistic and ethnic affiliations of different geographical regions of India: Analysis based on 15 microsatellite markers. J. Genet. 83(1), 49-63.
Kashyap V. K., Guha S. and Trivedi R. 2002. Concordance study on 15 STR loci in three major populations of Himalayan State Sikkim. J. Forensic Sci. 47(5), 1163–1167.
Manly B. F. J. 1985. The Statistics of Natural Selection on Animal Populations. Chapman and Hall, London. 272-282.
Nei M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York. 159–164.
Nei M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York. 176–187.
Nei M. and Roychoudhury A. K. 1974. Sampling variance of heterozygosity and genetic distance. Genet. 76, 379-390.
Rajkumar R. and Kashyap V. K. 2002. Distribution of alleles of fifteen STR loci of the Powerplex 16™ multiplex in four predominant population groups of South India. Forensic Sci. Int. 126, 175–179.
Reddy B. M., Naidu V. M., Madhavi V. K., Thangaraj K., Langstieh B. T., Venkataramana P. et al. 2005. STR data for the AmpFlSTR Profiler Plus loci among 27 populations of different social hierarchy from southern part of Andhra Pradesh, India. Forensic Sci. Int. 149, 81-97.
Sambrook J., Fritsch E. F. and Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Sarkar N. and Kashyap V. K. 2002. Genetic diversity at two pentanucleotide and thirteen tetranucleotide STR loci by multiplex PCR in four predominant population groups of Central India. Forensic Sci. Int. 128(3), 196–201.
Wright S. 1984. Evolution and the Genetics of Population (Variability within and among Natural Populations). Vol. 4. University of Chicago Press, Chicago.
