Glycogen Content and In Vitro Glycogen Consumption in Sheep Cestode Moniezia expansa (Rudolphi, 1805)
DOI:
https://doi.org/10.48165/Keywords:
Moniezia, Glycogen, Cestode, Helminth, SheepAbstract
Moniezia expansa is common cestode in the small intestine of sheep and causes considerable damage to it. Therefore, an effort to study such biological aspects of Moniezia expansa which would help in its control is much needed. The glycogen content and in vitro consumption of endogenous glycogen was determined in immature, mature and gravid proglottids of Moniezia expansa by Anthrone method by Carroll et al. (1956). The total glycogen (on fresh weight basis) was found to be 3.03%, 4.26% and 3.85% respectively in immature, mature and gravid proglottids of unstarved (control) parasite. The glycogen content in immature, mature and gravid proglottids of parasites starved for 3 hrs. is 2.51%, 3.09% and 2.91% respectively, parasites starved for 6 hrs is 1.91%, 2.0% and 2.0% respectively, parasites starved for 9 hrs. 1.25%, 1.33% and 1.35 respectively and parasites starved for 12 hrs is 0.79%, 0.80% and 0.99% respectively (on fresh weight basis).The endogenous glycogen consumption (in percent of total glycogen content of control parasite) by immature, mature and gravid proglottid was found to be 17.16%, 27.46% 24.42% respectively in parasites starved for 3 hours, 36.96%, 53.05% and 48.05% respectively in parasites starved for 6 hours, 58.75% 68.78% and 64.94% respectively in parasites starved for 9 hours and 73.93%, 81.22% and 74.29% respectively in parasites starved for 12 hours. Rate of consumption of endogenous glycogen between different starvation time periods (in percentage per hour) for immature, mature and gravid proglottids is also calculated. Rate of consumption was maximum during early hours of starvation i.e. between 0-3 hrs and 3-6 hrs and was minimum at 9-12 hrs. Glycogen is the major energy reserve in parasitic helminths which live in low oxygen condition, as they are the best substrate for anaerobic energy production. The study of consumption of endogenous glycogen in cestode during starvation period may be quite helpful for their control in the host. The rate of endogenous glycogen consumption is helpful in understanding metabolic rate and physiological aspects of different proglottids of the parasite.
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Carroll, N.V.; Longley, R.W. and Roe, J.H. (1956). The determination of glycogen in liver and muscle by use of Anthrone Reagent. J. Biol. Chem. 220: 583-593.
Chopra, A.K. (1981) Glycogen content and its distribution in three cyclophyllidean cestodes of sheep. Comp. Physiol. Ecol. 6: 173-176.
Chopra, A.K. and Tayal, S. (1993 publ. 1994) Alkaline and acid phosphatase activities in relation to glycogen content in three cestodes of sheep. Indian J. Helminthol. 45(1/2): 100-103. 4. Daugherty, J.W. (1955). The effect of fasting and alloxan treatment of the host on the glycogen gradient in Hymenolepis. J. Parasitol. 41: 30.
Daugherty, J.W. and Taylor, D. (1956) Regional distribution of glycogen in the rat tapeworm, Hymenolepis diminuta. Exp. Parasitol. 5: 376-390.
Fairbairn, D.; Wertheim, G; Harpur, R.P. and Schiller, E.L. (1961). Biochemistry of normal and irradiated strains of Hymenolepis diminuta. Exp. Parasitol. 11: 248-263.
Goodchild, C.G. (1961a) Carbohydrate contents of the tapeworm Hymenolepis diminuta from normal, bile less and starved rats. J. Parasitol. 47: 401-405.
Goodchild, C.G. and Vilar- Alvarez C.M. 1962. Hymenolepis diminutain surgically altered hosts. II. Physical and chemical charges in tapeworms grown in shortened small intestines. J. Parasitol 48: 379-383.
(Rudolphi, 1805): II: Glucidos. Rev. Iber. Parasitol. 25(3/4): 299-319.
Nanware, Sanjay Shamrao, Dhondge, R.M., Kadam, M.S. and Bhure, Dhanraj Balbhim. (2010) Studies on glycogen profile of avian cestode . The Asian Journal of Animal Science. 4(2); 170- 173.
