Validated HPTLC method for simultaneous estimation of metoprolol succinate and ramipril in bulk drug and marketed formulation

Abstract

This paper describes a new, simple, precise, and  accurate HPTLC method for simultaneous  estimation of Metoprolol succinate and Ramipril  as the bulk drug and in tablet dosage forms.  Chromatographic separation of the drugs was  performed on aluminum plates precoated with  silica gel 60 F254 as the stationary phase and the  solvent system consisted of Methanol: Toluene:  Ethyl Acetate: Ammonia (2.5:3:5:0.7v/v/v/v).  Densitometric evaluation of the separated zones  was performed at 209 nm. The two drugs were  satisfactorily resolved with RF values 0.67, and  0.37 for Metoprolol Succinate and Ramipril,  respectively. The accuracy and reliability of the  method was assessed by evaluation of linearity  (2000-12000 ng/spot for Metoprolol succinate  and 200–1200 ng/spot for Ramipril), precision  (intra-day RSD 0.471–1.036% and inter-day RSD  1.085–1.580% for Metoprolol Succinate and intra day RSD 1.057–1.63% and inter-day RSD 1.024– 1.746% for Ramipril), accuracy (98.95 ± 0.16 % for  Metoprolol and 98.98 ± 0.41 % for Ramipril), and  specificity, in accordance with ICH guidelines.