SURFACE STERILIZATION AND CALLUS INDUCTION POTENTIAL OF  VARIOUS INDICA RICE (Oryza sativa L.) VARIETIES

G B  Sawant; S V Sawardekar; M Y Patil; S M Jadhav

doi:10.48165/

Authors

G B Sawant Department of Agricultural Botany, College of Agriculture,, Dapoli, Ratnagiri – 415 712, Maharashtra (India)
S V Sawardekar Plant Biotechnology Centre, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Ratnagiri – 415 712, Maharashtra (India)
M Y Patil Department of Agricultural Botany, College of Agriculture,Dapoli, Ratnagiri – 415 712, Maharashtra (India)
S M Jadhav Department of Agricultural Botany, College of Agriculture,Dapoli, Ratnagiri – 415 712, Maharashtra (India)

DOI:

https://doi.org/10.48165/

Keywords:

2,4-D, callus induction protocol, proliferation potential, rice, surface sterilization

Abstract

The present study was conducted to standardize the efficient and reliable surface sterilization and callus induction protocol for 22 rice (Oryza sativa L.) varieties developed by Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Maharashtra (India) using different combinations of various plant growth regulators. The dehusked mature rice seed was used as explant. Prior to callus induction, surface sterilization protocol was standardized using 70% ethanol and different concentrations of mercuric chloride (HgCl2). The results revealed that surface sterilization treatment with 70% ethanol for 1 min, followed by 1.0% HgCl2 for 10 min and then washing the seeds 4-5 times was most effective in establishing maximum aseptic cultures (86.67%). Callus induction potential differed significantly among the rice varieties for the concentrations of plant growth regulators in MS medium. Rice cv. ‘Ratnagiri-711’ followed by cv. ‘Karjat-8’ were most superior in callus induction and proliferation potential with the respective callus induction frequency of 87.56 and 87.0%, and callus weight of 269.37 and 235.20 mg after 45 days of inoculation. The supplementation of 2,4-D @ 1.0 to 2.0 mg L-1 along with 0.5 mg L-1 BAP in MS medium was found optimum for embryogenic callus formation. All the calli formed on MS medium supplemented with combinations of plant growth regulators were yellowish-white in colour but with different textures as compact, soft, friable that were embryogenic and mucilaginous that were non embryogenic.

Downloads

Download data is not yet available.

References

Abe, T. and Futsuhara, Y. 1986. Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theoretical and Applied Genetics, 72: 3-10.

Ahmad, M., Nangyal, H., Imran, M. and Ullah, F. 2016. Optimization of protocol for surface sterilization and callus induction for three rice varieties. American-Eurasian Journal of Agricultural and Environmental Sciences, 16(2): 357-361.

Alam, M.F. 1994. Protoplast Culture and Transformation in Rice (Oryza sativa L.). Ph.D. (Genetics) Thesis. Faculty of the Graduate School, University of the Philippines, Los Banos, Philippines. Amrita Mukherjee, Islam Mohd., R., Nasiruddin, K.M. and Banerjee, Purba 2015. Callus initiation and plantlet regeneration ability of some rice genotypes. International Journal of Scientific and Technology Research, 4(10): 354-361.

Anita, Roy, Aich, S.S. and Mukherjee, S. 2012. Differential responses to indirect organogenesis in rice cultivars. International Journal of Scientific and Research Publications, 2(9): 1-5. Anonymous. 2016. FAOSTAT. [http://www.fao.org/faostat/en/#data/QC/visualize]. Carsono, N. and Yoshida, T. 2006. Identification of callus induction potential of 15 Indonesian rice genotypes. Plant Production Science, 9(1): 65-70.

Chen, T.H., Lam, L. and Chen, S.C. 1985. Somatic embryogenesis and plant regeneration from cultured young inflorescences of Oryza sativa L. (rice). Plant Cell Tissue and Organ Culture, 4: 41-54.

Datta, K., Datta, I. and Potrykus. 1990. Embryogenesis and plant regeneration from microspores of both indica and japonica rice. Plant Cell Reports, 9: 253-356.

DeFossard, R.A. 1986. Principles of plant tissue culture. pp. 1-13. In: Tissue Culture as a Plant Production System for Horticultural Crops. (ed. Zimmerman R.H., Griesbach R.J., Hammerschlag F.A., Lawson R.H.), Martinus Nijhoff Publ., Dordrecht, Netherlands.

Fujiwara, A. and Yatazama, M. 1964. Tissue culture in rice improvement: Status and potential. Advances in Agronomy, 41: 339-297.

Gomez, K.A. and Gomez, A.A. 1984. Statistical Procedure for Agricultural Research. (2nd edn.). John Wiley and Sons, New York, USA.

