Antimicrobial Activities of Methanol Leaf Extract of Carissa edulis Vahl (Apocynaceae)

Authors

  • I S Dawa Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, P.M.B 1069, Maiduguri, Borno State, Nigeria.
  • J Yakubu Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, P.M.B 1069, Maiduguri, Borno State, Nigeria.
  • U T Mamza Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, P.M.B 1069, Maiduguri, Borno State, Nigeria.
  • V M Balami Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, P.M.B 1069, Maiduguri, Borno State, Nigeria.
  • O A Sodipo Department of Clinical Pharmacology and Therapeutics, Faculty of Basic Clinical Science, College of Medical Sciences, University of Maiduguri, P.M.B 1069, Maiduguri, Borno State, Nigeria.
  • F I Abdulrahman Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, P.M.B 1069, Maiduguri, Borno State, Nigeria.
  • I Z Khan Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, P.M.B 1069, Maiduguri, Borno State, Nigeria

DOI:

https://doi.org/10.48165/

Keywords:

Carissa edulis, Antimicrobial, in vitro, Microorganism

Abstract

The research aims at evaluating in-vitro antimicrobial effects of methanolic leaf extract of Carissa  edulis. The leaf of Carissa edulis was collected air-dried, ground to powder and extracted with  methanol by cold extraction method to give a percentage yield of 28.03% w/w. The crude extract was  defatted with n-hexane and partitioned with chloroform, n-butanol and water to give a percentage  yield of 36.04%, 11.24%, 15.94% and 3.08% respectively. The extract fractions exhibited considerable  amount of inhibition against almost all the test micro-organisms except for the n-hexane extract which  had no activity. The activity of n-butanol extract (500 mg/ml), chloroform extract (500 mg/ml) and  aqueous extract (500 mg/ml) was superior to the standard drug (Gentamacin) used. The MIC and  MBC against the tested organisms ranged from 25 mg/ml to 50 mg/ml and 50 mg/ml to 100 mg/ml  respectively. The results obtained from this study revealed that the leaf extract of Carissa edulis possesses antibacterial activity against some pathogenic microorganisms such as Escherichia coli,  Klebsiella pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus. 

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Published

2021-12-15

Issue

Vol. 40 No. 2 (2021): Bulletin of Pure and Applied Sciences Chemistry (Jul-Dec) 2021

Section

Articles

How to Cite

Antimicrobial Activities of Methanol Leaf Extract of Carissa edulis Vahl (Apocynaceae) . (2021). Bulletin of Pure and Applied Sciences-Chemistry , 40(2), 96–103. https://doi.org/10.48165/