Postmortem Evaluation of Autolytic Changes in Morphology of Red Blood Cells and Haemogram Pattern for Estimation of Time since Death
DOI:
https://doi.org/10.48165/Keywords:
Autolytic changes, Haemogram, Peripheral blood smear, Red blood cell morphology, Time since death, Post-mortem intervalAbstract
The accurate estimation of time since death sometimes is very important for forensic experts in administration of justice. Various cells and tissues of the body showed significant changes with respect to post-mortem interval and one such sample is blood. The aim of this study is to assess the post-mortem autolytic changes in morphology of red blood cells and haemogram pattern for estimation of time since death. The present study was a cross sectional one and non random purposive sampling was done. The blood samples from 60 dead bodies which were brought for postmortem examination in the mortuary and preserved in <4oC temperature was taken after taking into consideration inclusion and exclusion criteria. The time since death was recorded and studied with respect to autolytic changes in RBC morphology and haemogram pattern. On meticulous examination of all the blood samples there were certain changes observed in the RBCs shape, intactness, central pallor and peripheral redness with respect to post-mortem interval. However, no statistical significance was correlated in the haemogram (RBC count, Hemoglobin, haematocrit, MCV, MCH and MCHC) with respect to time since death.
Downloads
References
[1] Maeda H, Ishikawa T, Michiue T. Forensic biochemistry for functional investigation of death: concept and practical application. Legal Medicine. 2011;13(2):55– 67.
[2] Kumar B, Mahto T, Kumari V. Determination of time since death from changes in morphology of Red Blood Cells. Journal of Indian Academic Forensic Med. 2015;37(2):148–51.
[3] Jat SS, Punia RK, Khichi MK, Sharma S. Effect of time since death on morphological changes of red and white
estimation of postmortem interval. Medico-Legal Update. 2007;7(2): 56–62.
blood cells-an autopsy based study at S.M.S. medical college & attached group of hospitals, Jaipur during the year 2016-2017. Medico-legal Update. 2019;19(2): 145–50.
[4] Penttila A, Laiho K. Autolytic changes in blood cells of human cadavers- Morphological studies. Forensic Science International. 1981;17(2):121–32.
[5] Lahio K, Penttila A. Autolytic changes in blood cells and other tissue cells of human cadavers I. Viability and ion studies. Forensic Science International. 1981; 17:109–20.
[6] Babapulle CJ, Jayasundera NPK. Cellular changes and time since death. Medical Science Law 1993;33(2):213– 22.
[7] Bardale R, Dixit PG. Evaluation of morphological changes in blood cells of human cadaver for the
[8] Shah K, Agarwal SS, Kumar L, Chavali KH. Determining postmortem survival period and blood group antigenicity of red blood cells: a cross- sectional study. Anil Aggrawal’s Internet Journal of Forensic Medicine and Toxicology. 2015;16(2):8.
[9] Kundu SD and Dutta SS. Changes in haemogram in subjects after death as a tool to estimate time passed since death. IOSR Journal of Dental and Medical Sciences. 2017;16(10):19–27.