BIOSORPTION OF HEAVY METALS FROM TANNERY EFFLUENTS BY USING GREEN UNICELLULAR MICROALGA, Tetradesmus obliquus RDRL01

Authors

  • A M Rajalakshmi Fermentation Technology Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Salem – 636 011, Tamil Nadu (India)
  • T Silambarasan School of Allied Health Sciences, Vinayaka Mission’s Research Foundation (Demeed to be University), Salem – 636 308, Tamil Nadu (India)
  • R Dhandapani Fermentation Technology Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Salem – 636 011, Tamil Nadu (India)

DOI:

https://doi.org/10.48165/

Keywords:

Bioethanol, biosorption, FTIR, heavy metals, HPLC, microalgae, tannery effluent

Abstract

In present study, ecofriendly biological treatment of tannery effluent was  performed by using unicellular microalga Tetradesmus obliquus strain  RDRL01, isolated from Sipcot, Vellore, Tamil Nadu (India). The microalga  reduced heavy metals content viz., chromium, cobalt, nickel, cadmium, lead,  zinc and copper by 99.1, 98.2, 97.4, 94.3, 98.3, 96.8 and 96.3%, respectively, after 15 days treatment of tannery effluent. Treated effluent had 3.51 g biomass L-1 and treated effluent biomass contained 330.5 mg carbohydrate  g-1 and yielded 13.5 mL bioethanol g-1. The functionagroups identified by  Fourier transform infrared analysis were carboxylic acids, alkyl, hydroxyl,  and carbonyl. Purified bioethanol was confirmed by HPLC, and chromatogram peak showed the retention time of 5.35 min. Microalgae T. obliquus RDRL01 demonstrated good biosorption efficiency and removed various  toxic heavy metal pollutants from tannery effluent. 

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References

Abou-Shanab, R.A., El-Dalatony, M.M., El-Sheekh, M.M., Ji, M.K., Salama, E.S., Kabra, A.N. and Jeon, B.H. 2014. Cultivation of a new microalga, Micractinium reisseri, in municipal wastewater for nutrient removal, biomass, lipid, and fatty acid production. Biotechnology and Bioprocess Engineering, 19(3): 510-518.

Andersen, R.A. 2005. Algal Culturing Techniques. Elsevier, Burlington, USA. APHA. 2005. Standard Methods for the Examination of Water and Wastewater. American Public Health Association. Washington, USA.

Aslam, S. and Yousafzai, A.M. 2017. Chromium toxicity in fish: A review article. Journal of Entomology and Zoology Studies, 5(3): 1483-1488.

Baskar, K.N.V. and Abdul Raheem, A. 2011. Impact of Tannery Industry on Livelihoods: A Study on Palar River Basin in India. LAP Lambert Academic Publishing, India, ISBN-13: 9783847325772, 108.

Chen, C.Y., Chang, H.Y. and Chang, J.S. 2016. Producing carbohydrate-rich microalgal biomass grown under mixotrophic conditions as feedstock for biohydrogen production. International Journal of Hydrogen Energy, 41(7): 4413-4420.

Das, C., Naseera, K. and Ram, A. 2017. Bioremediation of tannery wastewater by a salt-tolerant strain of Chlorella vulgaris. Journal of Applied Phycology, 29: 235-243.

Das, C., Ramaiah, N., Pereira, E. and Naseera, K. 2018. Efficient bioremediation of tannery wastewater by monostrains and consortium of marine Chlorella sp. and Phormidium sp. International Journal of phytoremediation, 20(3): 284-292.

Dwaish, A.S., Mohammed, D.Y., Jawad, A.M. and AL-Kubaicy, A.A. 2011. Determine the uptake of lead in Chlorella vulgaris isolated from Tigris river in Baghdad city. International Journal of Scientific and Engineering Research, 2(9): 1-4.

Feng, P., Deng, Z., Hu, Z. and Fan, L. 2011. Lipid accumulation and growth of Chlorella zofingiensis in flat plate photobioreactors outdoors. Bioresource Technology, 102(22): 10577- 10584.

Fontoura, J.T., Rolim, G.S., Farenzena, M. and Gutterres, M. 2017. Influence of light intensity and tannery wastewater concentration on biomass production and nutrient removal by microalgae Scenedesmus sp. Process Safety and Environmental Protection, 111: 355-362.

