Comparative Taxonomic study on Crotalaria L. from some parts of  South-Eastern Nigeria

C Ekeke; J U Agogbua

doi:10.48165/

Authors

C Ekeke Department of Plant Science and Biotechnology, Faculty of Science University of Port Harcourt, Nigeria
J U Agogbua Department of Plant Science and Biotechnology, Faculty of Science University of Port Harcourt, Nigeria

DOI:

https://doi.org/10.48165/

Keywords:

Crotalaria, Petiole, Anatomy, Trichome density

Abstract

Morphological and anatomicalcharacteristics of stem, root, petiole, midrib, and lamina of Crotalaria L. found in some parts South-Eastern Nigeria were investigated using microtomy and visual observation to provide additional information to delimit them. Anisocytic, paracytic, anomocytic, tetracytic, one-subsidiary cell and contiguous stomata were recorded among the species. anisocytic was the predominant stomata in the three Crotalaria species studied occurring on the adaxaial and the abaxial leaf surfaces. Tetracytic stomata were observed on the abaxial surfaces of C. goreensis and C. verrucosa but not seen on the leaf surface of C. retusa. Also, contuigous stomata were observed on the abaxial leaf surface of C. goreensis and adaxial surface of C. retusa. Furthermore, stomata in groups of 2 to 3 were recorded on the adaxial surfaces of C. retusa and C. goreensis. On the other hand, stomata with one-subsidiary cell was recorded on the abaxial surface of C. retusa and adaxial surface of C. verrucosa.The average trichome length include 36.58 µm in C. retusa, 56.62 µm in C. retusa and 61.15 µm in C. verrucosa. The hairiness or trichome density among the species varied C. verrucosa (20 – 23 per 100 objective fields of view), C. goreensis (40 – 45 OFV), and C. ratusa (76 – 100 OFV). A combination of morphological and anatomical of petiole, midrib, stem anatomy, and epidermal characters (stomata types, trichome size, and density) varied among the species studied and are diagnostic especially when combined the morphological data.

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References

Akintayo, E. T. (1997). Chemical composition phytochemical properties of fluted pumpkin (Teifariaoccidentalis) seed and seed oil.R. Vista Italian sostanze Grasse, 74: 13-16. 2. Akobundu, I. O., Ekeleme, F., Agyakwa, C.W. and Ogazie, C.A. (2016). A handbook of West African weeds.International Institute of Tropical Agriculture, Ibadan, Nigeria.Pp.1 –381. 3. Ansari, A.A.(2008). Crotalaria L. in India. Dehra-Dunn: Bishen Singh Mahendra Pal Singh, 376p.

APG, 2012; APG (2012). Angiosperm Phylogeny Group. Fabales.

[www.mobot.org/mobot/research/APweb/orders/fabales web.htm]. Date accessed: 5/9/2014.

Arvinder K. N. (1977). Anatomical studies in Crotalaria and Tephrosiaspecies.Vasculature of flower.Proceedings of the Indian Academy of Sciences – Section B. 6: 361–369.

Çildir, H., Kahraman, A., Doğan, M. and Büyükkartal, H. N. (2017). Comparative Anatomical and Micromorphological Studies on Some Species of Lathyrus L. Section Lathyrus (Papilionoideae, Fabaceae). Comm. J. Biol. 1 (1):42-50.

Cook, C. G. and White, G. A. (1996). Crotalaria juices: A potential multipurpose fiber crop in J. Janic (ed). Fast and accurate phylogeny reconstruction alogorithms based on the minimum-evolution principle. J. Computation Biol. 9:687-705.

Dilcher, D. L. (1974).Approaches to the identification of angiosperm leaf remains.Bot. Rev 40 1-157. https://doi.org 10 .1007/BF02860067.

Duane I. and Paul E. B. (2012).Origin of Fabales and its relationship with other plant families.Encyclopaedia Britinnica.Inc.,

Heuzé, V. Thiollet, H., Tran, G. and Lebas, F. (2018). Sunn hemp (Crotalaria juncea) Feedipedia, a programme by INRA, CIRAD, AFZ and FAO.https://www.feedipedia.org/ node/313 Last updated on November 27, 2018, 11:46.

Hutchinson, J. and Dalziel J.M. (1954). Flora of West Tropical Africa. Revised by Keay , R. W. J. Vol. I Part I. Crown Agents for Oversea Governments and Administrations, London. 12. Keener, B. R., Diamond, A.R., Davenport, L. J., Davison, P. G., Ginzbarg,, S. L., Hansen, C. J.

Major, C. S., Spaulding, D. D., Triplett, J. K. and Woods, M. (2018). Alabama Plant Atlas.[S.M. Landry and K.N. Campbell (original application development), Florida Center for Community Design and Research. University of South Florida]. University of West Alabama, Livingston, Alabama.

Marcelo F. D., Jose R. P. and Gladys F. D. M. (2014). Comparative leaf anatomy and morphology of some Brazilian species of Crotalaria L. (Leguminosae: Papilionoideae: Crotalarieae). ActaBotanicaBrasilica, 28(4): 583-593.

Marianne, M. L., Ben‐Erik, V., James, S. B. and Patricia, M. T. (2010). The systematic significance of morphological and anatomical variation in fruits of Crotalaria and related genera of tribe Crotalarieae (Fabaceae) Botanical Journal of Linn. Society, 165(1): 84-106. https://doi.org/10.1111/j.1095-8339.2010.01098.x.

