In vitro EVALUATION OF ANTIDIABETIC, ANTIOXIDANT AND  PROBIOTIC ATTRIBUTES OF Lactobacillus casei strain NCDC 357

Verma Mukesh; Saharan Baljeet Singh

doi:10.48165/

Authors

Verma Mukesh Department of Microbiology, Kurukshetra University, Kurukshetra - 136 119, Haryana (India)
Saharan Baljeet Singh Department of Microbiology, CCS Haryana Agricultural University, Hisar - 125 004, Haryana (India)

DOI:

https://doi.org/10.48165/

Keywords:

Antidiabetic activity, antioxidant activity, Lactobacillus casei, probiotic, strain evaluation

Abstract

The present study was aimed to evaluate the antidiabetic and antioxidant activities of a lactic acid bacterial strain ‘Lactobacillus casei NCDC 357’, and to investigate its probiotic characteristics. The antidiabetic activity was examined by evaluating the α-glucosidase and α-amylase inhibitory activities. L. casei strain NCDC 357 exhibited antidiabetic activities by α glucosidase (67.7% and IC50 38.08 μL) and α-amylase (53.6% and IC50 49.96 μL). The strain showed antioxidant activities by DPPH (53.4% and IC50 77.60 μL) and ABTS (48.7% and IC50 51.48 μL). Further, the strain was resistant to simulated gastrointestinal juice and showed potential for health promotiobased on adhesion, antibiotic susceptibility, and anti microbial activities. L. casei NCDC 357 appeared to be a potential probiotic having beneficial health effects.

Downloads

Download data is not yet available.

References

Ashwin, T., Deswal, S. and Saharan, B.S. 2018. Production and characterization of biosurfactants from various Lactobacillus species: A bioremediation technique. pp. 109-123. In: International Conference on Sustainable Technologies for Intelligent Water Management. 16- 19 February, 2018. Indian Water Resources Society (IWRS)/ Department of Water Resources Development & Management, IIT Roorkee, India.

Barzegar, H., Behbahani, B.A. and Falah, F. 2021. Safety, probiotic properties, antimicrobial activity, and technological performance of Lactobacillus strains isolated from Iranian raw milk cheeses. Food Science and Nutrition, 9(8): 4094-4007.

Bindu, A. and Lakshmidevi, N. 2021. Identification and in vitro evaluation of probiotic attributes of lactic acid bacteria isolated from fermented food sources. Archieves of Microbiology, 203: 579-595.

Charteris, W.P., Kelly, P.M., Morelli, L. and Collins, J.K. 1998. Antibiotic susceptibility of potentially probiotic Lactobacillus species. Journal of Food Protection, 61: 1636-1643. Chaudhary, A. and Saharan, B.S. 2019. Probiotic properties of Lactobacillus plantarum. Journal of Pure and Applied Microbiology, 13(2): 933-948.

Chaudhary, A. and Saharan, B.S. 2018. Acid and bile tolerance of Lactobacillus plantarum isolated from dosa batter. pp. 78-89. In: International Conference on Microbes for Biotechnology Innovations (MBI- 2018). 7 December 2018. Dr. Radha Krishnan Foundation Fund & Association of Microbiologist of India, Department of Microbiology, Maharshi Dayanand University Rohtak, India.

Chen, P., Zhang, Q., Dang, H., Liu, X., Tian, F., Zhao, J., Chen, Y., Zhang, H. and Chen, W. 2014. Screening for potential new probiotic based on probiotic properties and α-glucosidase inhibitory activity. Food Control, 35: 65-72.

Choi, K., Choi, S.I., Park, M.W. and Han, J.S. 2017. Cyanidin-3-O-glucoside ameliorates post prandial hyperglycemia in diabetic mice. Journal of Life Science, 27: 32-37. Cizeikiene, D. and Jagelaviciute, J. 2021. Investigation of antibacterial activity and probiotic properties of strains belonging to Lactobacillus and Bifidobacterium genera for their potential application in functional food and feed products. Probiotics Antimicrobial Proteins, 13: 1387- 1403.

de Souza, B.M.S., Borgonovi, T.F., Casarotti, S.N., Todorov, S.D. and Penna, A.L.B. 2019. Lactobacillus casei and Lactobacillus fermentum strains isolated from Mozzarella cheese: Probiotic potential, safety, acidifying kinetic parameters and viability under gastrointestinal tract conditions. Probiotics Antimicrobial Protein, 11(2): 382-396.

Dileep, K.V., Nithiyanandan, K. and Remya, C. 2018. Binding of acarbose, an anti-diabetic drug to lysozyme: A combined structural and thermodynamic study. Journal of Biomolecular Structure and Dynamics, 36: 3354-3361.