Gimpel, J.A., Henríquez, V. and Mayfield, S.P. 2015. Metabolic engineering of eukaryotic microalgae: Potential and challenges come with great diversity. Frontiers in Microbiology, 6, 1376.

Hegewald, E. and Hanagata, N. 2000. Phylogenetic studies on Scenedesmaceae (Chlorophyta). Algological Studies, 100: 29-49.

A.M. Rajalakshmi et al.

Hena, S., Fatihah, N., Tabassum, S. and Ismail, N. 2015. Three stage cultivation process of facultative strain of Chlorella sorokiniana for treating dairy farm effluent and lipid enhancement. Water Research, 80: 346-356.

Hu, J., Nagarajan, D. and Zhang, Q. 2018. Heterotrophic cultivation of microalgae for pigment production: A review. Biotechnology Advance, 36, 54-67.

Jaiswar, S., Balar, N., Kumar, R., Patel, M.K. and Chauhan, P.S. 2017. Morphological and molecular characterization of newly isolated microalgal strain Neochloris aquatica SJ-1 and its high lipid productivity. Biocatalysis and Agricultural Biotechnology, 9: 108-112.

Leyva-Díaz, J.C., Monteoliva-García, A., Martín-Pascual, J., Munio, M.M., García-Mesa, J.J. and Poyatos, J.M. 2020. Moving bed biofilm reactor as an alternative wastewater treatment process for nutrient removal and recovery in the circular economy model. Bioresource Technology, 299: 122631.

Miranda, J.R., Passarinho, P.C. and Gouveia, L. 2012. Pre-treatment optimization of Scenedesmus obliquus microalga for bioethanol production. Bioresource Technology, 104: 342-348 Molazadeh, M., Ahmadzadeh, H., Pourianfar, H.R., Lyon, S. and Rampelotto, P.H. 2019. The use of microalgae for coupling wastewater treatment with CO2biofixation. Frontiers in Bioengineering and Biotechnology, 7: 42.

Nayak, M., Karemore, A. and Sen, R. 2016. Sustainable valorization of flue gas CO2 and waste water for the production of microalgal biomass as a biofuel feedstock in closed and open reactor systems. RSC Advances, 6(94): 91111-91120.

Nikhil, B., Adhyaru, D. and Thakor, P. 2012. Production of xylanase by Aspergillus flavus FPDN1 on pearl millet bran: Optimization of culture conditions and application in bioethanol production. International Journal of Research in Chemistry and Environment, 2(3): 204-210.

Perazzoli, S., Bruchez, B.M., Michelon, W., Steinmetz, R.L., Mezzari, M.P., Nunes, E.O. and da Silva, M.L. 2016. Optimizing biomethane production from anaerobic degradation of Scenedesmus spp. biomass harvested from algae-based swine digestate treatment. International Biodeterioration & Biodegradation, 109: 23-28.

Sanjivkumar, M., Silambarasan, T., Balagurunathan, R. and Immanuel, G. 2018. Biosynthesis, molecular modeling and statistical optimization of xylanase from a mangrove associated actinobacterium Streptomyces variabilis (MAB3) using Box-Behnken design with its bioconversion efficacy. International journal of biological macromolecules, 118: 195-208.

Sanjivkumar, M., Silambarasan, T., Palavesam, A. and Immanuel, G. 2017. Biosynthesis, purification and characterization of β-1, 4-xylanase from a novel mangrove associated actinobacterium Streptomyces olivaceus (MSU3) and its applications. Protein Expression and Purification, 130: 1-12.

Saranya, D. and Shanthakumar, S. 2019. Green microalgae for combined sewage and tannery effluent treatment: Performance and lipid accumulation potential. Journal of Environmental Management, 241: 167-178.

Selvan, S.T., Govindasamy, B., Muthusamy, S. and Ramamurthy, D. 2019. Exploration of green integrated approach for effluent treatment through mass culture and biofuel production from unicellular alga, Acutodesmus obliquus RDS01. International Journal of phytoremediation, 21(13): 1305-1322.

Selvan, S.T., Velramar, B., Ramamurthy, D., Balasundaram, S. and Sivamani, K. 2020. Pilot scale wastewater treatment, CO2 sequestration and lipid production using microalga, Neochloris aquatica RDS02. International Journal of Phytoremediation, 22(14): 1462-1479.

