Antioxidant and Antihyperglycemic Potential of Luffa acutangula Fruit Extract in Streptozotocin-induced Diabetic Rats
DOI:
https://doi.org/10.48165/Keywords:
Luffaacutangula, Streptozotocin, Antioxidant Enzymes, Antidiabetic EffectAbstract
Our earlier study demonstrates that the treatment of acrude extract of Luffaacutangula (L.) Roxb. (Family: Cucurbitaceae), a folkloric hypoglycaemic plant widely used in the southern part of India which reduces glucose level in blood under high glucose load, and in STZ-provoked diabetes in rats. This study was carried out to establish the antioxidant effects of crude extracts of L. acutangula (50 and 100 mg/kg) in STZ provoked diabetic animals. Regular treatment of the fruit extracts of L. acutangula (50 and 100 mg/kg) for 28 days ensued noteworthy decrease in the levels of plasma TBARS, hydroperoxide, and ceruloplasmin and a substantial increase in plasma GSH, vitamins C and α-tocopherol.Based on the findings, L. acutangulais potential antihyperglycemic agents and restored all the antioxidant factors close to normal range. Thus, our outcomes open new prospects for advanced studies, including, a clinically based study to appraise the antidiabetic potential of the herbs.
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Baker, H., Frank, O., Angelis, B.D., Feingold, S. (1951). Plasma tocopherol in man at various times after ingesting free or acetylated tocopherol. Nutr.Rep. Int. 21: 531-536.
Beutler, E., Duron, O., Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 61: 882-888.
Council, N.R. (2011). Guide for the Care and Use of Laboratory Animals: Eighth Edition. The National Academies Press, Washington, DC.
du Vigneaud, V., Karr, W.G. (1925). Carbohydrate utilization: i. rate of disappearance of d-glucose from the blood. J. Biol. Chem. 66: 281-300.
Ganesan, K., Banu, G.S., Maheswaran, R., Rema, S., Pandian, M.R., Murugesan, A. (2007a). Effect of Plumbago zeylanica L. on blood glucose and plasma antioxidant status in STZ Diabetic Rats. J. Nat. Remed. 7: 66-71.
Ganesan, K., Banu, G.S., Murugesan, A.G.(2008a). Anti-diabetic activity of Helicteres isora L . Bark Extracts on Streptozotocin-induced Diabetic Rats.
Int. J. Pharma. Sci. Nanotechnol. 1: 379-382.
Ganesan, K., Banu, G.S., Murugesan, A.G.(2011). Influence of Helicteres isora L . bark extracts on glycaemic control and renoprotective activity in streptozotocin-induced diabetic rats. Int. J. Pharma. Sci. Nanotechnol. 1: 379-382.
Ganesan, K., Banu, G.S., Murugesan, A., Pandian, M.R. (2007b). Antihyperglycaemic and antiperoxidative effect of Helicteres isora L. bark extracts in streptozotocin
induced diabetic rats. J. Appl. Biomed. 5, 97-104.
Ganesan, K., Banu, G.S., Murugesan, A.G. (2008b). Effect of Helicteres isora bark extracts on heart antioxidant status and lipid peroxidation in streptozotocin diabetic rats. J. Appl. Biomed. 6, 89-95.
Ganesan, K., Banu, G.S., Murugesan, A.G., Pandian, M.R. (2006). Hypoglycaemic effect of Helicteres isora bark extract in rats. J.
Ethnopharmacol. 107, 304-307.
Ganesan, K., Banu, G.S., Murugesan, A.G., Pandian, M.R. (2007c). Antihyperglycaemic and antiperoxidative effect of Helicteres isora L. bark extracts in streptozotocin
induced diabetic rats. J. Appl. Biomed. 5, 97-104.
Ganesan, K., Banu, G.S., Pandian, M.R. (2005). Evaluation of the antioxidant activity of Trianthema portulacastrum L. Indian J. Pharmacol. 37, 331-333.
Ganesan, K., Banu, G.S., Pandian, M.R. (2007d). Effect of Helicteres isora. bark extracts on brain antioxidant status and lipid peroxidation in streptozotocin diabetic rats. Pharma. Biol. 45, 753 -
Ganesan, K., Chung, S.K., Vanamala, J., Xu, B. (2018a). Causal relationship between diet-induced gut microbiota changes and diabetes: A novel strategy to transplant Faecalibacterium prausnitzii in preventing diabetes. Int.
J. Mol. Sci. 19, 3720.
Ganesan, K., Banu,G.S., Murugesan, A.G., Pandian, M.R. (2007e). Effect of Helicteres isora Bark Extract on Protein Metabolism and Marker Enzymes in Streptozotocin-Induced Diabetic Rats. Iranian J. Pharma. Res. 6, 123-129.
