Efficient in-vitro regeneration protocol in chrysanthemum  (Chrysanthemum morifolium) from ray florets

D P Jadhav; N R Dalal; T N Saha; G B  Kadam; P R Jadhav; R D Nimbalkar; A A Bhagat

doi:10.48165/

Authors

D P Jadhav ICAR- Directorate of Floricultural Research, Pune, Maharastra, India
N R Dalal ICAR- Directorate of Floricultural Research, Pune, Maharastra, India
T N Saha ICAR- Directorate of Floricultural Research, Pune, Maharastra, India
G B Kadam ICAR- Directorate of Floricultural Research, Pune, Maharastra, India
P R Jadhav ICAR- Directorate of Floricultural Research, Pune, Maharastra, India
R D Nimbalkar ICAR- Directorate of Floricultural Research, Pune, Maharastra, India
A A Bhagat ICAR- Directorate of Floricultural Research, Pune, Maharastra, India

DOI:

https://doi.org/10.48165/

Keywords:

Chrysanthemum, in-vitro, ray florets, callus induction, regeneration, proliferation

Abstract

The experiment was conducted to standardize regeneration protocol from ray florets in chrysanthemum cv. Pusa Arunodaya (TQP 06). For callus induction, the highest callusing percent (82.67%) with a minimum number of days for callus initiation (10.87 days) was observed in MS medium supplemented with Kinetin (10 mg/l) + NAA (1.0 mg/l) in treatment (T3). Highest shooting percent (69.33 %) with minimum number of days for shoot initiation (6.27 days) along with maximum number of shoots per explant (5.67 shoots) in MS medium supplemented with Kinetin (5.0 mg/l) + NAA (1.0 mg/l). In shoot proliferation, the highest number of shoots after 30 days (51.67), 60 days (72.00), and 90 days (98.67) in MS medium supplemented with Kinetin (5.0 mg/l) + NAA (1.0 mg/l). The highest rooting percent (85.00%) with least days for root initiation (7.00 days) along with maximum roots per shoot (8.00) and optimum root length (5.00 cm) in MS medium supplemented with IBA (0.2 mg/l). The rooted plants were successfully acclimatized in 3-4 weeks and survived under field conditions.

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