A Review of Green Technologies for Cellulose Extraction from Lignocellulosic  Biomass: Pretreatment Methods and Applications

Authors

  • Muhammad Asif Department of Energy and Environment, Sindh Agriculture University, Tandojam, Pakistan
  • Arshad Ali Department of Mechanical Engineering, BUITEMS, Quetta, Pakistan.
  • Abdul Ghaffar Bugti Mir Chakar Khan Rind University, Sibi, Balochistan, Pakistan.
  • Namatullah Department of Chemical Engineering, BUITEMS, Quetta, Pakistan.
  • Mohammad Siddique Department of Chemical Engineering, BUITEMS, Quetta, Pakistan.
  • Saadatullah Khan Suri Department of Chemical Engineering, BUITEMS, Quetta, Pakistan
  • Muladad Department of Mechanical Engineering, BUITEMS, Quetta, Pakistan
  • Anwar khan Department of Civil Engineering, Polytechnic College Quetta, Balochistan, Pakistan

DOI:

https://doi.org/10.48165/gjs.2026.3107

Keywords:

Green energy, Energy crises, Biomass sources, Biomass

Abstract

The majority of the material called lignocellulose biomass (LCB), a type of plant  biomass, is made up of these three compounds. The most common polymer to break  down on Earth is cellulose, which is very useful and has many uses. Because cellulose  is securely linked to the compound’s hemicellulose and lignin in lignocellulosic  materials, it is highly challenging to separate the material from LCB components;  therefore, effective pretreatment techniques are required to make the material  separation easier. Green pretreatment approaches have been proposed as a gentler and  more environmentally responsible way to address these problems. When assessing  these green processes, a variety of factors are taken into account, such as the  effectiveness of delignification with a lower solvent demand, the impact on the  environment, the economic feasibility, and the suitability for new sustainable  processing solutions. We also assessed how using biomass affected land use,  production of greenhouse gases, and group effects, among other socioeconomic and  environmental factors. This paper examines the challenges, ongoing research, and  recent developments in effective biomass-based energy systems to increase energy  output while lowering environmental concerns. Additionally, it provides a summary of  how cellulose is separated from various lignocellulosic biomass using sustainable and  green methods. 

 

References

[1]. Meng, X., Wang, Y., Conte, A. J., et al. (2023). Applications of biomass-derived solvents in biomass pretreatment—Strategies, challenges, and prospects. Bioresource Technology, 368, 128280. https://doi.org/10.1016/j.biortech.2022.128280

[2]. Abolore, R. S., Jaiswal, S., & Jaiswal, A. K. (2023). Green and sustainable pretreatment methods for cellulose extraction from lignocellulosic biomass and its applications: A review. Carbohydrate Polymer Technologies and Applications, 22, 100396. https://doi.org/10.1016/j.carpta.2023.100396

[3]. Prasad, B. R., Padhi, R. K., & Ghosh, G. (2023). A review on key pretreatment approaches for lignocellulosic biomass to produce biofuel and value-added products. International Journal of Environmental Science and Technology, 20(6), 6929–6944. https://doi.org/10.1007/s13762-022-04252-2

[4]. Roy, S., & Chundawat, S. P. (2023). Ionic liquid–based pretreatment of lignocellulosic biomass for bioconversion: A critical review. BioEnergy Research, 16(1), 263–278. https://doi.org/10.1007/s12155-022-10425-1[5]. Güleç, F., Parthiban, A., Umenweke, G. C., Musa, U., Williams, O., Mortezaei, Y., Suk-Oh, H., Lester, E., Ogbaga, C. C., Gunes, B., & Okolie, J. A. (2023). Progress in lignocellulosic biomass valorization for biofuels and value-added chemical production in the EU. Biofuels, Bioproducts and Biorefining. https://doi.org/10.1002/bbb.2544

[6]. Mujtaba, M., Fraceto, L., Fazeli, M., et al. (2023). Lignocellulosic biomass from agricultural waste to the circular economy. Journal of Cleaner Production, 402, 136815. https://doi.org/10.1016/j.jclepro.2023.136815

