ONE-STEP PREPARATION OF PROTEIN A/G-CONJUGATED CdSe/MSA  QUANTUM DOTS FOR ULTRAFAST WESTERN BLOT VISUALIZATION

Hong-Diep Thi Tran; Le Khanh-Thien; Hieu Tran-Van

doi:10.48165/

Authors

Hong-Diep Thi Tran Department of Molecular and Environmental Biotechnology, University of Science, Hochiminh City, Vietnam
Le Khanh-Thien Department of Molecular and Environmental Biotechnology, University of Science, Hochiminh City, Vietnam
Hieu Tran-Van Department of Molecular and Environmental Biotechnology, University of Science, Hochiminh City, Vietnam

DOI:

https://doi.org/10.48165/

Keywords:

CdSe/MSA, protein A/G (pA/G), Quantum Dots (QDs), Western blot

Abstract

Protein A/G (pA/G) has high affinity with the fragment crystallizable (Fc) region of antibodies so is used as a linker to attach antibodies to quantum dots (QDs), thereby helps the antigen-binding fragment (Fab) to expose outwardly. The present study deduced the interactive nature between pA/G and mercaptosuccinic acid-coated CdSe (CdSe/MSA) QDs by using different reagents to interfere the binding formation. Besides, the use of pA/G-conjugated QDs (QDs-pA/G) in Western blot was tested by using QDs-pA/G associated with anti-GST antibodies to label mPrPc-GST protein in Escherichia coli lysates. The results showed that most of the pA/G could not bind to QDs in presence of 2% sodium dodecyl sulfate, 0.1 ol and 1% tween 20. The study revealed that pA/G was mostly bound to CdSe/MSA QDs by non-covalent interactions. The QDs pA/G wassuccessfully applied in direct Western blot with a stronger signal

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