Massoud R. and Zahra H. (2016). Comparative leaf epidermis and anatomical study in populations of Trigonellaspruneriana (Fabaceae) from Iran.Journal of Plant Taxonomy and Geography. 71(1): 107-115 e 1. https://doi.org/10.1080/00837792.2016.1138673

Metcalfe C.R., and Chalk L. 1972. Anatomy of the Dicotyledons 2, 1041–1053. Oxford University Press, Oxford.

Metcalfe, C.R. and Chalk, L. (1979). Anatomy of the Dicotyledons: Systematic Anatomy of Leaf and Stem, with a Brief History of the Subject. 2nd Edn., Clarendon Press, Oxford, ISBN: 9780198543831, Pages: 304.

Mukurasi, N.J. (1986). Agricultural attributes of Crotalaria zanzibaries, UgoleAgric Centre, Mbeya 6 Tanzania. Journal of Agricultural Research 47(2): 617-625.

Nagar, N. and Albert, S. (2013). An unusual multicarpellary condition in Crotalaria verrucosa L. (Fabaceae) from Gujarat (India).Journal of Plant Taxonomy and Geography, 68(2): 187–190, http://dx.doi.org/10.1080/00837792.2013.840050.

Ndukwu, B. C. and Okoli, B. E. (1992). Studies on Nigerian Curcurbitamoschata.Nigerian Journal of Botany 5:18-26.

Nuhu, H., Abdurrahman, E. M., Shok, M. (2009). Ethnomedical studies of Crotalaria species found in Zaria, northern Nigeria. Nigerian Journal of Pharmaceutical Sciences, 8(2): 46-53. 22. Nuhu, H., Shock, M., Abdurahman, E.M. and Ibrahim, N.D.G. (2000). Alkaloid composition and toxicity studies of three Nigerian Crotalariaspecies.Nigeria Journal of Natural Products and Medicine, 4(1): 43-45.

Nwude, N. and Ibrahim M. A. (1980). Plants used in traditional veterinary practice in NigeriAJournal of veterinary Pharmacology and Therapeutics 72(12): 1294-1296.

Odewo, S. A., Adeyemo, A. and Ayodele, A. E. (2018). Foliar epidermal studies of the genus Crotalaria in Nigeria.Journal of Medicinal Plants Studies, 6(6): 245-249.

Odewo, S.A., Ajani, B. A., Soyewo. L. T, and Omiyale, O. A. (2015). Ecological Distribution of the Genus Crotalaria in Nigeria.International Journal of Scientific and Technology Research. 4(8): 348-355

Okeke, S.I., Edeoga, H.O., Nduche, M.U, Omosun, G (2019). Anatomical Studies on the Roots of Some Crotalaria L. Species (Fabaceae-Papilionoideae).International Journal of Research in Pharmacy and Biosciences, 6(3): 22-26.

Olowokudejo, J.D. (1990). Comparative morphology of leaf epidermis in the genus Annona (Annonaceae) inWest Africa.Phytomorph., 4 (3&4): 407 422.

Owolabi J. A. and Adedeji, O. (2018).Micromorphological study of some species of Papilionoideae from Nigeria.International Journal of Current Advanced Research, 7(2):9784- 9794. DOI: http://dx.doi.org/10.24327/ijcar.2018. 9794.1632.

Parveen, S. N., Murthy, K. S. R. and Pullaiah, T. (2000). Leaf epidermal characters in Crotalaria species (Papilionoideae) from Eastern Ghats.Phytomorphology, 50(2): 205-212. 30. Pullaiah, T. and Chandrasekhar-Naidu, K. (2003). Antidiabetic Plants in India and Herbal Based

Antidiabetic Research.Regency Publications, University of Michigan, ISBN: 8187498676, 321 pages

Roeder E, Wiedenfeld, H. (2009). Pyrrolizidine alkaloids in medicinal plants of Mongolia, Nepal and Tibev.In paharmazie. 64(11):699-716.

Samba RT, Sylla SN, Neyra M, Eueye M, Dreyfus B, Ndoye I. (2002). Biological Nitrogen Fixation in Crotalaria Species estimated using the 15 isotype dilution method. African Journal of Biotechnology. 1:17-22.

Shah, G. L. andGopal, B. V. (1969). Ontogeny of Stomata on the Foliar and Floral Organs of some Species of Crotalaria L.Annals of BotanyNew Series, 33(131): 553-560. https://www.jstor.org/stable/42908745.

Shankar, D. (2002). Sustainable Harvesting, Conservation, Cultivation and Marketing Linkages for Medicinal Plants of Andhra Pradesh Community Forest Management Project, Foundation for Revitalization of Local Health Traditions.

Sibichen M. T. (2004). “Morphologic and Taxonomic Studies on Fabaceae (Tribe: Crotalarieae) in South India” Thesis. Department of Botany, St. Joseph's College Kozhikode, University of Calicut.

Sirhar, H. B., Hoffer, J. A., Wolfgang, H. A. (2007). Comparative pharmacological and phytochemical analysis of in-vivo and in-vitro propagated Crotalaria species. Asian Pac. J Trop. Med. 5(1):37-41.

Sonje, S. B. and Bhuktar, A. S. (2013). Anatomical studies of Crotalaria albidaHeyne Ex Roth. Science Research Reporter, 3(2):155-158.

Thomas, E. (2003). For the love of insects.Belknap Press, ISBN 978-0-674-01827-3. 324. 39. Vardhana, R. (2008). Direct uses of medicinal plants and their identification. New Delhi: Sarup and Sons publication.

Wollenwebber, E. andSchnesder, H. (2000). Lipophilic exudates of Pteridaceae; chemistry and chemotaxonomy. Bio chem. Syst. Ecol. 28(8):751-777.