[7]. Gallego-García, M., Moreno, A. D., Duque, A., et al. (2023). Recent advances on physical technologies for the pretreatment of food waste and lignocellulosic residues. Bioresource Technology, 369, 128397. https://doi.org/10.1016/j.biortech.2022.128397

[8]. Areeya, S., Panakkal, E. J., Yasurin, P., et al. (2023). Chemical pretreatment of lignocellulosic biomass for the production of bioproducts. Applied Science and Engineering Progress, 16(3). https://doi.org/10.14416/j.asep.2023.02.008[9]. Kumar, N., Sharma, R., Saharan, V., & Aggarwal, N. K. (2023). Enhanced xylanolytic enzyme production from Parthenium hysterophorus. 3 Biotech, 13(12), 396. https://doi.org/10.1007/s13205-023-03817-3

[10]. Reis, C. E., Milessi, T. S., Ramos, M. D., et al. (2023). Lignocellulosic biomass-based glycoconjugates for diverse biotechnological applications. Biotechnology Advances, 68, 108209. https://doi.org/10.1016/j.biotechadv.2023.108209

[11]. Kumar, A., Sood, A., Maiti, P., et al. (2023). Lignin-containing nanocelluloses as renewable alternatives. Current Opinion in Green and Sustainable Chemistry, 100830. https://doi.org/10.1016/j.cogsc.2023.100830

[12]. Ji, Q., Yu, X., Chen, L., et al. (2023). Comprehensive depolymerization of lignin from lignocellulosic biomass. Critical Reviews in Environmental Science and Technology, 1–22. https://doi.org/10.1080/10643389.2023.2190314[13]. Rodrigues Reis, C. E., Milessi, T. S., Ramos, M. D., et al. (2023). Lignocellulosic biomass-based glycoconjugates for diverse applications. Biotechnology Advances. https://doi.org/10.1016/j.biotechadv.2023.108209

[14]. Amesho, K. T., Lin, Y. C., Mohan, S. V., et al. (2023). Deep eutectic solvents in the transformation of biomass into biofuels. Environmental Chemistry Letters. https://doi.org/10.1007/s10311-022-01521-x

[15]. Sharma, S., Bangotra, R., & Bajaj, B. K. (2023). Application of nanomaterials for biofuel production from lignocellulosic biomass. In Nanotechnology for Advanced Biofuels (pp. 189–212). Elsevier. https://doi.org/10.1016/B978-0-323-91759-9.00005-8

[16]. Pang, Y. L., Lim, S., Lai, S. O., et al. (2023). Green chemistry for biomass conversion into cellulose and bioethanol. In Green Sustainable Process for Chemical and Environmental Engineering and Science (pp. 121–137). Elsevier. https://doi.org/10.1016/B978-0-323-95183-8.00003-2[17]. Harrison, T. R., Gupta, V. K., Alam, P., et al. (2023). From trash to treasure: Sustainable cellulosic materials from waste streams. International Journal of Biological Macromolecules, 233, 123511. https://doi.org/10.1016/j.ijbiomac.2023.123511

[18]. Riseh, R. S., Vazvani, M. G., Hassanisaadi, M., et al. (2024). Agricultural waste as a source for cellulose preparation. Industrial Crops and Products, 208, 117904. https://doi.org/10.1016/j.indcrop.2023.117904

[19]. Namasivayam, S. K., Prakash, P., Babu, V., et al. (2023). Aquatic biomass cellulose fabrication into nanocomposite for water purification. Journal of Cleaner Production, 136386. https://doi.org/10.1016/j.jclepro.2023.136386

[20]. Srivastava, R. K., Nedungadi, S. V., Akhtar, N., et al. (2023). Effective hydrolysis for waste plant biomass. Science of the Total Environment, 859, 160260. https://doi.org/10.1016/j.scitotenv.2022.160260[21]. Przypis, M., Wawoczny, A., & Gillner, D. (2023). Biomass and cellulose dissolution in renewable materials treatment. Applied Sciences, 13(2), 1055. https://doi.org/10.3390/app13021055

[22]. Pham, C. D., Dang, M. D., Ly, T. B., et al. (2023). Extraction methods and applications of lignin-silica hybrids. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2023.123175

