CHROMATOGRAPHIC ANALYSIS OF Blumea lanceolaria (Roxb.) Druce LEAF  EXTRACT: A TRADITIONAL HERBAL DRUG OF NORTH-EAST INDIA

Seema Khakhalary; Silistina Narzari; Jatin Sarmah; Monish Paul; Tapan Dutta

doi:10.48165/

Authors

Seema Khakhalary Department of Biotechnology, Bodoland University, Kokrajhar - 783 370, Assam (India)
Silistina Narzari Department of Biotechnology, Bodoland University, Kokrajhar - 783 370, Assam (India)
Jatin Sarmah Department of Biotechnology, Bodoland University, Kokrajhar - 783 370, Assam (India)
Monish Paul Department of Botany, Ganesh Lal Choudhury College, Barpeta Rd, Barpeta - 781 315, Assam (India)
Tapan Dutta Department of Botany, Jawaharlal Nehru College, Boko, Kamrup - 781 123, Assam (India)

DOI:

https://doi.org/10.48165/

Keywords:

Bioactive, Blumea lanceolaria extracts, medicinal herb, phytochemicals, solvents

Abstract

The North-Eastern region of India is hotspot of many medicinal herbs. Blumea lanceolaria from family Asteraceae is a traditional medicinal herb of North East India. The present study was aimed to screen the phyto chemical constituents and identify bioactive compounds in crude extracts of B. lanceolaria using gas chromatography-mass spectroscopy. The extracts were obtained by Soxhlet extraction in three solvents (methanol, n-hexane, and chloroform). The identification and characterization of bioactive compounds in the extracts was performed using GC-MS. Qualitative phytochemical screening was done for the presence of flavonoids, phenols, phytosterols, tannins and alkaloids. The study showed that thphytochemicals were higher in methanolic extract than exane-extracts. In GC-MS analysis a total of five compounds were detected in each sample. Chloroacetic acid, tetradecyl ester was found in methanol (18.15%) as well as in n-hexane extracts (18.83%) in diverse quantities. Likewise,compounds including 3, 7, 11, 15- tetramethyl-2-hexadecen-1-ol and Z,z-6,28-heptatriactontadien-2-one were and chloroform extracts. 1-Heptacosanol was identified in both n-hexane and chloroform extracts. The study reveals that crude extracts of B. lanceolaria is a potential source of naturally occurring s, so may be used in traditional medicines.

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References

Ahmed, F.A., Rahman, A. and Mubassara, S. 2014. Phytochemical composition, antioxidant activity and cytotoxicity of Blumea lacere Linn. from two different habitats. Journal of Biological Science, 3: 37-45.

Al-Abd, N.M., Mohamed, Z.N., Mansor, M., Azhar, F., Hasan, M.S. and Kassim, M. 2015. Antioxidant, antibacterial activity, and phytochemical characterization of Melaleu cacajuputi extract. BMC Complementary Alternative Medicine, 15: 1-13.

Alebiosu, C.O. and Yusuf, A.J. 2015. Phytochemical screening, thin-layer chromatographic studies and UV analysis of extracts of Citrullus lanatus. Journal of Pharmaceutical Chemical and Biological Science, 3: 214-220.

Awad, B. and Fink, C.S. 2000. Phytosterols as anticancer dietary components: Evidence and mechanism of action. Journal of Nutrition, 130: 2127-2130.

Belkacem, N., Djaziri, R., Lahfa, F., El-Haci, I.A. and Boucherit, Z. 2013. Phytochemical screening and in vitro antioxidant activity isolated bioactive compounds from Tridax procumbens Linn. Pakistan Journal of Biological Science, 16: 1971-1977.

Berdeaux, O., Voinot, L., Angioni, E., Juanéda, P. and Sebedio, J.L. 1998. A simple method of preparation of methyl trans-10, cis-12- and cis-9, trans-11-octadecadienoates from methyl linoleate. Journal of the American Oil Chemists Society, 75: 1749-1755.

Chawngkunga, C. 2005. Medicinal plants used as healing agents by the ethnic people of Mizoram. Lois Bet, Chandmari, Aizawl, Mizoram, India

Chung, K., Wong, T., Wei, C., Huang, Y. and Lin, Y. 1998. Tannins and human health: A review. Critical Review in Food Science and Nutrition, 38: 421-464.

