Effect of Malathion (An Organophospahte) on Electrophoretic Banding Patterns of Esterase Isozymes in Gill, Liver, Brain Tissue of Fresh Water Fish Channa Punctatus (Bloch)

Authors

  • Venkateswara Rao Mandalapu Department of Zoology, SR and BGNR Government Arts and Science College, Khammam, Telanga
  • Venkaiah Yanamala Department of Zoology, Kakatiya University, Warangal, Telangana State Telangana 506009, India

DOI:

https://doi.org/10.48165/bpas.2023.42A.1.12

Keywords:

Electrophoretic banding patterns, Esterase Isozymes, Channa punctatus, α-naphthyl acetate, PAGE, Malathion (an Organophosphate), different time intervals

Abstract

This investigation was conducted to compare the  electrophoretetic banding patterns of esterase isozyme  in the gill, liver, and brain tissues of the freshwater fish  Channa punctatus (Bloch) at 24 hours, 48 hours, 72  hours, and 96 hours after exposure to Malathion (an  Organophosphate) to those at the control group.  Quantitative analysis of the esterase isozymes was  performed using 7.5 native polyacrylamide gel  electrophoresis (PAGE) stained with -naphthyl acetate  as substrate. The relative mobilities of three esterase  Isozyme bands in gill, liver, and brain tissue were  determined to be 0.60.05, 0.40.05, and 0.30.05,  respectively; these bands were designated Est-1, Est-2,  and Est-3, respectively. Control samples from the gill, liver, and brain all had all three esterase bands. Both Est 2 and Est-3 Esterase Isozyme bands in gill and liver  tissue were eliminated when fish were subjected to  Malathion (an Organophosphate) for 72 and 96 hours,  respectively. Malathion induced greater damage in Est-1  and Est-3 brain tissue than in Est-2. 

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Published

2023-06-16

Issue

Vol. 42 No. 1 (2023): Bulletin of Pure and Applied Sciences-Zoology (Jan-Jun) 2023

Section

Articles

How to Cite

Effect of Malathion (An Organophospahte) on Electrophoretic Banding Patterns of Esterase Isozymes in Gill, Liver, Brain Tissue of Fresh Water Fish Channa Punctatus (Bloch) . (2023). Bulletin of Pure & Applied Sciences- Zoology , 42(1), 140–148. https://doi.org/10.48165/bpas.2023.42A.1.12