[23]. Dongare, P. P., & Pawar, H. S. (2023). Biohydrogen production from lignocellulosic biomass fermentation. In Green Approach to Alternative Fuel for a Sustainable Future (pp. 223–242). Elsevier. https://doi.org/10.1016/B978-0-12-824318-3.00019-9

[24]. Ab Rasid, N. S., Shamjuddin, A., Rahman, A. Z., et al. (2021). Recent advances in green pretreatment methods for lignocellulosic biomass. Journal of Cleaner Production, 321, 129038. https://doi.org/10.1016/j.jclepro.2021.129038[25]. Mankar, A. R., Pandey, A., Modak, A., et al. (2021). Pretreatment of lignocellulosic biomass: Recent advances. Bioresource Technology, 334, 125235. https://doi.org/10.1016/j.biortech.2021.125235

[26]. Nasar, M. S., Soomro, S. A., Aziz, S., et al. (2022). Green pretreatment method and lignin extraction from biomass. Journal of Applied and Emerging Sciences, 12(2), 90–97.

[27]. Soomro, S. A., & Ahmad, H. (2021). Lignocellulosic biomass and bioenergy production in Pakistan. Journal of Chemistry and Nutritional Biochemistry, 2(2), 46–58.

[28]. Sankaran, R., Cruz, R. A., Pakalapati, H., et al. (2020). Pretreatment of microalgal and lignocellulosic biomass. Bioresource Technology, 298, 122476.[29]. Vieira, S., Barros, M. V., Sydney, A. C., et al. (2020). Sustainability of sugarcane biomass pretreatment for bioethanol production. Bioresource Technology, 299, 122635.

[30]. Prasad, B. R., Padhi, R. K., & Ghosh, G. (2023). Pretreatment approaches lignocellulosic biomass. International Journal of Environmental Science and Technology.

[31]. Lobato-Peralta, D. R., Duque-Brito, E., Villafan-Vidales, H. I., et al. (2021). Trends in lignin extraction and valorization. Journal of Cleaner Production, 293, 126123.

[32]. Siddique, M., Ahmed, S., Aziz, S., et al. (2021). Advances in lignocellulosic derived biofuels. Jurnal Kejuruteraan, 33(2), 165–173.[33]. Pawlak, J. J., Ford, E., & Gonzalez, R. (2024). Textiles from non-wood feedstocks: Challenges and opportunities of current and emerging fiber spinning technologies. Journal of Bioresources and Bioproducts, 9(4), 410–432.

[34]. Rajendran, K., Drielak, E., Sudarshan Varma, V., et al. (2018). Pretreatment of lignocellulosic feedstocks for bioenergy production. Biomass Conversion and Biorefinery, 8, 471–483.

[35]. Rahmati, S., Doherty, W., Dubal, D., et al. (2020). Pretreatment and fermentation of lignocellulosic biomass. Reaction Chemistry & Engineering, 5(11), 2017–2047.

[36]. Akram, H. A., Imran, M., Javaid, A., et al. (2023). Catalytic conversion of lignocellulosic biomass using metal organic frameworks. Molecular Catalysis, 539, 112893.[37]. Zhang, N., Han, Q., & de Vries, B. (2021). Circularity assessment framework in construction industry. Sustainability, 13(22), 12466.

[38]. Guiao, K. S., Tzoganakis, C., & Mekonnen, T. H. (2022). Green mechano-chemical processing of lignocellulosic biomass. Chemosphere, 293, 133647.

[39]. Mujtaba, M., Fraceto, L. F., Fazeli, M., et al. (2023). Agricultural waste lignocellulosic biomass in circular economy. Journal of Cleaner Production, 402, 136815.

[40]. Zhang, Y., Ding, Z., Hossain, M. S., et al. (2023). Advances in lignocellulosic biomass pretreatment and biorefinery approaches. Bioresource Technology, 367, 128281.[41]. Dhara, S., Samanta, N. S., Uppaluri, R., et al. (2023). High-purity alkaline lignin extraction from Saccharum ravannae. International Journal of Biological Macromolecules, 234, 123594.

