Detection of "11-Nor-9-carboxy-Δ9 -Tetrahydrocannabinol" in Pubic Hair of Cannabis Abuser: Target Ion GC-MS Method
DOI:
https://doi.org/10.48165/Keywords:
Pubic hair, 11-Nor-9-carboxy-Δ9 -Tetrahydrocannabinol, Drug abuser, Target ion GC-MS, Forensic toxicologyAbstract
Cannabis substance abuse is widely causing addiction across the world. In the drug of abuse cases, hair plays an important role in identifying drug abuse and drug history consumption. However, scalp hair may not be available in every drug abuse cases; other body hairs play an essential role in forensic toxicological analysis. This study attempt has been made to use pubic hair as an alternative specimen for the detection of 11-Nor-9-carboxy-Δ9 - Tetrahydrocannabinol metabolite of cannabis abusers using the target ion by GC-MS method. Pubic hairs of 10 years cannabis abusers were collected and used for GC-MS analysis to detect THC-COOH metabolite. The study confirmed the presence of THC-COOH metabolite with the help of 371 target ions. This method will be useful in identifying the presence of cannabis in drug-facilitated crimes for forensic toxicological analysis. In further, this method would also be useful in identifying single drug exposure cases.
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References
[1] Grotenhermen F. The toxicology of cannabis and cannabis prohibition. Chem Bio Divers. 2007;4:1744– 1769.
[2] UNODC World Drug Report 2020: Global Drug Use Rising; While COVID-19 Has Far Reaching Impact on Global Drug Markets.
[3] Ministry of Social Justice And Empowerment Govt. of India Report 2019: Magnitude of Substance Use in India.http://socialjustice.nic.in/writereaddata/Upload File/Survey%20Report.pdf
[4] Manolis N, Athanasios K, Matthaios P, Elena V, Polychronis D, Katerina K, Alexander I, Emmanouil G, Aristidis M. Comparative Evaluation of Drug Deposition in Hair Samples Collected from Different Anatomical Body Sites, Journal of Analytical Toxicology. 2017;41:214–223.
[5] Offidani C, Strano R, Chiarotti M. Drug Distribution in the Head, Axillary and Pubic Hair of Chronic Addicts. Forensic Science International 1992;63:108-108.
[6] Henderson GL. Mechanism of Drug Incorporation into Hair. Forensic Science International. 1993;63:19-29.
[7] Papoutsis I, Athanasellis S, Nikolaou P, Spiliopoulou C, Tsiola A, Stefanidou M. Post-mortem Hair Analysis Verifies Heroin Exposure and Leads to Killer. Austin Journal of Forensic Science & Criminology. 2015;2(2): 1021.
[8] Mechoulam R. Plant cannabinoids: a neglected pharmacological treasure trove. British Journal of Pharmacology. 2005;146:913–915.
[9] Sharma P, Murthy P, Bharat S. Chemistry, Metabolism and Toxicology of Cannabis: Clinical Implications. Iran Journal of Psychiatry. 2012;7(4):149-156.
[10] Musshoff F, Madea B. Review of biologic matrices (urine, blood, hair) as indicators of recent or ongoing cannabis use. The Drug Monit. 2006;28:155–163.
[11] Boldis O, Kocsis Gy, Ganchali A, Furesz J. Gas Chromatography-Mass Spectroscopy-Single Ion Monitoring Measurement of 11-THC-COOH in Urine. Journal of Chromatographic Science 2003;41(2):45-65.
[12] Altunkaya D, Clatworthy AJ, Smith RN, Start IJ. Urinary cannabinoid analysis: comparison of four immunoassays with gas chromatography-mass spectrometry. Forensic Science International. 1991;50: 15–22.
[13] Fraser AD, Worth D. Monitoring urinary excretion of cannabinoids by fluorescence-polarization immuno assay: a cannabinoid-to-creatinine ratio study. Ther Drug Monit. 2002;24:746–750.
[14] Andrenyak D Moody D, Slawson M, Leary D, Haney M. Determination of Δ-9-Tetrahydrocannabinol (THC), 11-hydroxy-THC, 11-nor-9-carboxy-THCand Cannabi diol in Human Plasma using Gas Chromatography– Tandem Mass Spectrometry. Journal of Analytical Toxicology. 2017;41:277–288.
