Nutritional Content, Antimicrobial Study and Isolation of Quercetin from Ziziphus spina-Christi Leaf

Authors

  • I Z Khan Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, Borno State, Nigeria
  • J Yakubu Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, Borno State, Nigeria
  • U T Mamza Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, Borno State, Nigeria
  • I S Khan Suny Downstate Health Sciences University, Department of Pediatrics Children’s Hospital New York, USA.
  • M Shah Parsons 10625 Rd, Johns Creek, GA 30097 Atlanta, USA.
  • M Arshad Study Hall, Vipul Khand, Gomti, Nagar, Lucknow, Uttar Pradesh 226010, India
  • L Markus Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, Borno State, Nigeria
  • B Moses Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, Borno State, Nigeria

DOI:

https://doi.org/10.48165/bpas.2023.42C.2.2

Keywords:

Ziziphus spina-Christi, Phytochemical, Quercetin, Antimicrobial, Elements

Abstract

Plant has been the major source of medicinal globally. This study aimed at determining the nutritional  composition, antimicrobial activity and isolation of phytochemical from the leaf of Ziziphus spina-Christi.  Proximate composition, elemental (Ca, Na, Mg, K, P, Cu, Cd, Fe, Ni, Zn) concentrations, phytochemical  screening and antimicrobial activity were carried out using standard protocols. Column chromatographic  elution process was used to fractionate the methanol extract and Preparative TLC was used to isolate a  compound. The result of the proximate composition and elemental concentration levels showed that the  leaf is nutritious. Phytochemical screening showed the presence of alkaloids, cardiac glycosides,  flavonoids, saponins and tannins. The methanol extract inhibited the growth of Escherichia coli, Salmonella  typhi, Staphyloccocus aureus and Bacillus subtilis significantly at extract concentrations of 100, 200, 300, 400  and 500mg/mL was used, thus having antimicrobial activity. The compound isolated has 114-116oC  melting point (uncorrected) with a single banding pattern confirmed by TLC study. Physical and  chemical tests of isolated compound were conducted. By using IR,1H-NMR and 13C-NMR spectra of the  isolated compound was confirmed as Quercetin – a flavonoid.  

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Published

2023-12-23

Issue

Vol. 42 No. 2 (2023): Bulletin of Pure and Applied Sciences-Chemistry (Jul-Dec) 2023

Section

Articles

How to Cite

Nutritional Content, Antimicrobial Study and Isolation of Quercetin from Ziziphus spina-Christi Leaf . (2023). Bulletin of Pure and Applied Sciences-Chemistry , 42(2), 57–69. https://doi.org/10.48165/bpas.2023.42C.2.2