Characterization and Identification of Atypical Yeast Species Causing Fungemia by MALDI-TOF MS Technique

Authors

  • Raj Singh Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India
  • Sushil Kumar Upadhyay Assistant Professor, Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India
  • Indu Sharma Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India
  • Ashwanti Devi 5Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India
  • Pooja Sharma Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India
  • Neha Chauhan Department of Medical Microbiology, College of Paramedical Sciences, Shri Guru Ram Rai University, Dehradun, Uttarakhand 248001, India
  • Chhaya Singh Department of Botany, Govt. Degree College, Thallisain, Pauri-Garhwal, Uttarakhand 246285, India

DOI:

https://doi.org/10.48165/

Keywords:

MALDI-TOF MS, Fungemia, Candidemia, Rare yeast, Candida species, Rapid diagnosis

Abstract

The incline healthcare services and improvements in  technology have elevated resistance or confrontation  amongst microorganisms. The microbial community has  witnessed evolution by emerging variant yet similar strains  which are hard to detect and treat. An upsurge in drug  resistance has hit at last; overuse of drugs should be  prohibited otherwise the future is about to come when there  will be no antibiotic left to control an illness. Prescription of  suitable drugs must be mandatory following accurate  diagnostics to the disease causative agents or factors. The  Matrix Assisted Laser Desorption Ionization-Time of Flight  Mass Spectrometry (MALDI-TOF MS) represents new era of  diagnostics and medical microbiology. This study focus on  utilization of MALDI-TOF MS technique for characterization  and identification of rare yeast species as clinical isolates  screened from critically ill patients with fungal infection. The  commencement of antifungal resistance in a wide spectrum  of atypical yeasts causing fungemia in several hospitals of  India is worrisome. The rising of hospital patients with  compromised immunity are giving way to pathogenic fungi  leading to death of thousands patients. At lower differential  neutrophills count, the invasion of uncommon yeast Candida  albicans (Fungi: Saccharomycetaceae) be able to root a  systemic infection lead to surplus growth of pathogenic rare  yeasts in many organs, and possibly fatal. At this stage,  physicians’ often recommend anti-fungal drugs to keep the  controlled yeast populace in patients. But over the time, main  challenge to medical experts during treatment is the  development or emergence of resistance in rare yeast species  against the prescribed drugs, insisting medical and  pharmaceutical scientists to design and formulate more  potent drugs and device rapid, truthful diagnostic  techniques. 

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Published

2020-07-12

Issue

Vol. 36 No. 1 (2020): Bio-Science Research Bulletin (Jan-Jun) 2020

Section

Articles

How to Cite

Characterization and Identification of Atypical Yeast Species Causing Fungemia by MALDI-TOF MS Technique . (2020). Bio Science Research Bulletin, 36(1), 55–63. https://doi.org/10.48165/