[42]. Esquivel-Hernández, D. A., García-Pérez, J. S., López-Pacheco, I. Y., et al. (2022). Resource recovery of lignocellulosic biomass waste into lactic acid. Journal of Environmental Management, 301, 113925.

[43]. Pham, C. D., Dang, M. D., Ly, T. B., et al. (2023). Lignin-silica hybrid extraction methods and applications. International Journal of Biological Macromolecules, 230, 123175.

[44]. Yoo, C. G., Meng, X., Pu, Y., & Ragauskas, A. J. (2020). Role of lignin in lignocellulosic biomass conversion. Bioresource Technology, 301, 122784.[45]. Chen, W. H., Naveen, C., Ghodke, P. K., Sharma, A. K., & Bobde, P. (2023). Co-pyrolysis of lignocellulosic biomass with carbonaceous materials. Fuel, 345, 128177.

[46]. Thi, N. B., Lin, C. Y., & Kumar, G. (2016). Valorization of food waste to hydrogen and methane. Journal of Cleaner Production, 122, 29–41.

[47]. Nahak, B. K., Preetam, S., Sharma, D., et al. (2022). Net-zero energy production from lignocellulosic biomass. Renewable and Sustainable Energy Reviews, 161, 112393.

[48]. Wang, F., Ouyang, D., Zhou, Z., Page, S. J., Liu, D., & Zhao, X. (2021). Lignocellulosic biomass for power generation and energy storage. Journal of Energy Chemistry, 57, 247–280.[49]. Raud, M., Kikas, T., Sippula, O., & Shurpali, N. J. (2019). Challenges in lignocellulosic biofuel production technology. Renewable and Sustainable Energy Reviews, 111, 44–56.

[50]. Fatma, S., Hameed, A., Noman, M., et al. (2018). Lignocellulosic biomass as sustainable bioenergy source. Protein and Peptide Letters, 25(2), 148–163.

[51]. Ullah, K., Sharma, V. K., Dhingra, S., et al. (2015). Lignocellulosic biomass resources in developing countries. Renewable and Sustainable Energy Reviews, 51, 682–698.

[52]. Hoang, A. T., Nižetić, S., Ong, H. C., Mofijur, M., et al. (2021). Microwave pretreatment technologies for biomass conversion. Chemosphere, 281, 130878.[53]. Sharma, H. K., Xu, C., & Qin, W. (2019). Biological pretreatment of lignocellulosic biomass for biofuels. Waste and Biomass Valorization, 10, 235–251.

[54]. Rahardjo, A. H., Azmi, et al. (2021). Pretreatment of tropical lignocellulosic biomass for industrial biofuel production. IOP Conference Series: Materials Science and Engineering, 1053, 012097.

[55]. Prasad, A., Sotenko, M., et al. (2016). Life cycle assessment of lignocellulosic biomass pretreatment methods. International Journal of Life Cycle Assessment, 44–50.

[56]. Ab Rasid, N. S., Shamjuddin, A., Rahman, A. Z., et al. (2021). Green pretreatment methods for lignocellulosic biomass. Journal of Cleaner Production, 321, 129038.[57]. Mahmood, H., et al. (2019). Advances in pretreatment of lignocellulosic biomass for biofuels. Current Opinion in Green and Sustainable Chemistry, 20, 18–24.

[58]. Bharadwaj, A. S., Dev, S., Zhuang, J., et al. (2023). Chemical pretreatment of lignocellulosic biomass using low-liquid catalysts. Bioresource Technology, 368, 128339.

[59]. Malik, K., Sharma, P., Yang, Y., et al. (2022). Advanced pretreatment and fermentation approaches for lignocellulosic bioethanol. Industrial Crops and Products, 188, 115569.

[60]. Wang, W., & Lee, D. J. (2021). Lignocellulosic biomass pretreatment by deep eutectic solvents. Bioresource Technology, 339, 125587.

Published

2026-05-15

How to Cite

A Review of Green Technologies for Cellulose Extraction from Lignocellulosic  Biomass: Pretreatment Methods and Applications. (2026). Global Journal of Sciences, 3(1), 67-81. https://doi.org/10.48165/gjs.2026.3107