Anti-cancer Activities of Methanol Extract of Rhizophora mucronata against Breast Cancer MCF7 Cell Line
DOI:
https://doi.org/10.48165/Keywords:
Rhizophora mucronata,, Cancer, MCF7 cell line, FTIR, MTT, IC50Abstract
Cancer is the uncontrolled growth and spread of abnormal cells, associated with regulation of cell cycle and apoptosis. Plant-derived compound is now considered as the most effective method of cancer treatment. The focus of the current research has been based on the identification of natural and synthetic compound that can be used in the prevention or the treatment of cancer. Methanolic extract of Rhizophora mucronata showed the presence of carbohydrates, phenol, alkaloid, amino acids, fat, flavonoids, glycosides, phenols, protein, saponins, sterols and tannins. Fourier-transform infrared spectroscopy (FTIR) confirmed the presence of primary, secondary amines, amides, alkanes, alkynes, aldehydes, saturated aliphatic, primary amines, alkyl halides, aliphatic amines, carboxylic acids, alkyl halides and alkyne. In 3-(4,5- Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay, at the concentration of 100 µg/mL of the extract, the viability of the cells was 90%. When increasing the concentration of the extract, the viability of the cells was decreased. At the concentration of 1,000 µg/mL, the viability of the cells was 50%. Tryphan blue dye exclusion assay was carried to perform the test for cell viability of the half maximal inhibitory concentration (IC50) (1,000 µg/mL) value was found in the extract. The percentage of viability of Michigan Cancer Foundation-7 (MCF-7) cell line for control is 100%. Based on the result of present study, it can also be concluded that the effect on methanolic extract of R. mucronata has the anticancer activity potential in MCF-7 cell line.
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[1] Agha GA, De Cindio F. Concurrent object-oriented programming and petri nets: advances in petri nets. Springer Science & Business Media; New York. 2001 Apr 18.
[2] Ballabh B, Chaurasia OP. Traditional medicinal plants of cold desert Ladakh used in treatment of cold, cough and fever. Journal of ethnopharmacology. 2007 Jun 13;112(2):341-9.
[3] Bandaranayake WM, Des Rocher A. Role of secondary metabolites and pigments in the epidermal tissues, ripe ovaries, viscera, gut contents and diet of the sea cucumber Holothuria atra. Marine Biology. 1999 Jan 1;133(1):163-9.
[4] Bandaranayake WM. Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wetlands Ecology and Management. 2002 Dec 1;10(6):421-52.
[5] Bandaranayake WM. Traditional and medicinal uses of mangroves. Mangroves and salt marshes. 1998 Sep 1;2(3):133-48.
[6] Bieche I, Lerebours F, Tozlu S, Espie M, Marty M, Lidereau R. Molecular profiling of inflammatory breast cancer. Clinical Cancer Research 2004;10(20):6789–95.
[7] Croce CM. Oncogenes and cancer. New England Journal of Medicine. 2008 Jan 31;358(5):502-11.
[8] Dalilan S, Rezaei-Tavirani M, Nabiuni M, Heidari-Keshel S, Azodi MZ, Zali H. Aqueous extract of lavender angustifolia inhibits lymphocytes proliferation of hodgkin’s lymphoma patients. Iranian journal of cancer prevention. 2013;6(4):201.
[9] Dasgupta T, Rao AR, Yadava PK. Chemomodulatory efficacy of basil leaf (Ocimum basilicum) on drug metabolizing and antioxidant enzymes, and on carcinogen-induced skin and forestomach papillomagenesis. Phytomedicine. 2004 Jan 1;11(2-3):139- 51.
[10] Engel G, Palloff R, Pratt K. Using mobile technology to empower student learning. Inthe Proceedings of 27th Annual Conference on Distance Teaching & Learning 2011.
[11] Gangar SC, Sandhir R, Rai DV, Koul A. Modulatory effects of Azadirachta indica on benzo (a) pyrene
induced forestomach tumorigenesis in mice. World Journal of Gastroenterology: WJG. 2006 May 7;12(17):2749.
[12] Kamboj VP. Herbal medicine. Current science. 2000 Jan 10;78(1):35-9.
[13] Kathiresan K, Ramanathan T. Medicinal plants of Parangipettai coast. Annamalai University, Chennai. 1997.
[14] Kathiresan K. A review of studies on Pichavaram mangrove, southeast India. Hydrobiologia. 2000 Jul 1;430(1-3):185-205.
[15] Knudson AG. Two genetic hits (more or less) to cancer. Nature Reviews Cancer. 2001 Nov 1;1(2):157-62.
[16] Matsumoto A, Jinno H, Ando T, Fujii T, Nakamura T, Saito J, Takahashi M, Hayashida T, Kitagawa Y. Biological markers of invasive breast cancer. Japanese journal of clinical oncology. 2015 Oct 20;46(2):99-105.
[17] Mbaveng AT, Kuete V, Mapunya BM, Beng VP, Nkengfack AE, Meyer JJ, Lall N. Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities. Environmental toxicology and pharmacology. 2011 Sep 30;32(2):162-7.
[18] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods. 1983 Dec 16;65(1-2):55-63.
[19] Nelson HD, Zakher B, Cantor A, Fu R, Griffin J, O’meara ES, Buist DS, Kerlikowske K, van Ravesteyn NT, Trentham-Dietz A, Mandelblatt JS. Risk Factors for Breast Cancer for Women Aged 40 to 49 YearsA Systematic Review and Meta-analysis. Annals of internal medicine. 2012 May 1;156(9):635-48.
[20] Shanmugapriya K, Saravana PS, Payal H, Mohammed S, Williams B. Antioxidant potential of pepper (Piper nigrum Linn.) leaves and its antimicrobial potential against some pathogenic microbes. Indian journal of natural products and resources. 2012;3(4):570-577.
[21] Simon A, Allais DP, Duroux JL, Basly JP, Durand Fontanier S, Delage C. Inhibitory effect of curcuminoids on MCF-7 cell proliferation and structure–activity relationships. Cancer letters. 1998 Jul 3;129(1):111-6.
[22] Tavasoli M, Ghaedi K, Tavasoli Z, Sadeghi M. Signal transduction for cancer treatment. Genetics in the 3rd Millennium 2007;4(4):914–20.
[23] Thirunavukkarasu P, Ramanathan T, Ramkumar L, Shanmugapriya R, Renugadevi G. The antioxidant and free radical scavenging effect of Avicennia officinalis. Journal of Medicinal Plants Research 2011;5(19):4754–8.
