Semen Sexing in Bovine: Current Status and the Need to Develop Alternative Techniques

Himani Joshi; Manya Mathur; AK Mohanty; Sudarshan  Kumar; JK Kaushik; TK Mohanty; Dharmendra Kumar; Sumit Kumar  Singh; Vanya Bhushan; Abhishek Parashar; Preeti Bhardwaj; Vinod Kumar  Yata

doi:10.48165/aru.2021.1203

Authors

Himani Joshi Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Manya Mathur Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
AK Mohanty Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Sudarshan Kumar Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
JK Kaushik Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
TK Mohanty Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Dharmendra Kumar Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Sumit Kumar Singh Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Vanya Bhushan Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Abhishek Parashar Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Preeti Bhardwaj Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Vinod Kumar Yata Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India

DOI:

https://doi.org/10.48165/aru.2021.1203

Keywords:

Semen sexing, Sex sorted semen, X and Y sperm, DNA

Abstract

Livestock industry is one of the important pillars for country economy. Sperm sexing has the potential of significantly improving the quality and quantity of production in the livestock industries. Over years several attempts, involving several different parameters of sperm cells, have been made to develop an efficient sperm sorting procedure. Till date, the only commercially available method for bovine sperm is flow cytometry. The disadvantages associated with this approach, however, points towards the need for developing a more non-invasive and cost efficient approach for separation of X and Y chromosome bearing spermatozoa. In this review paper, we attempt to highlight all the previously developed approaches, as well as other emerging immunological and proteomics based methods that might have the potential of being developed as a promising breakthrough approach for semen sexing. Furthermore, we discuss the advantages and applications of semen sexing in India and around the world.

Author Biography

AK Mohanty, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India

A K Mohanty, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Corresponding author email id: ashokmohanty1@gmail.com
$ Present address: Joint Director, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand

Himani Joshi#, Manya Mathur#
# Contributed equally

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