Insulin resistance and Oxidant-Antioxidant Markers in Young Women with Polycystic Ovarian Syndrome

Authors

  • K Lakshmi Ph. D Research scholar, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysuru, Karnataka 570006, India.
  • Suttur S Malini -mail: laksh.k.nayak666@gmail.com 2Professor and Chairperson, Department of Studies in Genetics and Genomics, University of Mysore, Manasagangothri, Mysuru, Karnataka 570006, India.

DOI:

https://doi.org/10.48165/bpas.2023.42A.1.9

Keywords:

Infertility, Insulin resistance, Oxidative stres, Polycystic ovary syndrome, Antioxidants

Abstract

Introduction: Oxidative stress and insulin resistance (IR) may  contribute to the pathogenesis of polycystic ovarian syndrome  (PCOS). IR promotes hyperglycaemia, which may increase the  production of reactive oxygen species and decrease in  antioxidant levels in PCOS. Aim: To investigate the oxidant-antioxidant status and its  association with insulin resistance in young women with  PCOS.Materials and methods: The present study involved two  groups, PCOS (n = 55) and age and BMI matched controls (n =  55). Serum levels of various oxidative stress markers  malondialdehyde (MDA), antioxidants- superoxide dismutase  (SOD), catalase, and total antioxidant capacity (TAC) were  measured using spectrophotometric assays. HOMA-IR method  was used to assess the insulin resistance. Results were  analysed to compare and correlate insulin resistance with these  oxidative stress markers. Results: PCOS group had significantly higher MDA and  lower TAC activity, SOD, catalase levels than the control  group (p < 0.001). PCOS patients with IR had significantly  higher MDA, while SOD, catalase, TAC levels lower than the  PCOS patients without IR (p< 0.001). Infertile PCOS patients  had significantly higher MDA, and lower SOD, catalase, TAC  level than the fertile PCOS patients (p<0.001). There was a  statistically significant and positive correlation between  HOMA-IR and MDA levels, whereas HOMA-IR showed  statistically significant negative correlation with SOD, catalase  and TAC levels. onclusion: Our findings suggest that young PCOS women  may experience oxidative stress due to insulin resistance. As a  result, the existence of insulin resistance, hyperinsulinemia,  and oxidative damage in PCOS is likely to hasten the gradual  progression of cardiovascular disease. 

Downloads

Download data is not yet available.

References

Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 121–126.

Agarwal, A., Aponte-Mellado, A., Premkumar, B. J., Shaman, A., & Gupta, S. (2012). The effects of OS on female reproduction: a review. Reproductive Biology and Endocrinology, 10(1), 49.

Agarwal, A., Gupta, S., & Sharma, R. K. (2005). Role of OS in female reproduction. Reproductive Biology and Endocrinology, 3(1).

Al-Gubory, K. H., Fowler, P. A., & Garrel, C. (2010). The roles of cellular reactive oxygen species, OS and antioxidants in pregnancy outcomes. The International Journal of Biochemistry & Cell Biology, 42(10), 1634– 1650.

Bernheim, F., Bernheim, M. L., & Wilbur, K. M. (1948). The reaction between thiobarbituric acid and the oxidation products of certain lipides. Journal of Biological Chemistry, 174(1), 257–264.

Bloch-Damti, A., & Bashan, N. (2005). Proposed Mechanisms for the Induction of Insulin Resistance by OS. Antioxidants & Redox Signaling, 7(11–12), 1553–1567. https://doi.org/10.1089/ars.2005.7.1553

Boomsma, C., Eijkemans, M., Hughes, E., Visser, G., Fauser, B., & Macklon, N. (2006). A meta-analysis of pregnancy outcomes in women with polycystic ovary syndrome. Human Reproduction Update, 12(6), 673–683.

Das, S., Chattopadhyay, R., Ghosh, S., Ghosh, S., Goswami, S. K., Chakravarty, B. N., & Chaudhury, K. (2006). Reactive oxygen species level in follicular fluid-- embryo quality marker in IVF?. Human reproduction (Oxford, England), 21(9), 2403–

Desai, V., Prasad, N. R., Manohar, S. M., Sachan, A., Narasimha, S. R. P. V. L., &Bitla, A. R. R. (2014). OS in non-obese women with polycystic ovarian syndrome. Journal of clinical and diagnostic research, 8(7), CC01.

ESHRE, T. R., & ASRM-Sponsored PCOS Consensus Workshop Group. (2004). Revised 2003 consensus on diagnostic

criteria and long-term health risks related to polycystic ovary syndrome. Fertility and sterility, 81(1), 19-25

Fang, Y. Z., Yang, S., & Wu, G. (2002). Free radicals, antioxidants, and nutrition. Nutrition, 18(10), 872–879.

Gao, D., Nong, S., Huang, X., Lu, Y., Zhao, H., Lin, Y., & Li, J. (2010). The effects of palmitate on hepatic insulin resistance are mediated by NADPH Oxidase 3-derived reactive oxygen species through JNK and p38MAPK pathways. Journal of Biological Chemistry, 285(39), 29965-29973.

Kazari D. (2000). A modified spectrophotometric assay of superoxide dismutase using nitrate formation by superoxide radical. Indian Journal of Biochemistry and Biophysics, 57, 201-4

Kuşçu, N. K., &Var, A. (2009). OS but not endothelial dysfunction exists in non-obese, young group of patients with polycystic ovary syndrome. Acta Obstetricia et Gynecologica Scandinavica, 88(5), 612–617.