Cuong, N.X., Hanh, T.T.H., Hang, L.T.T., Giang, V.H., Trung, N.Q., Thanh, N.V., Quong, T.H. 2021. Chemical constituents of Blumea balsamifera. Phytochemistry Letters, 43: 35-39. Epand, R., Savage, P. and Epand, R. 2007. Bacterial lipid composition and the antimicrobial

efficacy of cationic steroid compounds (Ceragenins). Biochimicaet Biophysica Acta – Biomembranes, 1768: 2500-2509.

Evans, W.C. 2009. Trease and Evans Pharmacognosy. Saunders Limited, London, UK. Furlan, C.P.B., Marques, A.C., Marineli, R.S. and Marostica, M.R. 2013. Conjugated linoleic acid and phytosterols counteract obesity induced by high-fat diet. Food Research International, 51: 429-435.

Garaniya, N. and Bapodra, A. 2014. Ethnobotanical and phytopharmacological potential of Abrus precatorius L.: A review. Asian Pacific Journal of Tropical Biomedicine, 4: 27-34. Harborne, J.B. 1998. Phytochemical methods: A guide to modern techniques of plant analysis. Chapman & Hall, London, UK.

Hema, R., Kumaravel, S., Gomathi, S. and Sivasubramaniam, C. 2010. Gas chromatography-mass spectroscopic analysis of Lawsonia inermis leaves. New York Science Journal, 3: 141-143. Jha, R.R. and Verma, S.K. 1996. Ethnobotany of Sauria paharias of Santhal Pargana, Bihar, Indian medicinal plants. Ethnobotany, 8: 31-35.

Just, M.J., Giner, R.M., Recio, C.M., Cuellar, M.J., Manez, S., Bilia, A.R. and Rios, J.L. 1998. Anti-inflammatory activity of unusual lupane saponins from Bupleurum fruticesens. Planta Medica, 64: 404-407.

Krishnamoorthy, K. and Subramaniam, P. 2014. Phytochemical profiling of leaf, stem, and tuber parts of Solenaamplexi caulis (Lam.) Gandhi using GC-MS. International Scholarly Research Notice, 2014: 1-13. [https://doi.org/10.1155/2014/567409].

Li, M., Zhou, L., Yang, D., Li, T. and Li, W. 2012. Biochemical composition and antioxidant capacity of extracts from Podophyllum hexandrum rhizome. BMC Complementary Alternative Medicine, 12: 263-263.

Chromatographic analysis of Blumea lanceolaria extract 103

Lou, H., Han, X. and Shen, T. 2007. Dietary polyphenols and their biological significance. International Journal of Molecular Science, 8: 950-988.

Mahato, S.B. and Sen, S. 1997. Advances in triterpenoid research, 1990-1994. Phytochemistry, 44: 1185-1236.

Mallikadevi, T., Paulsamy, S., Jamuna, S. and Karthika, K. 2012. Analysis for phytoceuticals and bioinformatics approach for the evaluation of therapeutic properties of whole plant methanolic extract of Mukia maderaspatana (L.) M. Roem. (Cucurbitaceae) - A traditional medicinal plant in western districts of Tamil Nadu, India. Asian Journal of Pharmaceutical Clinical Research, 5: 163-168.

Massish, R.M.A., Othman, L. and Sleiman, A. 2019. Antimicrobial activity of polyphenols and alkaloids in middle eastern plants. Frontiers in Microbiology, 10: 1-28. [DOI: 10.3389/ fmicb.2019.00911].

Navarro, A., De, l.H.B. and Villar, A. 2001. Anti-inflammatory and immunomodulating properties of a sterol fraction from Sideritis foetens. Biological & Pharmaceutical Bulletin, 24: 470-473. Nostro, A., Germano, M.P., Danelo, V., Marino, A. and Cannatelli, M.A. 2000. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology, 30: 379-384.

Okwu, D.E. and Josiah, C. 2006. Evaluation of the chemical composition of two Nigerian medicinal plants. African Journal of Biotechnology, 5: 357-361.

