To Individualisation of Subject (Donor) by Determination of Serological Markers from Obtain Biological Fluid at Crime Scene
DOI:
https://doi.org/10.48165/Keywords:
ABH blood group, body fluids, crime scene, serological markers, urineAbstract
For this study samples was collected from 20 donors with unknown blood group and secretor status had been determined from saliva by using biological fluid. ABO typing on the concentrated samples was successfully performed after 1 month of storage. Urine stained clothing samples are often submitted to forensic science laboratories for ABH blood group antigen determination. The serogenetic markers of semen stains submitted can be used to determine the origin of any of these samples. ABH blood group substances have previously been identified from urine. ABH blood group substance is low in urine in comparison with other body fluids.
Downloads
References
[1] An JH, Shin KJ, Yang WI, Lee HY. Body fluid identification in forensics. The Korean Society for Biochemistry and Molecular Biology 2012.Available from: http://dx.doi.org/10.5483/BMBRep.2012.45.10.206
[2] OUP Corrected Proof–Final, 4/12/2011, SPi. Available online:http://global.oup.com/booksites/content/ 9780199594009/9780199594009.
[3] Lattes L. On the practical application of the test for agglutination tor the specific and individual diagnosis of human blood. Institute of Forensic Medicine of the University of Torino, Director: Prof. M. Carrara. Availablr from: https://www.ncjrs.gov/pdffiles1/pr/92422_part4. pdf.
[4] OUP Corrected Proof–Final, 4/12/2011, SPi. Available from:http://global.oup.com/booksites/content/ 9780199594009/9780199594009.
[5] F. Samuel Baechtel. Secreted blood group substances: distributions in semen and stabilities in dried semen stains. Journal of Forensic Sciences 1985;30(4):1119– 29.
[6] Chase MG. ABO typing studies on liquid urine. Journal of Forensic Science 1986;31:881–885.
[7] Roy R. Concentration of urine samples by three different procedures: ABO typing from concentrated urine samples. Journal of Forensic Science 1990;35(5): 1133–7.
[8] Sato I, Nakamura A, Ujiie K, Yukawa N, Nakajima Y. A sand witch enzyme-linked immunosorbent assay for ABO blood typing of semen by using anti-p 84 monoclonal antibody as a marker of blood group substance in semen. Journal of Forensic Science 2000;45(4):95-800.
[9] Bunai Y, Nakamura I, Nagai A, Yamada S, Watanabe Y, Takayama T, et al. Blood grouping of mixed bloodstains using immunocytochemical methods. Journal of Forensic Science 1999;44(1):100–4.
[10] Hamada K, Koreeda A, Ohtsu Y, Yonemitsu K, Tsunenari S. A simple dot enzyme-linked immunosorbent assay for A BO blood typing of biological fluid and stains: effects of heating samples. Legal Medicine (Tokyo) 2002;4(4):217–22.
[11] Morita K, Kimura H. Simple determination methods for blood grouping from biological stains. 2. Immunochromatographic determination of ABO blood groups from saliva specimens. Nihon Hoigaku Zasshi 1998;52(3):191–5.
[12] Shiono H. Personal identification using DNA polymorphism—the identification of forensicbiological materials. Nihon Hoigaku Zasshi 1996;50(5):320–30.
[13] Zachová M, Zelený M, Pexa T, Mazura I, Hirt M. Determination of AB0 blood group system from degraded blood stains on serological and molecular genetic level. Soud Lek 2004;49(3):43–6.
[14] Zlobina NA. Specific research of antigens of the ABO system in samples of decayed blood. Sudebno Meditsinskaia Ekspertiza 2004;47(6):35.
[15] Diadichkina NV, BuragoIuI. The identification of antigens of systems AB0, GM, and haptoglobin (HP) phenotypes in mixed bloodstains of human and domestic goose. Sudebno-Meditsinskaia Ekspertiza 2003;46(4):31–2.