Ganesan, K., Gani, S.B. (2014). Relationship between ABO, Rh Blood Groups and Diabetes Mellitus, obesity in Namakkal town, Tamilnadu. Int. J. Adv. Pharma. Biol. Chem. 3,995-998.
Ganesan, K., Jayachandran, M., Xu, B., (2018b). A critical review on hepatoprotective effects of bioactive food components. Crit. Rev. Food Sci. Nutr.
, 1165-1229.
Ganesan, K., Jayachandran, M., Xu, B. (2020a). Diet-Derived phytochemicals targeting colon cancer stem cells and microbiota in colorectal cancer. Int. J.
Mol. Sci. 21, 3976.
Ganesan, K., Ramasamy, M., Gani, S.B. (2013). Antihyperlipideamic effect of Solanum trilobatum L. leaves extract on streptozotocin induced diabetic rats. Asian J. Biomed. Pharma. Sci. 3, 51-57.
Ganesan, K., Ramkumar, K.M., Xu, B. (2020b). Vitexin restores pancreatic β cell function and insulin signaling through Nrf2 and NF-κB signaling
pathways. Eur. J. Pharmacol. 888, 173606.
Ganesan, K., Sukalingam, K., Xu, B. (2017). Solanum trilobatum L. ameliorate thioacetamide-induced oxidative stress and hepatic damage in albino rats. Antioxidants (Basel), 6, 68.
Ganesan, K., Xu, B. (2017a). A critical review on polyphenols and health benefits of Black Soybeans. Nutrients 9, 455.
Ganesan, K., Xu, B. (2017b). Molecular targets of vitexin and isovitexin in cancer therapy: a critical review. Ann. N. Y. Acad. Sci. 1401, 102-113.
Ganesan, K., Xu, B. (2017c). Polyphenol-Rich Dry Common Beans (Phaseolus vulgaris L.) and Their Health Benefits. Int. J. Mol. Sci. 18, 2331.
Ganesan, K., Xu, B., (2017d). Polyphenol-Rich Lentils and Their Health Promoting Effects. Int. J. Mol. Sci. 18, 2390.
Ganesan, K., Xu, B. (2017e). Telomerase Inhibitors from Natural Products and Their Anticancer Potential. Int. J. Mol. Sci. 19,13.
Ganesan, K., Xu, B. (2018). A critical review on phytochemical profile and health promoting effects of mung bean (Vigna radiata). Food Sci. Human Wellness 7, 11-33.
Ganesan, K., Xu, B. (2019). Anti Diabetic Effects and Mechanisms of Dietary Polysaccharides. Molecules 24, 2556.
Gill, N., Arora, R., Kumar, S. (2011). Evaluation of Antioxidant, Anti inflammatory and Analgesic Potential of the Luffa acutangula Roxb. Var. amara. Res. J. Phytochem. 5, 201-208.
Hunashal, J.R.T., D., R., 2012. A Study on antimicrobial activity of extracts of Luffa acutangula var amara fruits. Int. J.Pharma.Bio Sci. 3, 678-685.
Islam, T., Ganesan, K., Xu, B., 2019. New Insight into mycochemical profiles and antioxidant potential of edible and medicinal mushrooms: A review. Int.J.Med.Mushrooms 21, 237-251.
Jadhav, V.B., Thakare, V.N., Suralkar, A.A., Deshpande, A.D., Naik, S.R. (2010). Hepatoprotective activity of Luffa acutangula against CCl4 and rifampicin induced liver toxicity in rats: a
biochemical and histopathological evaluation. Indian J. Exp. Biol. 48, 822- 829.
Jadhav, V.B., Thakare, V.N., Suralkar, A.A., Naik, S.R. (2013). Ameliorative effect of Luffa acutangula Roxb. on doxorubicin induced cardiac and nephrotoxicity in mice. Indian J. Exp.
Biol. 51, 149-156.
Jayachandran, M., Wu, Z., Ganesan, K., Khalid, S., Chung, S.M., Xu, B. (2019). Isoquercetin upregulates antioxidant genes, suppresses inflammatory cytokines and regulates AMPK pathway in streptozotocin-induced diabetic rats. Chem. Biol. Interact. 303, 62-69.
Jayachandran, M., Zhang, T., Ganesan, K., Xu, B., Chung, S.S.M. (2018). Isoquercetin ameliorates hyperglycemia and regulates key enzymes of glucose metabolism via insulin signaling pathway in streptozotocin-induced diabetic rats. Eur. J. Pharmacol. 829, 112-120.