Ouyang, H., Kong, X.Z, He, W., Qin, N., He, Q.N., Wang, Y., Wang, R. and Xu, F. 2012. Effects of five heavy metals at sub-lethal concentrations on the growth and photosynthesis of Chlorella vulgaris. Chinese Science Bulletin, 57: 3363-3370.

Pappachen, L.K., Jyothilekshmy, V., Anjali, N.G., Vyshak, K., Radhila, R.N., Amrita, M. and Jifitha, B. 2019. Phytochemical screening and antimicrobial activity of Blumea mollis (D. Don) Merrill. Asian Journal of Pharmaceutical and Clinical Research, 12: 2455-3891.

Qin, L., He, Y., Tan, D., Yang, Z., Zhang, Q., Ling, H., Du, Y., Lu, Y., Xie, T. and Zhou, X. 2020. Simultaneous quantitative determination of polyphenolic compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by high-performance liquid chromatography with photodiode array detector. International Journal of Analytical Chemistry, 2020, 1-9.

Rai, P.K. and Lalramnghinglova, H. 2010. Ethnomedicinal plant resources of Mizoram, India: Implication of traditional knowledge in healthcare system. Ethnobotanical Leaflets, 14: 274- 305.

Rajalakshmi, K. and Mohan, V.R. 2016. GC-MS analysis of bioactive components of Myxopyrum serratum A.W. Hill (Oleaceae). International Journal of Pharmaceutical Sciences Review and Research, 38: 30-35.

Razika, L., Thanina, A.C., Nadjiba, C.M., Narimen, B., Mahdi, D.M. and Karim, A. 2017. Antioxidant and wound healing potential of saponins extracted from the leaves of Algerian Urtica dioica L. Pakistan Journal of Pharmaceutical Science, 30: 1023-1029.

Reddy, D.N. 2019. Essential oils extracted from medicinal plants and their applications. Natural Bio-active Compounds, 2: 237-282.

Saikia, K., Lalawmpuii, R. and Borgohain, P. 2017. Evaluation of in vitro antioxidant and cytotoxic activity of methanolic leaf extract of Blumea lanceolaria Roxb. International Journal of Technical Research and Applications, 5: 54-57.

Satyal, P., Dosoky, N.S., Poudol, A. and Setzer, W.N. 2012. Essential oil constituents and their biological activities from the leaves of Cassia fistula growing in Nepal. Open Access Journal of Medical and Aromatic Plants, 3: 1-4.

Sawmliana, M. 2003. The Book of Mizoram Plants. Lois Bet, Chandmari, Aizawl, Mizoram, India. Shyam, P. and Suresh, P.K. 2013. Comparative analysis of three leaf extracts of Ixora coccinea Linn. for their protective and antioxidant potentials and correlation with analytical data. International Journal of Pharma and BioSciences, 4: 937-949.

Seema Khakhalary et al.

Spanova, M. and Drum, G. 2011. Squalene-biochemistry, molecular biology, press biotechnology and applications. European Journal of Lipid Science and Technology, 113: 1299-1320. Starlin, T., Prabha, P.S., Thayakumar, B.K.A. and Gopalakrishnan, V.K. 2019 Screening and GC MS profiling of ethanolic extract of Tylophora pauciflora. Bioinformation, 15: 425-429. Swaraz, A.M., Sultana, F., Bari, W.M., Ahmed, K.S., Hasan, M., Islam, M.M., Islam, M.A., Satter, M.A., Hossian, M.H., Islam, M.S., Khan, M.I. and Raihan, M.O. 2021. Phytochemical profiling of Blumea laciniata (Roxb.) and its phytopharmaceutical potential against diabetic, obesity, and Alzheimer’s. Biomedicine and Pharmacotherapy, 141: 111859. [https://doi.org/ 10.1016/j.biopha.2021.111859].

Terblanche, U., Semakalu, S.C.C., Mtunzi, F. and Pillay, M. 2017. Screening of variables influencing extraction yield of Cotyledonor biculata: 23 full factorial design. International Journal of Pharmacognosy and Phytochemical Research, 9: 303-312.

WHO. 2002. WHO Traditional Medicine Strategy 2002-2005. World Health Organization, Geneva. Switzerland. WHO/EDM/TRM/2002. TMS_en.pm (who.int).