Effect of Pottia lanceolata Linnaeus (Moss Extract) on Trichostrongylus in Sheep

Authors

  • Y Sunila Kumari Assistant Professor, Department of Zoology, Osmania University College for Women, (OUCW), Kothi, Hyderabad, Telangana 500095; India
  • Padmavathi Sriram Founder and Proprietor, Nautilus Life sciences, (Zoology Research Laboratory and Consultancy) K.K Nagar, Chennai, Tamil Nadu 600078; India

DOI:

https://doi.org/10.48165/

Keywords:

Moss extract, Pottia lanceolata, Trichostrongyle, Anthelmintic, mRNA, Ttranscriptome analysis, GEO, Nutraceutical, Sheep Nematode

Abstract

Bryophyte species, Pottia lanceolata was used as  anthelmintic, against sheep nematode Trichostrongylus  in vitro. Aqueous extract of Pottia lanceolata exhibited  anthelmintic activity in dose dependent manner  showing maximum efficacy at 20 mg/ml  concentration. To test the efficacy of the treatment  transcriptome analysis was done after isolating and  extracting the mRNA. Gene expression pattern were  studied in both the control and treated samples. A  total of 1,763 genes were expressed in the control and  treated samples, the genes expressed in control and  treated samples differed. It was found that certain  genes were up regulated in the control (257) and  certain genes were down regulated (341). The  transcriptome data as mRNA sequences were  submitted to public database, GEO. The following are  the ids of the submitted sequences. (GSE137609)  Sheep Nematode Control (GSM4083097) Sheep  Nematode treated (GSM4083098). The treatment was  found to be quite effective, as expression of unique  genes was observed, which seem to play a major role  in apoptosis, cuticle damage, neuronal destruction,  and triggering major signaling pathways, causing  obliteration of worms. The bryophyte extract seems to  be effective in inhibiting the nematode activity by  impacting the genome and gradually leading to the  extermination of the parasite and therefore may be  suggested to be used as an effective nutraceutical for  this helminth infection. 

Downloads

Download data is not yet available.

References

Kebede, A.(2019) Review on anthelmintic drug resistance nematodes and its methods of detection in Ethiopia. J Vet Med Animal Sci. 2(1): 1013.

Maurer, V., Hoerdegen, P. and Hertzberg, H. (2007). Reducing anthelmintic use for the control of internal parasites in organic livestock systems. In: Cooper, J., Niggli, U. and Leifert, C. (eds.). Handbook of Organic Food Safety and Quality. Woodhead Publishing Limited, Cambridge, England. Pp. 221-240.

Maingi N, Bjom H, Dangolla A. (1998). The relationship between faecal egg count reduction and the lethal dose 50% in the egg hatch assay and larval development assay. VetParasitol 77:133–145

Paraud C, Kulo A, Pors I, Chartier C. (2009). Resistance of goat nematodes to multiple anthelmintics on a farm in France. Vet Rec; 164: 563–564.5. Dhanalakshmi H, Jagannath MS, D’Souza Placid E. (2003). Multiple anthelmintic resistance in GI nematodes of sheep. J Vet Parasitol. 17(2): 89–91

Jeyathilakan N, Radha G, Gomathinayagam S, John L.(2003). Emergence of anthelmintic resistance in nematodes of sheep in Tamil Nadu. J Vet Parasitol. 17:159–160

Deepa CK, Devada K. (2007). Anthelmintic resistance in GI nematodes of goats. J Vet Anim Sci. 38: 52–54.

Easwaran C, Harikrishnan TJ, Raman M. (2009). Multiple anthelmintic resistance in GI nematodes in South India. Vet Arch. 79: 611–620

Singh Thakur, Kumar, Dinesh and Tandan, Surendra (2008). Paralytic effect of alcoholic extract of Allium sativum and Piper longum on liver amphistome, Gigantocotyle explanatum. Indian Journal of Pharmacology 40(2):64-8

Chaudhary B.L. and Prem Kumar (2011). Anti-microbial activity and preliminary phytochemical screening of epiphytic moss Stereophyllum ligulatum, International Journal of Pharma and Biosciences, 2(40).

