Study of Stress, Anxiety and Depression in Type 2 Diabetic Model of Zebrafish (Danio rerio)

Authors

  • Shovit Ranjan Department of Zoology, Miranda House, University of Delhi, Delhi 110007, India.
  • Praveen Kumar Sharma Department of Life Sciences, Central University of Jharkhand, Brambe, Ranchi, Jharkhand 835205, India.

DOI:

https://doi.org/10.48165/

Keywords:

Type 2 Diabetes, Hyperglycemia, Zebrafish, Stress, Anxiety, Depression, Novel tank teST, Light-dark box test

Abstract

Type 2 Diabetes (T2D), which causes  hyperglycemia, affects the central nervous  system, leading to certain neurobehavioral  disorders. These days, zebrafish (Danio rerio) has  emerged as a promising model organism for  experimental studies of neurobehavioral  disorders like stress, anxiety and depression. The  aim of this study was to investigate the effects of  high sucrose induced hyperglycemia or T2D on  stress, anxiety as well as depression like behavior  of zebrafish. Hyperglycemia was induced in  adult zebrafish by immersion in 83.25 mM  sucrose solution for 14 days after performing the  study for survival in sucrose solutions. The  animals were divided into 2 groups in replicates:  control and sucrose-treated hyperglycemic  groups. Afterwards, the behavioral performance  was evaluated in both the groups using novel  tank diving test and light-dark box test.High sucrose induced hyperglycemic group produced  robust anxiogenic effects like increased latency,  reduced exploration in the top of the tank,  increased erratic movements and freezing  behavior in the novel tank diving test and  scototaxis like behavior in the light-dark box test.  Overall, our results confirm that an unfamiliar  environment of high-sucrose induced  hyperglycemia in the zebrafishes can evoke  relatively simple, yet robust stress, anxiety and  depression-like behavioral responses. 

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References

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Published

2020-05-15

Issue

Vol. 39 No. 1 (2020): Bulletin of Pure and Applied Sciences Zoology (Jan-Jun) 2020

Section

Articles

How to Cite

Study of Stress, Anxiety and Depression in Type 2 Diabetic Model of Zebrafish (Danio rerio) . (2020). Bulletin of Pure & Applied Sciences- Zoology , 39(1), 25–35. https://doi.org/10.48165/