GERMINATION AND SEEDLING SURVIVALITY OF RICE (Oryza sativa  L.) UNDER ANOXIA

Deepjyoti Paul; Ranjan Das; Sangita Das; Hemendra Choudhury

doi:10.48165/

Authors

Deepjyoti Paul Department of Crop Physiology, Assam Agricultural University, Jorhat – 785 013, Assam (India)
Ranjan Das Department of Crop Physiology, Assam Agricultural University, Jorhat – 785 013, Assam (India)
Sangita Das Department of Crop Physiology, Assam Agricultural University, Jorhat – 785 013, Assam (India)
Hemendra Choudhury Department of Crop Physiology, Assam Agricultural University, Jorhat – 785 013, Assam (India)

DOI:

https://doi.org/10.48165/

Keywords:

Anoxia, amylase activities, coleoptiles, genotypes, germination, rice, stress tolerance

Abstract

A study was conducted at the Stress Physiology Laboratory, AAU, Jorhat, Assam (India) during kharif season 2016-2017. Rice genotypes (146 nos.) collected from different ecotypes were screened for anaerobic tolerance based on their survivality. Of the genotypes evaluated, six genotypes viz., ‘Ronga Lahi’, ‘Ronga Bao’, ‘Bangali Lahi’, ‘Suwag Moni Horu’, ‘Asra 123’ and ‘Asra 8’ were selected as anoxia tolerant genotypes on the basis of seed germination, seedling survival and growth. These genotypes were also evaluated for amylase activity. The result revealed a significant variation in genotypes during anoxic germination in terms amylase activity. Higher α- and β-amylase activities were observed in ‘Asra 123’, followed by ‘Asra 8’ as compared to the susceptible genotype ‘IR-64’. Increased amylases activity in tolerant genotypes might be attributed to the production and maintenance of sufficient amount of soluble sugar under anoxic condition during germination. The study revealed that ‘Asra 123’, ‘Asra 8’, ‘Ronga Lahi’, ‘Ronga Bao’, ‘Bangali Lahi’ and ‘Suwag Moni Horu’ could successfully be germinated and established under anoxic condition.

Downloads

Download data is not yet available.

References

Akazawa, T. and Hara-Mishimura, I. 1985. Topographic aspects of biosynthesis, extracellular section and intracellular storage of proteins in plant cells. Annual Review of Plant Physiology, 70: 441-472.

Angaji, S.A., Septiningsih, E.M., Mackill, D.J. and Ismail, A.M. 2010. QTLs associated with tolerance of flooding during germination in rice (Oryza sativa L.). Euphytica, 172(2): 159-168. Beck, E, and Ziegler, P. 1989. Biosynthesis and degradation of starch in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology, 40: 95-117.

Bhattacharya, A. and Vijaylaxmi. 2016. Carbohydrates. pp. 167-220. In: Biochemical Aspects of Plant Physiology., Mew India Publishing Agency, New Delhi, India.

Germination and seedling survivality of rice under anoxia 101

Damaris, R. N., Lin, Z., Yang, P. and He, D. 2019. The rice alpha-amylase, conserved regulator of seed maturation and germination. International Journal of Molecular Science, 20(2): 450. [doi: 10.3390/ijms20020450].

Fincher, G.B. 1989. Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annual Review of Plant Physiology and Plant Molecular Biology, 40: 305-345.

Gubler, F., Kalla, R., Roberts, J.K. and Jacobsen, J.V. 1995. Gibberellin-regulated expression of a Myb gene in barley aleurone cells: Evidence for Myb transactivation of a high-pI alpha amylase gene promoter. Plant Cell, 7: 1879-1891.

Guglielminetti, L., Yamaguchi, J., Perata, P. and Alpi, A. 1995. Amylolytic activities in cereal seeds under aerobic and anaerobic conditions. Plant Physiology, 109(3): 251-260. Livsley, M.A. and Bray, C.M. 1991. The effect of ageing upon α-amylase production and protein synthesis by wheat aleurone layers. Annals of Botany, 68(1): 69-73.

Magneschi, L. and Perata, P. 2009. Rice germination and seedling growth in the absence of oxygen. Annals of Botany, 103: 181-196.

Nandi, S., Das, G. and Sen-Mandi, S. 1995. β-amylase activity as an index for germination potential in rice. Annals of Botany, 75(5): 463-467.

Narsai, R., Edwards, J.M., Roberts, T.H., Whelan, J., Joss, G.H. and Atwell, B.J. 2015. Mechanisms of growth and patterns of gene expression in oxygen deprived rice coleoptiles. Plant Journal, 8: 25-40.

Panse, V.G. and Sukhatme, P.V. 1985. Statistical Methods for Agricultural Workers. Indian Council of Agricultural Research, New Delhi, India.

Perata, P., Pozueta-Romero, J., Akazawa, T. and Yamaguchi, J. 1992. Effect of anoxia on starch breakdown in rice and wheat seeds. Planta, 188(4): 611-618.

Septiningsih, E.M., Ignacio, J.C.I., Sendon, P.M.D., Sanchez, D.L., Ismail, A.M. and Mackill, D.J. 2013. QTL mapping and confirmation for tolerance of anaerobic conditions during germination derived from the rice landrace Ma-Zhan Red. Theoretical Applied Genetics, 126: 1357-1366.

Setter, T.L., Ellis, M., Laureles, C.V., Ella, E.S., Senadhira, D. and Mishra, S.B. 1997. Physiology and genetics of submergence tolerance in rice. Annals of Botany, 79: 67-77.

Tuong, T.P., Pablico, P.P., Yamauchi, M., Confesor, R. and Moody, K. 2000. Increasing water productivity and weed suppression of wet-seeded rice: effect of water management and rice genotypes. Experimental Agriculture, 36: 71-89.

Umarani, E., Hemalatha V., Bhadana, V.P., Senguttuvel, P., Subbarao, L.V., Neeraja, C.N., Yugandhar, R.P. and Kota, S. 2018. Screening of rice (Oryza sativa L.) genotypes for anaerobic germination. International Journal of Pure and Applied Bioscience, 6(5): 1318- 1325.

Yamauchi, M., Herradura, P.S. and Aguilar, A.M. 1994. Genotype difference in rice post germination growth under hypoxia. Plant Science, 100: 105-113.