Van Hoof, L.D, Vanden Berghe, D.A., Petit E. , Vlietnick A.J. (1981). Antimicrobial and antiviral screening of bryophyte Fitoterapia, 52 (5): 223-229

Gunatilakaa, A.A.L, Kingston D.G.I., Johnson R.K (1994) Mechanism-based isolation and structures of some anticancer active natural products Pure Appl Chem, 66 (10–11): 2219-2222. 13. Sunila Kumari, Y. (2016). Evaluation of in vitro Anthelmintic activity of Bryophyte Pottia lanceolata, International Journal of Innovative Science, Engneering and Technology, 3(6). 14. Hrckova G, Velebny S. (2013). Pharmacological Potential of Selected Natural Compounds in the Control of Parasitic Diseases. Vienna: Springer; Parasitic helminths of humans and animals: health impact and control; pp 29–99.

Roeber F, Kahn L. (2014). The specific diagnosis of gastrointestinal nematode infections in livestock: Larval culture technique, its limitations and alternative DNA-based approaches. Vet Parasitol. 205: 619–628

Ajaiyeoba EO, Onocha PA, Olarenwaju OT. (2001). In vitro anthelmintic properties of Buchholziacoriaceae and Gynandropsisgynandra extract. Pharm Biol. 39: 217- 20. 17. Chandra S, Chandra D, Barh A, Pankaj, Pandey RK, IP (2017). Bryophytes: Hoard of remedies, an ethno-medicinal review, Journal of Traditional and Complementary Medicine, 7(1): 94-98.

Agnieszka L, Yoshinori A (2020). Terpenoids and Aromatic Compounds from Bryophytes and their Central Nervous System Activity; Current Organic Chemistry; Volume 24, Issue 1 19. Faria J., Inês L., Nuno S., Sandra MR, Joana T, and Anabela CDS (2016). Leishmania infantum Asparagine Synthetase A Is Dispensable for Parasites Survival and Infectivity; PLoS Negl Trop Dis.

Faisal Hammad, Mekky Koua, Kazuhide Kimbara, and Akio Tani (2015). Bacterial-biota dynamics of eight bryophyte species from different ecosystems. Saudi J Biol Sci. 22(2): 204–210 21. Nichols DA, Renslo AR, Chen Y (2014) Fragment-based inhibitor discovery against β lactamase. Future Medicinal Chemistry, 6(4): 413-42

Harris AG, Hinds FE, Beckhouse AG, et al. (2002). Resistance to hydrogen peroxide in Helicobacter pylori: role of catalase (KatA) and Fur, and functional analysis of a novel gene product designated 'KatA-associated protein', KapA (HP0874) Microbiology. 148: 3813–25.

Joshi, Phalgun B , Ben L Kelly, Shaden Kamhawi, David L Sacks, W Robert McMaster (2002). Targeted gene deletion in Leishmania major identifies leishmanolysin (GP63) as a virulence factor Mol Biochem Parasitol, 120(1): 33-40.

Jarmie Garcia-Anoveros, Ma C, & Chalfie M (1995). Regulation of Caenorhabditis elegans degenerin proteins by a putative extracellular domain. Curr Biol, 5: 441-8.

Pyati PS, Bell HA, Fitches E, Price DRG, Gatehouse AMR, Gatehouse JA (2009). Cathepsin L like cysteine proteinase (DcCathL) from Delia coarctata (wheat bulb fly): Basis of insecticidal activity; Insect Biochemistry and Molecular Biology; 39; 8

Culetto E, Howard A Baylis, Janet E Richmond, Andrew K Jones, John T Fleming, Michael D Squire, James A Lewis, David B Sattelle (2004). The Caenorhabditis elegans unc-63 gene encodes a levamisole-sensitive nicotinic acetylcholine receptor alpha subunit. J Biol Chem ; 279(41): 42476-83.

Downloads

Published

2020-12-15

Issue

Vol. 39 No. 2 (2020): Bulletin of Pure and Applied Sciences Zoology (Jul-Dec) 2020

Section

Research Paper

How to Cite

Effect of Pottia lanceolata Linnaeus (Moss Extract) on Trichostrongylus in Sheep . (2020). Bulletin of Pure & Applied Sciences- Zoology , 39(2), 503–509. https://doi.org/10.48165/