Variation in DNA Barcode of Rose Varieties

Authors

  • Rajinder Kaur Assistant Professor, Department of Microbial Biotechnology, Panjab University, Chandigarh-160014, India
  • Navpreet Kaur Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India
  • Geetanjali Vij Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India
  • Lakshay Kalra Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India
  • Lalhruaitluangi Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India
  • Kavita Dhiman Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India
  • Poonam Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India
  • Priyanka Sareen Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India
  • Sanjeev Agrawal Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India
  • Jagdish Rai Research Scholar, Institute of Forensic Science and Criminology, Panjab University, Chandigarh-160014, India

DOI:

https://doi.org/10.48165/

Keywords:

DNA barcoding, rbcl gene, Rubisco, Rose cultivars, Rosa species, Rose phylogenetics, Identification of roses

Abstract

The DNA barcode of a species is expected to vary only 2-7%, among different varieties of the species. There are more than 4000 morphologically different varieties of roses that evolved due to the selection pressure of the aesthetic choices of humans. The extensive use of roses in decoration, bouquet, as well as in medicine and food also increase the value of their species identification from forensic trace samples. DNA barcoding-based identification of species is emerging as a robust forensic technique. Here we have determined the DNA barcode of a few prominent varieties of rose and estimated variation among them as well as their phylogenetic relationships. The rbcl gene does not vary significantly among these rose varieties, at most, it can identify the class/group of a rose variety. 

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References

[1] Akey JM, Ruhe AL, Akey DT, et al. Tracking footprints of artificial selection in the dog genome. National Academy of Sciences. 2010;107:1160–1165.

[2] Raymond O, Gouzy J, Just J, et al. The Rosa genome provides new insights into the domestication of modern roses. Nature Genetics. 2018;50:772–777.

[3] Crawler.Gbif.Org. The Plant List with literature [Internet]. GBIF Secretariat; 2016 [cited 2020 May 1]. Available from: http://www.gbif.org/dataset/dedf296e-7bc6-48cf-9225- 9cd600a0d2ef.

[4] Smulders MJM, Arens P, Bourke PM, et al. In the name of the rose: a roadmap for rose research in the genome era. Hortic Res [Internet]. 2019 [cited 2020 May 1];6. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC6499834/[5] World Federation of Rose Societies, 2014 Directory [Internet]. [cited 2020 May 1]. Available from: https:// archive.org/stream/WorldFederationOfRoseSocieties2014/ World%20Federation%20of%20Rose%20Societies% 2C%202014_djvu.txt.

[6] Pal BP. The rose in India. New Delhi: Indian Council of Agricultural Research; 2011.

[7] Ratnasingham S, Hebert PDN. BARCODING: bold: The Barcode of Life Data System (http://www.barcodinglife.org): BARCODING. Molecular Ecology Notes. 2007;7:355–364.

[8] Jan CH, Byrne DH, Manhart J, et al. Rose Germplasm Analysis with RAPD Markers. Horticulture Science. 1999;34:341–345.

[9] Scariot V, Akkak A, Botta R. Characterization and Genetic Relationships of Wild Species and Old Garden Roses Based on Microsatellite Analysis. Journal of the American Society for Horticultural Science. 2006;131:66–73.

[10] Koopman WJM, Wissemann V, De Cock K, et al. AFLP markers as a tool to reconstruct complex relationships: A case study in Rosa (Rosaceae). American Journal of Botany, 2008;95:353–366.

[11] Fougère-Danezan M, Joly S, Bruneau A, et al. Phylogeny and biogeography of wild roses with specific attention to polyploids. Annals of Botany. 2015;115:275–291.

[12] Wissemann V, Ritz CM. The genus Rosa (Rosoideae, Rosaceae) revisited: molecular analysis of nrITS-1 and atpB rbcL intergenic spacer (IGS) versus conventional taxonomy. Botanical Journal of the Linnean Society. 2005;147:275– 290.

[13] Wu S, Ueda Y, He H, et al. Phylogenetic Analysis of Japanese Rosa Species Using matK Sequences. Breed Science. 2000;50:275–281.

[14] Pang X, Song J, Zhu Y, et al. Applying plant DNA barcodes for Rosaceae species identification. Cladistics. 2011;27:165– 170.

[15] Grand Mogul Rose [Internet]. [cited 2020 Apr 29]. Available from: https://www.helpmefind.com/rose/l.php?l=2.21595.0.

[16] Full text of The Indian Rose Annual XXXI, 2015 [Internet]. [cited 2020 Apr 29]. Available from: https://archive.org/ stream/TheIndianRoseAnnualXXXI2015/The++Indian+ Rose+Annual+XXXI%2C+2015_djvu.txt.

[17] Young MA, American Rose Society. Modern roses 12: the comprehensive list of roses in cultivation or of historical or

botanical importance. Shreveport, LA: American Rose Society; 2007.

[18] Phillips R, Rix M. The quest for the rose. 1st U.S. ed. New York: Random House; 1993.

[19] rose | Description & Major Species | Britannica [Internet]. [cited 2020 Apr 29]. Available from: https://www.britannica. com/plant/rose-plant#ref247259.

[20] Society AR, editor. Handbook for Selecting Roses A Rose Buying Guide from the American Rose Society. American Rose Society; 1980.

[21] Moody M. Mary Moody’s roses: more than 200 of the world’s most beautiful and hardy roses. Sydney: Murdoch Books; 2006.

[22] Phillips R, Rix M. The ultimate guide to roses: a comprehensive selection. New York: Macmillan; 2004.

[23] Chomczynski P, Mackey K, Drews R, et al. DNAzol ®/ : A Reagent for the Rapid Isolation of Genomic DNA. BioTechniques. 1997;22:550–553.

[24] Dereeper A, Guignon V, Blanc G, et al. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Research. 2008;36:W465-469.

[25] Rice P, Longden I, Bleasby A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet TIG. 2000;16:276–277.

[26] Gaurav A, Banyal N, Raju DVS, et al. Genetic characterization of Rosa species using morphological markers. Indian Journal of Agriculture Science. 2018;88:1396–1402.

[27] Rai H, Raju D, Kumar Mb A, et al. Characterization and analysis of genetic diversity among different species of rose (Rosa species) using morphological and molecular markers. Indian Journal of Agriculture Science. 2015;85:240–245.

[28] Wallinger C, Juen A, Staudacher K, et al. Rapid Plant Identification Using Species- and Group-Specific Primers Targeting Chloroplast DNA. PLOS ONE. 2012;7:e29473.

[29] Liu C, Wang G, Wang H, et al. Phylogenetic Relationships in the Genus Rosa Revisited Based on rpl16, trnL-F, and atpB rbcL Sequences. Horticulture Science. 2015;50:1618–1624.

Published

2021-04-30

How to Cite

Variation in DNA Barcode of Rose Varieties. (2021). Indian Internet Journal of Forensic Medicine and Toxicology, 19(1&2), 11–15. https://doi.org/10.48165/