BIOSURFACTANT PRODUCTION BY Pseudomonas sp. ISOLATED FROM  HOSPITAL CLINICAL SAMPLES

Muhammad Musthafa Poyil; Sasikumar Palsamy; Ponmurugan Karuppiah

doi:10.48165/

Authors

Muhammad Musthafa Poyil Department of Basic Medical Sciences, College of Medicine, Prince Sattam bin Abdul Aziz University, Al-Kharj, 11942 (Kingdom of Saudi Arabia)
Sasikumar Palsamy Department of Microbiology, The Madura College, Madurai, Tamilnadu (India)
Ponmurugan Karuppiah Department of Botany and Microbiology, College of Science, King Saud University, P. Box 2455, Riyadh 11451 (Kingdom of Saudi Arabia)

DOI:

https://doi.org/10.48165/

Keywords:

Bioremediation, biosurfactant, crude oil degradation, Pseudomonas

Abstract

Biosurfactants are amphiphilic bioactive compounds synthesized extra cellularly by various microbes from various substrates like sugar, oil and other waste materials. In present study a biosurfactant producing bacterium was isolated from clinical samples and characterized morpho biochemically. The isolated bacterium was tentatively identified as Pseudomonas sp. and evaluated for its oil degrading ability. The growth and biomass production by the bacterium was optimized using crude oil at different concentrations at variable incubation periods. The maximum growth was observed after 8 days of incubation on minimal medium containing 20% crude oil while maximum biomass (0.1 g) was noticed after 10 days incubation on basal mediumcontaining 25% crude oil. Based on Rf e biosurfactant produced was of rhaminolipid type I with he isolated bacterium can be employed in bioremediation processes.

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