FACILE SYNTHESIS AND SIZE DISTRIBUTION ANALYSIS OF SILVER  NANOPARTICLES OF FUNGUS Penicillium crustosum, ISOLATED FROM  Taxus baccata Linn.

Ankush Sharma; Anand Sagar

doi:10.48165/

Authors

Ankush Sharma Department of Biosciences, Himachal Pradesh University, Summerhill, Shimla -171 005, Himachal Pradesh (India)
Anand Sagar Department of Biosciences, Himachal Pradesh University, Summerhill, Shimla -171 005, Himachal Pradesh (India)

DOI:

https://doi.org/10.48165/

Keywords:

Energy dispersive spectroscopy, HRTEM, Penicillium crustosum, silver nanoparticles, Taxus baccata

Abstract

The present study was aimed at the synthesis and size distribution analysis of silver nanoparticles (AgNPs), from a fungal endophyte Penicillium crustosum. The fungus was isolated from the needles of Taxus baccata Linn. and identified as Penicillium crustosum by molecular characterization. The fungal extract of P. crustosum was subjected to the synthesis of AgNPs. UV vis-spectroscopy confirmed the formation of AgNPs by forming the specific peaks at 390-450 nm. The lyophilized AgNPs were subjected to size distribution analysis using tools like X-ray diffraction, energy dispersive spectroscopy (EDS), field emission scanning electron microscopy (FEG SEM), and high-resolution transmission electron microscopy (HRTEM). The average crystalline size of AgNPs was calculated by Debye-Scherrer’s formula which showed AgNPs of 36.93 nm. These findings substantiated the results of FEG-SEM and HRTEM, where the average size was below 50 nm. Dynamic light scattering results showed Z-average of AgNPs as 352.0 rnm with 0.982 PdI. Zeta potential was -19.1 mV which showed the capacity of formed AgNPs to decline agglomeration. EDS analysis proved that the requisite phase of silver is present in the sample. The present study displayed the potential of P. crustosum as reducing agent which converted silver ions into AgNPs in eco-friendly and cost-effective manner.

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