Legro, R. S., Castracane, V. D., & Kauffman, R. P. (2004). Detecting Insulin Resistance in Polycystic Ovary Syndrome: Purposes and Pitfalls. Obstetrical & Gynecological Survey, 59(2), 141–154.

Masharani, U. B., Maddux, B. A., Li, X., Sakkas, G. K., Mulligan, K., Schambelan, M., & Youngren, J. F. (2011). Insulin resistance in non-obese subjects is associated with activation of the JNK pathway and impaired insulin signaling in skeletal muscle. PloS one, 6(5), e19878.

Nuñez-Calonge, R., Cortés, S., Gutierrez Gonzalez, L. M., Kireev, R., Vara, E., Ortega, L., Caballero, P., Rancan, L., &Tresguerres, J. (2016). OS in follicular fluid of young women with low response compared with fertile oocyte donors. Reproductive biomedicine online, 32(4), 446–456.

Ovalle, F., & Azziz, R. (2002). Insulin resistance, polycystic ovary syndrome, and type 2 diabetes mellitus. Fertility and Sterility, 77(6), 1095-105.

Özer, A., Bakacak, M., Kıran, H., Ercan, Ö.,Köstü, B., Kanat-Pektaş, M., Kılınç, M., & Aslan, F. (2016). Increased OS is associated with insulin resistance and infertility in

polycystic ovary syndrome. Ginekologiapolska, 87(11), 733–738.

Poretsky, L., Cataldo, N. A., Rosenwaks, Z., &Giudice, L. C. (1999). The Insulin-Related Ovarian Regulatory System in Health and Disease. Endocrine Reviews, 20(4), 535–582.

Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Analytical Biochemistry, 269(2), 337–341.

Sabuncu, T., Vural, H., Harma, M., & Harma, M. (2001). OS in polycystic ovary syndrome and its contribution to the risk of cardiovascular disease. Clinical biochemistry, 34(5), 407–413.

Schieber, M., & Chandel, N. (2014). ROS Function in Redox Signaling and OS. Current Biology, 24(10), 453-62.

Seow, K. M., Juan, C. C., Wu, L. Y., Hsu, Y. P., Yang, W. M., Tsai, Y. L., Hwang, J. L., &Ho, L. T. (2004). Serum and adipocyte resistin in polycystic ovary syndrome with insulin resistance. Human reproduction (Oxford, England), 19(1), 48–53.

Singh, A. K., Chattopadhyay, R., Chakravarty, B., & Chaudhury, K. (2013). Markers of OS in follicular fluid of women with endometriosis and tubal infertility undergoing IVF. Reproductive toxicology (Elmsford, N.Y.), 42, 116–124.

Sulaiman, M., Al-Farsi, Y., Al-Khaduri, M., Saleh, J., & Waly, M. (2018). Polycystic ovarian syndrome is linked to increase OS in Omani women. International Journal of Women’s Health, Volume 10, 763–771.

Takeda, E., Arai, H., Yamamoto, H., Okumura, H., & Taketani, Y. (2005). Control

of OS and metabolic homeostasis by the suppression of postprandial hyperglycemia. The Journal of Medical Investigation, 52(Supplement), 259-265.

Talbott, E. O., Guzick, D. S., Sutton-Tyrrell, K., McHugh-Pemu, K. P., Zborowski, J. V., Remsberg, K. E., & Kuller, L. H. (2000). Evidence for Association Between Polycystic Ovary Syndrome and Premature Carotid Atherosclerosis in Middle-Aged Women. Arteriosclerosis, Thrombosis, and Vascular Biology, 20(11), 2414-21.

Turan, V., Sezer, E. D., Zeybek, B., & Sendag, F. (2015). Infertility and the Presence of Insulin Resistance Are Associated With Increased OS in Young, Non-obese Turkish Women With Polycystic Ovary Syndrome. Journal of Pediatric and Adolescent Gynecology, 28(2), 119–123.

Xiang, S. K., Hua, F., Tang, Y., Jiang, X. H., Zhuang, Q., & Qian, F. J. (2012). Relationship between Serum Lipoprotein Ratios and Insulin Resistance in Polycystic Ovary Syndrome. International Journal of Endocrinology, 2012, 1–4.

Yeon Lee, J., Baw, C. K., Gupta, S., Aziz, N., & Agarwal, A. (2010). Role of OS in Polycystic Ovary Syndrome. Current Women’s Health Reviews, 6(2), 96–107.

Zhao, H., Zhang, J., Cheng, X., Nie, X., & He, B. (2023). Insulin resistance in polycystic ovary syndrome across various tissues: an updated review of pathogenesis, evaluation, and treatment. Journal of Ovarian Research, 16(1).

Zuo, T., Zhu, M., & Xu, W. (2016). Roles of OS in Polycystic Ovary Syndrome and Cancers. Oxidative Medicine and Cellular Longevity, 2016, 1–14.

Downloads

Published

2023-06-16

Issue

Vol. 42 No. 1 (2023): Bulletin of Pure and Applied Sciences-Zoology (Jan-Jun) 2023

Section

Articles

How to Cite

Insulin resistance and Oxidant-Antioxidant Markers in Young Women with Polycystic Ovarian Syndrome . (2023). Bulletin of Pure & Applied Sciences- Zoology , 42(1), 106–116. https://doi.org/10.48165/bpas.2023.42A.1.9