In-vitro regeneration in long-day garlic (Allium sativum)

Rajni Karn; J K Ranjan; Pragya Ranjan; B Das; B L Attri

doi:10.48165/

Authors

Rajni Karn ICAR-DOGR, Pune
J K Ranjan ICAR-IARI, New Delhi
Pragya Ranjan ICAR NBPGR, New Delhi
B Das ICAR Res. Complex for NEH Region RS, Tripura
B L Attri ICAR-DMR, Solan

DOI:

https://doi.org/10.48165/

Keywords:

In-vitro regeneration, (Allium sativum), multiplication of garlic, transformed

Abstract

The cloves of garlic (Allium sativum L.) cultivar Mukteshwar Local, after disinfection, were sprouted under in-vitro condition (85.00 % sprouting) on MS medium containing 2 mg/l GA3. The cloves were soaked in 100 ppm GA3 solution for 24 hr. Maximum number of shoot buds (7.0) was recorded in treatment containing MS + 4.0 ppm BAP + 1.0 ppm NAA. Among different treatments for shoot multiplication, treatment MS+5.0 ppm BAP+ 1.0 ppm KIN + 0.5 ppm GA3 was the best. The number of shoots also varied with the level of sugar, light intensity and, light and dark cycle on shoot multiplication. They multiplied best in 30 g/l sucrose, 5000 lux light intensity and 16:8 hr of light and dark cycle. The developed protocol may be used for mass multiplication of garlic cultivars as well as regeneration of genetically transformed cell/ tissue.

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References

Bhojwani S S and Razdan M K. 1996. Plant Tissue Culture: Theory and Practice. Elsevier, Amsterdam, 502 pp.

Camara M C, Vandenbergue L P S, Rodrigues C, Oliveira J, Faulds C, Bertrand E and Soccol C R. 2018. Current advances in gibberellic acid (GA3) production, patented, technologies and potential applications. Planta 248: 1049-

, https://doi.org/10.1007/s00425-018-2959-x George E F. 1993. Plant propagation by tissue culture. Part I : The Technology. Exegetcn Ltd., Edington, England. Hammerschag F A. 1978. Influence of light intensity and date of explantation on growth of geraniam callus. HortScience, 13 : 153-54.

Haque M S, Tomikichi W and Kazumi H. 2003. Shoot

Regeneration and Bulblet Formation from Shoot and Root Meristem of Garlic Cv Bangladesh Local. Asian Journal of Plant Sciences 2 (1): 23-27.

Haramaki C. 1971. Tissue culture of Gloxinia Comb. Proc. Intl. Plant Prop. Soc. 21: 442-48.

Lazzeri P A and Dunwell J M. 1986. In vitro regeneration from seedling organ of Brassica oleracea var. Italica Plenk cv. Green Coment II. Effect of light conditions and explant size. Ann. Bot, 58 : 699-710.

Mcgranachau, G H , Driver J A and Tulcke W. 1987. Tissue culture of Juglans. In: Cell and Tissue Culture in Forestry. Vol. 3 Bonga, D.M. and Durzan, D. J. (Eds).

Passi R, Dhatt A S and Sidhu M K. 2018. In vitro micropropagation in tropical short day onion (Allium cepa l.). Bangladesh J. Bot. 47(4): 961-967.

Rizza A, Walia A, Lanquar V et. al. 2017. In vivo gibberellin gradients visualized in rapidly elongating tissues. Nature Plants 3, 803–813. https://doi.org/10.1038/s41477-017- 0021-9

Salisbury F B and Ross C W. 1995. Hormones and Growth Regulators: Auxin and Gibberellins. Plant Physiology. CBS Pub. Delhi. 309-329.

Suarez Padrón, I. E., Pérez Meza, P. M., & López Díaz, C. M. (2020). Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae). Ciencia Tecnología Agropecuaria 21(2), e1193. https://doi.

org/10.21930/rcta.vol21_num2_art:1193

Taize L and Zeiger E. 2002. Gibnberellins. In : Plant Physiology. 2 edn.: 591-620.