Effect of Zeatin and Indole-3-Acetic Acid in Vitro Culture of Butea  Buteiformis Voigt

Belai Meeta Suwal  Singh

doi:10.48165/

Authors

Belai Meeta Suwal Singh Central Department of Botany, Patan Multiple Campus Patan Lalitpur, Patan 44700, Nepal

DOI:

https://doi.org/10.48165/

Keywords:

Micropropagation, Murashige & Skoog (1962) medium, Nodal explants, sand rooting, Acclimatization

Abstract

Butea buteiformis is a valuable tree with large trifoliate leaves with beautiful flower. The seeds were surface sterilized and cultured on half strength Murashige & Skoog (1962) (MS) medium. Nodal explants obtained from cultured were subcultured on different concentrations of zeatin (Zin) and Indole-3-acetic acid (IAA). The best proliferation of nodes and shoots were observed on the MS medium supplemented with 5 µM Zin and 1.0 M IAA. After 8 weeks of culture the propagated plants were acclimatized and transferred to the sand box containing 1:1 soil and sand. Well rooted plants were then established in the field. All the data collected were worked out statistically with SPSS, a system of analytical procedure.

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