Shah, Z., Cataluña Veses, R. and Silva, R.D. 2016. GC-MS and FTIR analysis of bio-oil obtained from freshwater algae (Spirogyra) collected from freshwater. International Journal of Environmental & Agriculture Research, 2(2): 134-141.

Shankar, A.M., Henciya, S. and Malliga, P. 2013. Bioremediation of tannery effluent using fresh water cyanobacterium Oscillatoria annae with coir pith. International Journal of Environmental Sciences, 3(6): 1881-1890.

Biosorption of heavy metals by microalgae 37

Sharma, M., Hazra, S. and Basu, S. 2017. Kinetic and isotherm studies on adsorption of toxic pollutants using porous ZnOSiO2 monolith. Journal of Colloid Interface Science, 504: 669- 679.

Shashirekha, V., Sridharan, M.R. and Swamy, M. 2008. Biosorption of trivalent chromium by free and immobilized blue green algae: kinetics and equilibrium studies. Journal of Environmental Science and Health Part A, 43(4): 390-401.

Silambarasan, T.S., Bajwa, K. and Dhandapani, R. 2017. Optimization and mass culture of Acutodesmus obliquus RDS01 under open phototrophic pond cultivation for enhancing biodiesel production. Biofuels, 8(2): 243-252.

Sindhu, R., Binod, P., Janu, K.U., Sukumaran, R.K. and Pandey, A. 2012. Organosolvent pretreatment and enzymatic hydrolysis of rice straw for the production of bioethanol. World Journal of Microbiology and Biotechnology, 28(2): 473-483.

Soeprobowati, Tri Retnaningsih, and Riche Hariyati. 2013. Bioaccumulation of Pb, Cd, Cu, and Cr by Porphyridium cruentum (SF Gray) Nägeli. International Journal of Marine Science, 3(27): 212-218.

Stanley Abraham, L. and Swamy. M. 2001. Role of cyanobacteria in removal of chromium from tannery effluents. pp, 24-47. In: Seminar on Innovations in Leather Science and Technology, Foundation Day, CLRI, Chennai, Tamil Nadu, India.

Thamizhiniyan, P., Sivakumar, P.V., Lenin, M. and Sivaraman, M. 2009. Sugar mill effluent toxicity in crop plants. Journal of Phytology, 1(2): 68-74.

Varela, J.C., Pereira, H., Vila, M. and León, R. 2015. Production of carotenoids by microalgae: achievements and challenges. Photosynthesis Research, 125(3): 423-436.

Wang, H., Ji, C., Bi, S., Zhou, P., Chen, L. and Liu, T. 2014. Joint production of biodiesel and bioethanol from filamentous oleaginous microalgae Tribonema sp. Bioresource Technology, 172: 169-173.

Wang, J., Liu, G., Liu, H. and Lam, P. K. S. 2017. Multivariate statistical evaluation of dissolved trace elements and a water quality assessment in the middle reaches of Huaihe River, Anhui, China. Science of the Total Environment, 583: 421–431.

Wang, L., Chen, M., Li, J., Jin, Y., Zhang, Y. and Wang, Y. 2020. A novel substitution-based method for effective leaching of chromium (III) from chromium-tanned leather waste: The thermodynamics, kinetics and mechanism studies. Waste Management, 103: 276-284.

Wolf, E.W. and Peel, D.A. 1988. Concentrations of cadmium, copper, lead and zinc in snow from near Dye 3 in South Greenland. Annals of Glaciology, 10: 193-197.

Wollmann, F., Dietze, S., Ackermann, J.U., Bley, T., Walther, T., Steingroewer, J. and Krujatz, F. 2019. Microalgae wastewater treatment: Biological and technological approaches. Engineering in Life Sciences, 19(12): 860-871.

Worku, A. and Sahu, O. 2014. Reduction of heavy metal and hardness from ground water by algae. Journal Applied and Environmental Microbiology, 2(3): 86-89.

Published

2023-11-16

How to Cite

BIOSORPTION OF HEAVY METALS FROM TANNERY EFFLUENTS BY USING GREEN UNICELLULAR MICROALGA, Tetradesmus obliquus RDRL01 . (2023). Applied Biological Research, 24(1), 28–37. https://doi.org/10.48165/