Protein Profiling and Precursor-product Relationship between Vitellogenin and Lipovitellin in the African catfish, Clarias gariepinus

Authors

  • Pooja Vijay Department of Zoology, University of Delhi, Delhi-110007, India.
  • Luni Sharma MaitreyiCollege, University of Delhi, Delhi 110021, India.
  • Neeta Sehgal Department of Zoology, University of Delhi, Delhi-110007, India

DOI:

https://doi.org/10.48165/

Keywords:

Clarias gariepinus, Vitellogenin, Lipovitellin, Gel filtration chromatography, PAGE, MALDI TOF/MS

Abstract

Nutrients are deposited in the eggs as yolk to  provide energy and to build embryonic  structures. Lipovitellin, the major egg-yolk  protein was purified from the oocytes of Clarias gariepinus. The amino acid sequences of lipovitellin (Lv) peptides obtained by Mass  spectrometry were mapped on its precursor protein, Vitellogenin (Vg). Advanced proteomic tools, PAGE {one dimensional (1-D) andtwo dimensional (2-D)} and mass spectrometry were employed to characterise Vg. Similarity in  sequences of peptidesre-established the  precursor-product relationship between Vg and  Lv. Partial mRNA transcript of vg gene was translated into amino acid sequence that corresponded to conserved domains of  Vitellogenin in NCBI database. Phylogenetic analysis of the nucleotide sequence of Africancat fish vg gene revealed a close similaritywith the  fishes of the order Siluriformes. 

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Published

2019-11-11

Issue

Vol. 38 No. 2 (2019): Bulletin of Pure and Applied Sciences Zoology (Jul-Dec) 2019

Section

Articles

How to Cite

Protein Profiling and Precursor-product Relationship between Vitellogenin and Lipovitellin in the African catfish, Clarias gariepinus . (2019). Bulletin of Pure & Applied Sciences- Zoology , 38(2), 67–81. https://doi.org/10.48165/