Gowda, H.R., Krishna, V., Kumara Swamy, G.K., Ramachandra, R. and Gowda, T.K.S. 2009. Standardization of regeneration protocol for indica rice varieties Rasi and IR-20. Mysore Journal of Agricultural Sciences, 43(3): 412-419.

Kataky, A. and Handique, P.J. 2010. Standardization of sterilization techniques prior to in vitro propagation of Andrographis paniculata. Asian Journal of Science and Technology, 6: 119- 122.

Libin, A., King, P.J.H., Ong, K.H., Chubo, J.K. and Sipen, P. 2012. Callus induction and plant regeneration of Sarawak rice (Oryza sativa L.) variety Biris. African Journal of Agricultural Research, 7(30): 4260-4265.

Mannan, M.A., Sarker, T.C., Akhter, M.T., Kabir, A.H. and Alam, M.F. 2013. Indirect plant regeneration in aromatic rice (Oryza sativa L.) var. ‘Kalijira’ and ‘Chinigura’. Acta Agriculturae Slovenica, 101(2): 231-238.

G.B. Sawant et al.

Morrish, F., Vasil, V. and Vasil, I.K. 1987. Developmental morphogenesis and genetic manipulation in tissue and cell cultures of the gramineae. Advances in Genetics, 24: 432-499. Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bioassay of tobacco tissue culture. Physiologia Plantarum, 15: 473-497.

Nishi, T., Yamada, Y. and Takahashi, E. 1968. Organ differentiation and plant regeneration in rice callus. Nature, 219: 508-509.

Oyebanji, O.B., Nweke, O., Odebunmi, O., Galadima, N.B., Idris, M.S., Nnodi, U.N., Afolabi, A.S. and Ogbadu, G.H. 2009. Simple, effective and economical explant-surface sterilization protocol for cowpea, rice and sorghum seeds. African Journal of Biotechnology, 8: 5395-5399.

Panjaitan, S.B., Abdullah, S.N.A., Aziz, M.A., Meon, S. and Omar, O. 2009. Somatic embryogenesis from scutellar embryo of Oryza sativa L. var. MR219. Pertanika Journal of Tropical Agricultural Science, 32: 185-194.

Poeaim, A., Poeaim, S., Poraha, R., Pongjaroenkit, S. and Pongthongkam, P. 2016. Optimization for callus induction and plant regeneration from mature seeds of Thai rice variety: Nam Roo (Oryza sativa L.) Bioengineering and Bioscience, 4(5): 95-99.

Poraha, R., Poeaim, A., Pongjaroenkit, S. and Pongthongkam, P. 2016. Callus induction and plant regeneration on optimization of the culture conditions in Jow Haw rice (Oryza sativa L.). International Journal of Agricultural Technology, 12(2): 241-248.

Raghawa Ram, N.V. and Nabors, M.W. 1984. Cytokinin mediated long term, high frequency plant regeneration in rice tissue cultures. Zeitschriftfur Pflanzenphysiologie, 113: 315-323. Ramesh, M., Murugiah, V. and Gupta, A.K. 2009. Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.). Indian Journal of Experimental Biology, 47: 68-74. Revathi, S. and Pillai, M.A. 2011. In vitro callus induction in rice (Oryza sativa L.) Research in Plant Biology, 1(5): 13-15.

Sah, S.K., Ajinder Kaur and Sandhu, J.S. 2014. High frequency embryogenic callus induction and whole plant regeneration in Japonica rice cv. Kitaake. Journal of Rice Research, 2(2): 1-5. Sananda Mondal, Singh, R.P. and Bose, Bandana. 2013. Standardization of culture medium for somatic embryogenesis of rice var. MTU 7029. International Journal of Bio-resource and Stress Management, 4(4): 500-505.

Sankepally, S.S.R. and Singh, B. 2016. Optimization of regeneration using differential growth regulators in indica rice cultivars. Biotech (Protocols and Methods), 6: 1-19. Shukla, R., Dube, A. and Koshy, E.P. 2014. Production of high quality embryogenic callus of rice. Bioscan, 9(3): 1077-1080.

Sripichitt, P. and Cheewasestathum, P. 1994. Plant regeneration from embryo derived callus of aromatic rice (Oryza sativa L.) variety Khao Dawk Mali 105. Kasetsart Journal (Natural Science), 28: 27-37.

Ullah, H., Ullah, I., Jadoon, S.A. and Rashid, H. 2007. Tissue culture techniques for callus induction in rice. Sarhad Journal of Agriculture, 23(1): 81-86.

Verma, D., Joshi, R., Shukla, A. and Kumar, P. 2011. Protocol for in vitro somatic embryogenesis and regeneration of rice (Oryza sativa L.). Indian Journal of Experimental Biology, 49: 958- 963.

Visarada, K.B.R.S., Sailaja, M. and Sarma, N.P. 2002. Effect of callus induction media on morphology of embryogenic calli in rice genotypes. Biologia Plantarum, 45: 495-502.