Rapid Identification of Clinical Isolates of Klebsiella pneumoniae using MALDI-TOF MS from North India

Authors

  • Sunil Kumar Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India
  • Zainab Saifi Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India
  • Anil Kumar Sharma Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India
  • Sushil Kumar Upadhyay Assistant Professor, Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana 133207, India

DOI:

https://doi.org/10.48165/

Keywords:

Klebsiella pneumoniae, Multi-drug resistance, MALDI-TOF MS, Antibiotic susceptibility testing

Abstract

Infections caused by multi-drug resistant (MDR)  Klebsiella pneumoniae are increasing day by day. K.  pneumoniae poses a severe public health concern  and causes wide array of healthcare associated  infections with limited treatment options. The  quick detection of these isolates is of prime  importance for the adoption of proper antibiotic  treatment and control measures. Also the mis identification using standard lab-based methods  is quite common and hence, the clinical  significance of K. pneumoniae complex members is  inaccurately defined. Here, authors evaluated the  potential of MALDI-TOF (Matrix-Assisted Laser  Desorption Ionization-Time of Flight) MS (Mass  Spectrometry) to discriminate and quick  identification K. pneumoniae complex members.  Thus, this study aimed at full MALDI-based  approach to rapidly detect the K.  pneumoniae isolates up to species level. We also  explored the antibiotic resistance profile of K.  pneumonia isolates using CLSI (Clinical and  Laboratory Standards Institute) guidelines. These  results demonstrate the potential of MALDI-TOF  MS for precise identification of K.  pneumoniae complex members and could be  effectively utilized in the routine diagnostic  microbiology laboratories. 

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Published

2020-06-21

Issue

Vol. 39 No. 1 (2020): Bulletin of Pure and Applied Sciences Zoology (Jan-Jun) 2020

Section

Articles

How to Cite

Rapid Identification of Clinical Isolates of Klebsiella pneumoniae using MALDI-TOF MS from North India . (2020). Bulletin of Pure & Applied Sciences- Zoology , 39(1), 194–199. https://doi.org/10.48165/