Jiang, Z.Y., Hunt, J.V., Wolff, S.P. (1992). Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. Anal. Biochem. 202, 384-
Jun-kai, L.,Chen Minghuang, Xie Jieming, Zhao Rong, Ye Xiaoming, Shi Xiaoli, Wang Ziren(2002). Purification and Characterization of Two Luffaculins, Ribosome-inactivating Proteins from Seeds of Luffa acutangula. Chinese J Biochem. Mol. Biol. 18, 609-613
Kumar, G., Banu, G.S., Kannan, V., Pandian, M.R. (2005). Antihepatotoxic effect of beta-carotene on paracetamol induced hepatic damage in rats. Indian J. Exp. Biol. 43, 351-355.
Kumar, G., Banu, G.S., Pandian, M.R. (2007). Biochemical activity of selenium and glutathione on country made liquor (CML) induced hepatic damage in rats. Indian J. Clin. Biochem. 22, 105-108.
Kumar, G., Banu, G.S., Pappa, P.V., Sundararajan, M., Pandian, M.R. (2004). Hepatoprotective activity of Trianthema portulacastrum L. against paracetamol and thioacetamide intoxication in albino rats. J.
Ethnopharmacol. 92, 37-40.
Kumar G, Murugesan.A.G. (2007). Influence of Helicteres isora bark extracts on plasma and tissue glycoprotein components in streptozotocin diabetic rats. J. Clin.
Diagn. Res. 1, 330-338.
Kumar, G., Murugesan, A.G. (2008). Hypolipidaemic activity of Helicteres isora L. bark extracts in streptozotocin induced diabetic rats. J.
Ethnopharmacol. 116, 161-166.
Kumar, G., Murugesan, A.G., Rajasekara Pandian, M. (2006). Effect of Helicteres isora bark extract on blood glucose and hepatic enzymes in experimental diabetes. Pharmazie 61, 353-355.
Kumar, G., Sharmila Banu, G., Murugesan, A.G. (2009). Attenuation of Helicteres isora L. bark extracts on streptozotocin-induced alterations in glycogen and carbohydrate metabolism in albino rats. Hum. Exp. Toxicol. 28,
-696.
Misar, A., Upadhye, A., Mujumdar, A. (2004). CNS depressant activity of ethanol extract of Luffa acutangula Var. amara C. B. Clarke. Fruits in mice. Indian J. Pharma. Sci. 66, 463-465.
Nagarajaiah, S.B., Prakash, J. (2015). Chemical Composition and Bioactive Potential of Dehydrated Peels of Benincasa hispida, Luffa acutangula, and Sechium edule. J. Herbs, Spices Med. Plants 21, 193 - 202.
Nallappan, D. (2016). Biogenic synthesis, characterization, and biological activity of silver nanoparticles from Eurycoma longifolia and Luffa acutangula.J. Chem. Pharma. Res.
Niehaus, W.G., Jr., Samuelsson, B. (1968). Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur. J.
Biochem. 6, 126-130.
Pimple, B.P., Kadam, P.V., Patil, M.J. (2012). Protective effect of Luffa acutangula extracts on gastric ulceration in NIDDM rats: role of gastric mucosal glycoproteins and antioxidants. Asian Pac. J. Trop. Med. 5, 610-615.
Quanico, J., Amor, E.C., Perez, G. (2008). Analgesic and hypoglycemic activities of Bixa orellana, Kyllinga monocephala and Luffa acutangula.
Philippine J. Sci. 137, 69-76.
Rahman, M. (2014). In-vitro Evaluation of Cytotoxic and Anthelmintic Activity of Luffa acutangula, Luffa aegyptiaca and Momordica cochinchinensis. Br.J.Pharma.Res. 4, 267-277.
Raj, S., Mohammed, S., Kumar, S.V., SanthoshKumar, C., Debnath, S. (2012). Antidiabetic Effect of Luffa acutangula fruits and histology of organs in streptozotocin induced diabetic in rats. Res. J. Pharmacogn.
Phytochem. 4, 64-69.
Ravin, H.A. (1961). An improved colorimetric enzymatic assay of ceruloplasmin. J. Lab. Clin. Med. 58, 161-168.
Roe, J.H., Kuether, C.A. (1943). The determination of ascorbic acid in whole blood and urine through the 2,4- dinitrophenylhydrazine derivative of dehydroascorbic acid. J. Biol. Chem. 147, 399-407.
Sharmila Banu, G., Kumar, G., Murugesan, A.G. (2009a). Effect of ethanolic leaf extract of Trianthema portulacastrum L. On aflatoxin induced hepatic damage in rats. Indian J. Clin.
Biochem. 24, 414-418.
Sharmila Banu, G., Kumar, G., Murugesan, A.G. (2009b). Ethanolic leaves extract of Trianthema portulacastrum L. ameliorates aflatoxin B(1) induced hepatic damage in rats. Indian J. Clin. Biochem. 24, 250-256.
