Insilico STUDIES ON ANTIMICROBIAL PEPTIDES (AMPs) FROM Calliphora vicina, THE BLOW FLY OF FORENSIC IMPORTANCE

Shyamasree Ghosh; Rashmi Bhattacherjee; Dhriti Banerjee

doi:10.48165/

Authors

Shyamasree Ghosh School of Biological Sciences, National Institute of Science Education and Research (NISER), Bhubaneswar – 752 050, Odisha (India)
Rashmi Bhattacherjee Zoological Survey of India, Prani Vigyan Bhawan, M-Block, New Alipore, Kolkata - 700 053, West Bengal (India)
Dhriti Banerjee Zoological Survey of India, Prani Vigyan Bhawan, M-Block, New Alipore, Kolkata - 700 053, West Bengal (India)

DOI:

https://doi.org/10.48165/

Keywords:

Antimicrobial peptide, Calliphora vicina, cecropin

Abstract

The forensic flies colonize and feed on carcasses and cadavers and draw their nourishment from the dead and decomposed matter. They find significance in forensic studies as nutrient recyclers, disease vector and reveal association with pathogenic microorganisms. Despite their importance in forensistudies, their innate immune defense molecules like antimicrobial peptides (AMPs), have not been well characterized. Therefore, we characterized the AMPs from forensic fly, Calliphora vicina. using MSA, NCBI Tree Viewer, I-TASSER, Peptide cutter, NetPhos 3.1, Prot param Expasy insilico tools, for prediction of homology and evolutionary relationships, structure, function, potential cleavage, phosphorylation sites and physicochemical properties. We report for the first time thdifferences in the structures of C. vicina AMPs and the presence of nucleic acid-binding domain in Cecropin, at aspartic acid (D19), glutamine (Q15), histidine (H16) probably contributing to the antiviral immune responses. This in-silico study highlights the diversity of AMPs in forensic flies and reveals their indepth structural information. Further, these AMPs partially explain their association with pathogenic microorganisms.

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