Spectrophotometric Study of Yield of DNA from Fresh Blood and Dried Blood Samples
DOI:
https://doi.org/10.48165/Keywords:
DNA isolation, Effect of temperatureAbstract
DNA profiling has attained a pivotal role in forensic identification. It has assumed great significance in terms of exclusion as well as fixation of identity and is being relied upon by law enforcers and judiciary equally. A study was conducted to look at the effect of temperature on blood samples for isolation of DNA at MNL Medical College, Allahabad, U.P. With the increase in temperature yield of DNA decreases in both fresh and dried blood samples. Fresh samples stored at -20°C,0°C,4°C,40°C had 2.38% ,3.47%, 10.19%, 13.79% and dried blood samples had 0.78%, 0.96%, 2.56%, 5.90% decrease in DNA yield after one week.
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References
1. Butler, John M. Forensic DNA Typing. Elsevier, 2001: 14-15.
2. Levy H, Simpson OJ. What the blood really showed. In: Levy H. And the blood dried out. Basic Books, Newyork 1996: 157- 188.
3. www.ornl.gov/hgmis/elsi/forensics.shtml. modified 16,June 2009.
4. Budowle B. Molecular Diagnostic Application in Forensic Science. Elsevier 1995:100-126.
5. www. Calpoy.edu/~/bio/ubl/protocols. modified September 2001.
6. Halsall A, Ravetto P, Reyes Y, Thelwell N, Davidson A, Gaut R, Little S. The quality of DNA extracted from liquid or dried blood is not adversely affected by storage at 4ºC for up to 24 h. International J Epidemiol 2008; 37: 7–10.
7. Mitchell C. HIV-1 DNA yield from dried blood spots is diminished after storage in a humid incubator at 37°C compared to -20°C. J Clin Microbiol 2008; 23: 234- 237.
8. Mc Nally L, Shaler RC, Baird M , Balazs I, De Forest P, Kabilinsky L. Evaluation of DNA isolated from human bloodstains exposed to Ultraviolet Light, Heat, Humidity and Soil Contamination. J Forensic Sci 1989; 34(5): 1059-1069.
9. Bojmen G , Raina A , Sulaiman I M ,Hasanain SE, Dogra T.D. Effect of storage of blood samples on DNA yield, quality and fingerprinting: a forensic approach. Ind J Exp Biol 1996; 34: 384-86.