FAO/WHO. 2002. Guidelines for the Evaluation Of Probiotics in Food. FAO, Paris, France. [https://scholar.google.com/scholar_lookup?journal=Guidelines+for+the+Evaluation+Of+P robiotics+in+Food%2E&publication_year=2002&pages=1%E2%80%9311]

García-Cayuela, T., Korany, A. and Martínez-Cuesta, M.C. 2014. Adhesion abilities of dairy Lactobacillus plantarum strains showing an aggregation phenotype. Food Research International, 57: 44-50.

Verma Mukesh and Saharan Baljeet Singh

Gazza, L., Gazzelloni, G., Taddei, F., Latini, A., Muccilli, V., Alfieri, M., Conti, S., Redaelli, R. and Pogna, N.E. 2016. The starch-bound alpha-amylase/trypsin-inhibitors in Avena. Molecular Genetics Genomics Medicine, 291: 2043-2054.

Gerard, C. and Vidal, H. 2019. Impact of gut microbiota on host glycemic control. Frontiers in Endocrinology, 10: 29-34.

Gurung, M., Li, Z., You, H., Rodrigues, R., Jump, D.B., Morgun, A. and Shulzhenko, N. 2020. Role of gut microbiota in type 2 diabetes pathophysiology. EBioMedicine, 51: 102590 [10.1016/j.ebiom.2019.11.051].

Herrema, H. and Niess, J.H. 2020. Intestinal microbial metabolites in humna metabolism and type 2 diabetes. Dieteologia, 63: 2532-2547.

Kao, T.H. and Chen, B.H. 2006. Functional components in soybean cake and their effects on antioxidant activity. Journal of Agricultral and Food Chemistry, 54: 7544-7555. Kaushik, J.K., Kumar, A. and Duary, R.K. 2009. Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum. Plos One, 4: 1-11.

Kiani, A., Nam, Y., Hedayati, S., Komi, D.A.E., Goudarzi, F. and Haghshenas, B. 2021a. Application of Tarkhineh fermented product to produce potato chips with strong probiotic properties, high shelf-life, and desirable sensory characteristics. Frontiers in Microbiology, 12: 657579.[doi.org/10.3389/fmicb.2021.657579].

Kiani, A., Nami, Y., Hedayati, S., Jaymand, M., Samadian, H. and Haghshenas, B. 2021b. Tarkhineh as a new microencapsulation matrix improves the quality and sensory characteristics of probiotic Lactococcuslactis KUMS-T18 enriched potato chips. Scientific. Reports, 11(1): 1-13.

Krausova, G., Hyrslova, I. and Hynstova, I. 2019. In vitro evaluation of adhesion capacity, hydrophobicity, and auto-aggregation of newly isolated potential probiotic strains. Fermentation, 5(4): 100-107.

Kumar, S., Narwal, S., Kumar, V. and Prakash, O. 2011. α-glucosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacognosy Reviews, 5: 19. [doi: 10.4103/0973- 7847.79096].

Kumar, V., Khan, A.A., Tripathi, A., Dixit, P.K. and Bajaj, U.K. 2015. Role of oxidative stress in various diseases: relevance of dietary antioxidants. Journal of Phytopharmacology, 4: 126-132 Li, S., Zhao, Y., Zhang, L., Zhang, X., Huang, L., Li, D., Niu, C., Yang, Z. and Wang, Q. 2012. Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods. Food Chemistry, 135: 1914-1919.

Lin, M.Z., Chai, W.M., Zheng, Y.L., Huang, Q. and Ou-Yang, C. 2019. Inhibitory kinetics and mechanism of rifampicin on α-glucosidase: Insights from spectroscopic and molecular docking analyses. International Journal of Biological Macromolecules, 122: 1244-1252.

Mannan, S.J., Rezwan, R., Rahman, S. and Begum, K. 2017. Isolation and Biochemical Characterization of Lactobacillus species from Yogurt and Cheese samples in Dhaka metropolitan area. Bangladesh Pharmaceutical Journal, 20(1): 27-33.

Marella, S., Hema, K., Shameer, S. and Prasad, T. 2020. Nano-ellagic acid: Inhibitory actions on aldose reductase and α-glucosidase in secondary complications of diabetes, strengthened by in silico docking studies. 3 Biotech., 10: 439. [doi: 10.1007/s13205-020-02411-1].

Maryam, A.S. and Bakr, A. 2018. Antimicrobial activities of lactic acid bacteria strains isolated from human breast milk against human pathogenic strains. International Journal of Clinical and Developental Anatomy, 4(1): 27-31.

Morrison, D.J. and Preston, T. 2016. Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism. Gut Microbes, 7: 189-200.