Sharmila Banu G, Kumar, G., Rajasekara Pandian M.(2007). Cholesterol-Lowering Activity of the Aqueous Fruit Extract of Trichosanthes dioica Roxb (L.) in Normal and Streptozotocin Diabetic Rats. J. Clin. Diagn. Res. 1, 561-569.
Sharmin, R., Khan, M.I., Akhtar, M.A., Alim, A., Islam, M.A., Anisuzzaman, A.S.M., Ahmed, M. (2012). Hypoglycemic and Hypolipidemic Effects of Cucumber, White Pumpkin and Ridge Gourd in Alloxan Induced Diabetic Rats. J. Sci. Res. 5, 161-170.
Shendge, P.N., Belemkar, S.(2018). Therapeutic Potential of Luffa acutangula: A Review on Its Traditional Uses, Phytochemistry, Pharmacology and Toxicological Aspects. Front.
Pharmacol. 9, 1177.
Sukalingam, K., Ganesan, K. (2015). Rhesus Blood Groups Associated with Risk to Obesity and Diabetes Mellitus : A Report on Punjabi Population in Selangor, Malaysia. Int. J. Integr. Med. Sci. 2, 105-109.
Sukalingam, K., Ganesan, K., Ponnusamy, K. (2015). Pharmacological Properties of Trianthema portulacastrum L and its Therapeutic Potential as Complementary Medicine.
Int. J. Pharma. Chem. Sci. 4, 269-274. 62.Sukalingam, K., Ganesan, K., Xu, B. (2017). Trianthema portulacastrum L. (giant pigweed): phytochemistry and pharmacological properties. Phytochem. Rev. 16, 461-478.
Sukalingam, K., Ganesan, K., Xu, B. (2018). Protective Effect of Aqueous Extract from the Leaves of Justicia tranquebariesis against Thioacetamide
Induced Oxidative Stress and Hepatic Fibrosis in Rats. Antioxidants (Basel) 7, 78.
Sukalingam, K., Ganesan, K., Gani, S.B. (2013). Hypoglycemic Effect of 6- Gingerol, an Active Principle of Ginger in Streptozotocin Induced Diabetic Rats. Res. Rev: J. Pharmacol. Toxicol. Stud. 1, 33-37.
Sulaiman, S.F., Ooi, K.L., Supriatno, (2013). Antioxidant and α-glucosidase inhibitory activities of cucurbit fruit vegetables and identification of active and major constituents from phenolic
rich extracts of Lagenaria siceraria and Sechium edule. J. Agric. Food Chem. 61, 10080-10090.
Thatchinamoorthi, R., Ganesan, K., Pandian, M.R. (2021). Hypoglycemic effects of Luffa acutangula (L.) Roxb. fruit extract in normal and streptozotocin-induced diabetic rats. Int. J. Pharma. Sci. Res. 12, 2282-2288.
Trinder, P. (1969). Determination of blood glucose using an oxidase peroxidase system with a non carcinogenic chromogen. J. Clin. Pathol. 22, 158-161.
Ulaganathan, I., Divya, D., Radha, K., Vijayakumar, T., Dhanaraju, M. (2010). Protective effect of Luffa acutangula (var) amara against carbon tetrachloride-induced hepatotoxicity in experimental rats. Res. J. Biol. Sci. 5,
-624.
Vanajothi, R., Sudha, A., Manikandan, R., Rameshthangam, P., Srinivasan, P. (2012). Luffa acutangula and Lippia nodiflora leaf extract induces growth inhibitory effect through induction of apoptosis on human lung cancer cell line. Biomed. Prev. Nutr. 2, 287-293.
Xu, B., Ganesan, K., Mickymaray, S., Alfaiz, F.A., Thatchinamoorthi, R., Aboody, M.S.A. (2020). Immunomodulatory and antineoplastic efficacy of common spices and their connection with phenolic antioxidants.Bioactive Comp Health
Dis. 3, 15-31.
Zhang, T., Jayachandran, M., Ganesan, K., Xu, B. (2018). Black Truffle Aqueous Extract Attenuates Oxidative Stress and Inflammation in STZ-Induced Hyperglycemic Rats via Nrf2 and NF-κB Pathways. Front. Pharmacol. 9, 1257.
Zhang, T., Jayachandran, M., Ganesan, K., Xu, B. (2020). The Black Truffle, Tuber melanosporum (Ascomycetes), Ameliorates Hyperglycemia and Regulates Insulin Signaling Pathway in STZ-Induced Diabetic Rats. Int. J. Med.
Mushrooms 22, 1057-1066.
