Electrophoretic Banding Patterns of Esterase Isozymes in Fresh Water Fish Channa punctatus
DOI:
https://doi.org/10.48165/Keywords:
α-naphthylacetate, Channa punctatus, Electrophoretic banding patterns, EsteraseAbstract
Esterase enzymes catalyse the formation and breakdown of carboxylic acid esters of alcohols. The present work aimed to study the electrophoretic banding patterns of esterase isozymes in fresh water fish Channa punctatus The results revealed that the electrophoretic esterase banding patterns varied in different tissues i.e. gill, liver, intestine, muscle, and brain of fish c. puctatus Esterase patterns were separated on thin layer 1.5mm (thickness) polyacrylamide gels (SDS-7.5٪) and stained with α naphthyl acetate used as substrate. Three different esterase bands were detected and named as Esi-1; Est 2; and Est-3; with different relative mobilities such as 0.6 ±0.05; 0.4±0.05; 0.3±0.05. All the three esterase bands were found in all tissues i.e. gill, liver, intestine, muscle and brain. Among the three esterases Est-1 is found in all the tissues. Est-2and Est-3 were found in all the tissues. Studieson esterases of fishes and other organisms revealed similar type of patterns of esterase were noticed in one or the other tissue of all the animals.
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