Phaniendra, A., Jestadi, D.B. and Periyasamy, L. 2015. Free radicals: Properties, sources, targets, and their implication in various diseases. Indian Journal of Clinical Biochemistry, 30(1): 11-26. [doi: 10.1007/s12291-014-0446-0].

Punthakee, Z., Goldenberg, R. and Katz, P. 2018. Definition, classification and diagnosis of diabetes, prediabetes and metabolic syndrome. Canadian Journal of. Diabetes, 42: 10-15.

Probiotic attributes of Lactobacillus casei 297

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26: 1231-1237.

Rowland, I., Gibson, G., Heinken, A., Scott, K., Swann, J., Thiele, I. and Tuohy, K. 2018. Gutmicrobiota functions: Metabolism of nutrientsand other food components. Europian Journal of Nutrition, 57(1): 1-24.

Saadatzadeh, A., Fazeli, R.M., Jamalifar, H. and Dinarvand, R. 2013. Probiotic properties of lyophilized cell free extract of Lactobacillus casei. Jundishapur Journal of Natural Pharmaceutical Products, 8(3): 131-137.

Saharan, B.S., Dilbaghi, N. and Sharma, S. 1998. Optimization of medium conditions for the production of bacteriocin-SN21 by Lactobacillus acidophilus. Indian Jornal of Microbiology, 38: 225-227.

Sharma, C., Gulati, S., Thakur, N., Singh, B.P., Gupta, S., Kaur, S., Mishra, S.K., Puniya, A.K., Gill, J.P. and Pahwa, H. 2017. Antibiotic sensitivity pattern of indigenous lactobacilli isolated from curd and human milk samples. 3 Biotech., 7(1): 53. [doi: 10.1007/s13205-017-0682-0].

Sharma, D. and Saharan, B.S. 2014. Simultaneous production of biosurfactants and bacteriocins by probiotic Lactobacilluscasei MRTL3. International Journal of Microbiology, 2014: 1-7. [doi.org/10.1155/2014/698713].

Sharma, D. and Saharan, B.S. 2016. Functional characterization of biomedical potential of biosurfactants produced by Lactobacillus helveticus. Biotechnology Reports, 11: 27-35. Sharma, D., Saharan, B.S., Chauhan, N., Bansal, A. and Procha, S. 2014. Production and structural characterization of Lactobacillus helveticus derived biosurfactant. The Scientific World Journal, Volume 2014, 493548. [http://dx.doi.org/10.1155/2014/493548].

Tabrizi, R., Moosazadeh, M., Lankarani, K.B., Akbari, M., Heydari, S.T., Kolahdooz, F. and Asemi, Z. 2018. The effects of synbiotic supplementation on glucose metabolism and lipid profiles in patients with diabetes: A systematic review and meta-analysis of randomized controlled trials. Probiotics and Antimicrobial Proteins, 10: 329-342.

Vankudre, M., Balpande, A. and Athale, M. 2015. Comparative analysis of α-amylase inhibition and antioxidant activity of whey from cow and buffalo milk fermented with lactobacillus species. Bioscience Biotechnology Research Communication, 8: 25-28.

Verma, M. and Saharan, B.S. 2020. In vitro assessment of the Probiotic properties and Bacteriocinogenic potential of Lactic acid bacteria from buffalo milk and curd in Haryana. Advances in Bioresearch, 11(5): 115-122

Wang, H. and Li, L. 2022. Comprehensive evaluation of probiotic property, hypoglycemic ability and antioxidant activity of lactic acid bacteria. Foods, 11, 1363. [doi: 10.3390/foods11091363]. Wang, Y., Wu, Y., Wang, Y., Xu, H., Mei, X., Yu, D., Wang, Y. and Li, W. 2017. Antioxidant properties of probiotic bacteria. Nutrients, 9: 521. [doi: 10.3390/nu9050521]. Wegh, C.A., Geerlings, S.Y., Knol, J., Roeselers, G. and Belzer, C. 2019. Postbiotics and their potential applications in early life nutrition and beyond. International Journal of Molecular Science, 20: 46-73.

Won, G.Y., Choi, S.I., Park, N.Y., Kim, J.E., Kang, C.H. and Kim, G.H. 2021. In vitro antidiabetic, antioxidant activity, and probiotic activities of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei strains. Current Microbiology, 78: 3181-3191.

Zarate, G., Chaia, A.P. and González, S. 2006. Viability and β-galactosidase activity of dairy propionibacteria subjected to digestion by artificial gastric and intestinal fluids. Journal of Food Protection, 63: 1214-1221.

Zeng, Z., Luo, J., Zuo, F., Zhang, Y. and Ma, H. 2016. Screening for potential novel probiotic Lactobacillus strains based on high dipeptidyl peptidase IV and α-glucosidase inhibitory. Journal of Functional Foods, 20: 486-495.