Electrophoretic Banding Patterns of Esterase Isozymes in Fresh Water Fish Channa punctatus

Authors

  • M Venkateswara Rao Assistant Professor, Dept. of Zoology, SR & BGNR Degree College, Khammam, Telangana 5070
  • Y Venkaiah Associate Professor of Zoology, Department of Zoology, Kakatiya University, Warangal, Telangana State 506009, India

DOI:

https://doi.org/10.48165/

Keywords:

α-naphthylacetate, Channa punctatus, Electrophoretic banding patterns, Esterase

Abstract

Esterase enzymes catalyse the formation and  breakdown of carboxylic acid esters of alcohols. The  present work aimed to study the electrophoretic  banding patterns of esterase isozymes in fresh water  fish Channa punctatus The results revealed that the  electrophoretic esterase banding patterns varied in  different tissues i.e. gill, liver, intestine, muscle, and  brain of fish c. puctatus Esterase patterns were  separated on thin layer 1.5mm (thickness)  polyacrylamide gels (SDS-7.5٪) and stained with α naphthyl acetate used as substrate. Three different  esterase bands were detected and named as Esi-1; Est 2; and Est-3; with different relative mobilities such as  0.6 ±0.05; 0.4±0.05; 0.3±0.05. All the three esterase  bands were found in all tissues i.e. gill, liver, intestine,  muscle and brain. Among the three esterases Est-1 is  found in all the tissues. Est-2and Est-3 were found in  all the tissues. Studieson esterases of fishes and other organisms revealed similar type of patterns of esterase  were noticed in one or the other tissue of all the animals. 

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References

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Published

2022-06-15

Issue

Vol. 41 No. 1 (2022): Bulletin of Pure and Applied Sciences-Zoology (Jan-Jun) 2022

Section

Articles

How to Cite

Electrophoretic Banding Patterns of Esterase Isozymes in Fresh Water Fish Channa punctatus . (2022). Bulletin of Pure & Applied Sciences- Zoology , 41(1), 34–39. https://doi.org/10.48165/