Effect of scarification on biochemical activities of aged   seeds in guava (Psidium guajava )

Santosh Kumari; Arvind Malik; Rajesh Mor; Sonu Kumar; M L Jat; Jaipal Jaipal

doi:10.48165/chr.2023.11.2.14

Authors

Santosh Kumari Department of Horticulture, College of Horticulture, Maharana Pratap Horticultural University, Karnal -132 001, Haryana, India
Arvind Malik Department of Horticulture, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar- 125 004, Haryana, India
Rajesh Mor Department of Horticulture, College of Horticulture, Maharana Pratap Horticultural University, Karnal -132 001, Haryana, India
Sonu Kumar Department of Horticulture, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar- 125 004, Haryana, India
M L Jat Department of Horticulture, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar- 125 004, Haryana, India
Jaipal Jaipal Department of Horticulture, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar- 125 004, Haryana, India

DOI:

https://doi.org/10.48165/chr.2023.11.2.14

Keywords:

Seed quality, Scarification, Peroxidase, Dehydrogenase, Electrical conductivity

Abstract

The study was carried out on fresh, one, two and three year aged seeds of guava (Psidium guajava L. ) L-49 (Sardar) in the laboratoryof Department of Seed Science and Technology, CCS HAU, Hisar, during 2018-2019. The seeds were subjected to different seed scarification treatments, viz. hot water treatment at 80°C for 1 minute, concentrated sulphuric acid for quick dip and water soaking for 48 hr. The seeds were assessed for peroxidase activity, dehydrogenase activity and electrical conductivity. Hot water treatment at 80°C for 1 minute resulted into maximum peroxidase activity (710.0) and dehydrogenase activity (0.233) but minimum electrical conductivity (11.02). It is concluded that seeds treated with hot water treatment at 80°C for 1 minute showed maximum peroxidase, dehydrogenase activities and minimum electrical conductivity which reflects that better seed quality.

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References

AOSA 1983. Seed Vigor Testing Handbook. East Lansing.

Ahmadvand G, Soleimani F, Saadatian B and Pouya M. 2012. Effects of seed priming on germination and emergence traits of two soybean cultivars under salinity stress. International Research Journal of Applied Basic Science 3(2): 234-241.

Cavallaro V, Maucieri C and Barbera A C. 2014. Lolium multiflorum Lam. cvs germination under simulated olive mill wastewater salinity and pH stress. Ecological Engineering 71: 113-17.

Bairwa S K, Singh Navab, Verma Harish and Asarey Ram. 2020. Analysis of attitude of fruit growers towards guava (Psidium guajava) cultivation in Sawai Madhopur district of Rajasthan, India. Current Horticulture 8(1): 33-36.

Cakmal T, Atici O , Agar1G and Sunar S. 2010. Natural aging related biochemical changes in alfalfa (Medicago sativa L.) seeds stored for 42 years. International Journal of Plant Sciences 1:1001-006.

Chang S M and Sung J M. 1998. Deteriorative changes in primed sweet corn seeds during storage. Seed Sci. Technol 26:613-26.

Kalyani M., Bharad S G and Parameshwar P. 2014. Effect of growth regulators on seed germination in guava. International Journal on Biological Sciences 5: 81-91.

Kapoor N , Arya A , Siddiqui, Mohd. Asif , Kumar H and Amir A. 2011. Physiological and biochemical changes during seed deterioration in aged seeds of rice (Oryza sativa L). American Journal of Plant Physiology 6(1):28-35.

Kalsa K K., Tomer R P S and Abebie B.2011.Effects of storage duration and hydro-priming on seed germination and vigour of Common vetch. Journal of Science and Development 1(1): 65-73.

Lamichhane J R., Debaeke P , Steinberg C , You M. P, Barbetti M J and Aubertot J N. 2018. Abiotic and biotic factors affecting crop seed germination and seedling emergence: a conceptual framework. Plant and Soil 432: 1-28.

Machado neto N B , Custodio C C and Takaki M. 2001. Evaluation of naturally and artificially aged seeds of Phaseolus vulgaris L. Seed Sci. Technol 29:137-149.

Maldonado-Arciniegas F , Ruales C , Caviedes M., Ramírez D X and León-Reyes A. 2018. An evaluation of physical and mechanical scarification methods on seed germination of Vachellia macracantha (Humb. & Bonpl. ex Willd.) Seigler & Ebinger. Acta Agronómica 67: 120-125. https://doi.

org/10.15446/acag.v67n1.60696

Radha B.N , Channakeshava B C , Hullur N , Bhanuprakash K., Vishwanath K., Umesha, Divya B and Sarika G. 2014. Change in storage enzymes activities in natural and accelerated aged seed of maize (Zea mays L.). International Journal of Plant Sciences 9(2): 306-311.

Shannon L , Kay E , and Lew J. 1966. Peroxidase Isozymes from Horseradish Roots. I. Isolation and Physical Properties. Journal of Biological Chemistry 241(9): 2166–72.

Singh, S, Singh A K and Joshi H K. 2005.Prolong storability of Indian gooseberry (Emblica officinalis Gaertn.) under semiarid ecosystem of Gujarat. Indian Journal of Agricultural Science 75: 647-50.

Tabatabaei S A. 2015.The changes of germination characteristics and enzyme activity of barley seeds under accelerated ageing. Cercetari Agronimice in Moldova. 48(2): 61-67.