[16] Sakharov RS, Skosyrev GV, Fedulova MV. A rapid polycationic method for typing erythrocyte antibodies in liquid blood and stains. Sudebno-Meditsinskaia Ekspertiza 1997;40(4):29–31.
[17] Thomsen H, Adamzik I. Immunocytochemical determination of ABH and MN antigens on dried blood traces in the nanoliter range. Forensic Science International 1990;48(1):59–69.
[18] Kimura A, Uda T, Nakashima S, Ikeda H, Yasuda S, Osawa M, et al. ABO blood grouping of bloodstains by sandwich ELISA using monoclonal antibody specific for human red cell band 3. International Journal of Legal Medicine 1993;105(4):209–12.
[19] Bunai Y, Nakamura I, Nagai A, Yamada S, Watanabe Y, Takayama T, et al. Blood grouping of mixed bloodstains using immunocytochemical methods. Journal of Forensic Sciences 1999;44(1):100–4.
[20] Zhou B, Guo JY, Wang CX, Chen J. The rapid determination of the ABO group from body fluids (or stains) by dot enzyme-linked immunosorbent assay (dot-ELISA) using enzyme-labelled monoclonal antibodies. Journal of Forensic Sciences 1990; 35(5):1125–32.
[21] De Soyza K. Evaluation of an enzyme linked immunosorbent assay (ELISA) method for ABO and Lewis typing of body fluids in forensic samples. Forensic Science International 1991;52(1):65–76.
[22] Pflug W, Bässler G, Eberspächer B. ABO and Lewis typing of secretion stains on nitrocellulose membranes using a new dot-blot-ELISA technique. Forensic Science International 1989;43(2):171–82.
[23] De Soyza K, Garland DG. Studies and observations on Lewis grouping of body fluids and stains. Forensic Science International 1988;38(1–2):129–37.
[24] Bässler G. Determination of the Lewis blood group substances in stains of forensically relevant body fluids. Forensic Science International 1986;30(1):29– 35.
[25] Piner SC, Sänger MS. Lewis grouping of human secretion stains. Forensic Science International 1980;15(2):87–92.
[26] Pereira M. ABO and Lewis typing of semen, saliva and other body fluids. Haematologia (Budap) 1984;17(2): 317–22.
[27] Lee HY, Park MJ, Kim NY, Yang WI, Shin KJ. Rapid direct PCR for ABO blood typing. Journal of Forensic Science 2011;56(Suppl 1):S179–82. DOI:10.1111/j.1556- 4029.2010.01591.x.
[28] Harrington JJ, Martin R, Kobilinsky L. Detection of hemagglutinins in dried saliva stains and their potential use in blood typing. Journal of Forensic Science 1988;33(3):628–37.
[29] Rao DV, Kashyap VK. A simple dipstick immunoassay for detection of A and B antigens. Journal of Immunoassay 1992;13(1):15–30.
[30] Yazawa S, Ohkawara H, Nakajima T, Hosomi O, Takeya A, Furukawa K. A novel method for blood group typing with the aid of monoclonal antibody immobilized dyed microspheres. Nihon Hoigaku Zasshi 1992;46(6):440– 4.
[31] Saga K. Preparation of partially fixed erythrocytes and its application for immunohematologic examination. Nihon Hoigaku Zasshi 1990;44(2):147–62.
[32] Takizawa N, Ohba Y, Mukoyama R, Komuro T, Mukoyama H, Takei T. Determination of ABO blood groups from saliva and saliva stains by an indirect enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies. Nihon Hoigaku Zasshi 1989;43(4):294–302.
[33] Komuro T, Mukoyama R, Mukoyama H. Application of enzyme-linked immunosorbent assay (ELISA) to the medico-legal Identification. Nihon Rinsho 1995;53(9): 2322–9.
[34] Oepen I. Identification of characteristics in blood and semen stains—a review. Forensic Science International 1988;36(3–4):183–91.
[35] Bolton S, Thorpe J. An examination of a contaminated seminal stain using absorption-elution and enzyme linked immunosorbent assay (ELISA). Journal of Forensic Science 1988;33(3):797–800.
