Genome Engineering in Livestock: Recent Advances and Regulatory Framework

Dharmendra Kumar; Wilfried A.  Kues

doi:10.48165/aru.2023.3.1.5

Authors

Dharmendra Kumar Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar-125001, Haryana, India
Wilfried A. Kues Friedrich-Loeffler-Institut, Federal Research Institute of Animal Health, Institute of Farm Animal Genetics, Depart ment of Biotechnology, Stem Cell Physiology, Höltystr 10, 31535 Neustadt, Germany

DOI:

https://doi.org/10.48165/aru.2023.3.1.5

Keywords:

Genome editing, CRISPR/Cas9, livestock, regulation, transgenesis

Abstract

Since the domestication of animals, livestock species are an important source of protein-rich food, and other animal products. The very recent progress in genetic engineering allows to modify the genomes of livestock species in an unprecedented way to improve productions traits, disease resistance, adaptation to climate changes, animal welfare aspects, but also to develop large animal models for developmental biology and biomedicine. Here, we concisely summarize the recent progress of genome-editing technologies, with a particular focus on the CRISPR/Cas9 designer nuclease, in livestock. Currently, precision-modified livestock lines with disease resistance and production traits are ready to be introduced into the commercial production. On a scientific basis these lines are considered safe for human consumption, especially for genome edits implementing only a single nucleotide change, which mimic ´natural´ point mutations. Internationally, however, there are clear differences in the interpretation of the legal framework on whether genome edited animals or their products need to be regulated.

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