Nanware, Sanjay Shamrao and Bhure, Dhanraj Balbhim. (2011) Studies on glycogen profile of cestodes of Capra hircus . International Multidisciplinary Research Journal. 1(10); 22-24. 12. Premvati, G. and Tayal, S. (1978). Glycogen content and in vitro glycogen consumption in Stilesia globipunctata (Rivolta, 1874). Indian J. Parasitol. 2: 73-75.
Premvati, G. and Tayal, S. (1982).In-vitro estimation of glycogen content in three sheep cestodes. Folia Parasitol.29:259-263
Read, C.P. (1956). Carbohydrate metabolism of Hymenolepis diminuta. Exp. Parasitol. 5: 325-344. 15. Read, C.P. (1967). Carbohydrate metabolism of Hymenolepis (Cestoda). J. Parasitol. 53: 1023- 1029.
Read, C.P. and Rothman, A.H. (1957b). The role of carbohydrates in the biology of cestodes II. The effects of starvation on glycogenesis and glucose consumption in Hymenolepis. Exp. Parasitol. 6: 280-287.
Read, C.P. and Simmons, J.E. Jr. (1963). Biochemistry and Physiology of tapeworms. Physiol. Rev. 43: 263-305.
Reid, W.M. (1942). Certain nutritional requirements of the fowl cestode, Raillietina cesticillus (Molin) as demonstrated by short periods of starvation of the host. J. Parasitol. 28: 319-340. 19. Roe, J.R.; Bailey, J.M.; Gray, R.R. and Robinson, J.N. (1961). Complete removal of glycogen from tissues by extraction with cold TCA. J. Biol. Chem. 236: 1244-1246.
Sai Kumari, M.R.S. and Rao, R. (1991). Glycogen content in the cestode Neokrimia singhia parasitic in Peridcula asiatica. Indian J. Helminthol. 43(2): 129-133.
Smorodinzew, I.A. and Bebeschin, K.W. (1935). The content of glycogen in tapeworms (Cestoids). C.R. Acad. Sci. U.R.S.S. 8:413-414.
Smorodinzew, I.A. and Bebeschin, K.W. (1936a). Bectragezurchemia der Heminthen (Sic). Mitteilung III. Die Chemische Zusammensetzung des Taeniasolium. J. Biochem. (Tokyo) 23: 19- 20.
Smyth J.D. (1962) Studies on tapeworm physiology. X. Axenic cultivation of the hydatid organism Echinococus granulosus; establishment of a basic technique. Parasitology. 52: 441-457.
Smyth, J.D. (1969) The physiology of cestodes. Ist edition. Oliver and Boyd. Edinburgh. 25. Smyth, J.D. and Mc Manus, D.P. (1989) The physiology and biochemistry of cestodes. Cambridge University Press. Cambridge.
Von Brand, T. (1933) Untersuchungeniiber den stoff best and einigercestoden und den stoffwechsel von Moniezia expansa. Z.Vergl. Physiol. 18: 562-596.
Von Brand, T. (1973) Biochemistry of parasites. 2nd Edition. Academic Press. N.Y. 28. Von Brand, T. (1979) Biochemistry and physiology of endo parasites. Elsevier North Holland Biomedical Press: Amsterdam.
Von Brand, T. and Bowman. I.B.R. (1961) Studies on the aerobic and anaerobic metabolism of larval and adult Taenia taeniaeformis. Exp. Parasitol. 11: 276-297.
Von Brand, T.; Churchwell, F. and Eckert, J. (1968). Aerobic and anaerobic metabolism of larval and adult Taenia taeniaeformis: V. Glycogen synthesis, metabolic end products and carbon balances of glucose and glycerol utilization. Exp. Parasitol. 23: 309-318.
Waghmare, S. and Chavan, R.(2010).Some quantitative studies of carbohydrate metabolites in cestode parasite of Gallus gallus domesticus. Int. J. Parasitol. Res., 2, 1-4.
Wardle Wardle, R.A. (1937a) The physiology of the sheep tapeworm, Moniezia expansa Blanchard. Can. J. Res. Sec. D. Zool. Sci. 15(6): 117-126.
Weinland, E. (1901) Ueber den Glykogengehalteiniger parasitischerwurmer. Z. Biol. 41: 69-74. 34. Zainad, S., Shaikh, J.D. and Dama, L.B.(2017) A comparative study of Raillietina echinobothria, Raillietina cesticillus, Raillietina Tetragonl and Choanotaenia infundibulum the intestinal parasites of Gallus domesticus on the glycogen content. Trends in Parasitology research 6(2):5-8.
