EVALUATION OF CYTOTOXIC PROPERTIES OF SOME DENTAL MATERIALS Zbigniew Raszewski*

Authors

  • Zbigniew Raszewski Present address: Wyższa Szkoła Menedżerska Bialystok, Sobieskiego 3, 15-013 Bialystok (Poland)

DOI:

https://doi.org/10.48165/

Keywords:

Acrylic resin, alginate impression material, cytotoxicity, zinc phosphate cement

Abstract

In this study we compared the cytotoxic properties of several dental  materials on cell cultures. Six products chosen for the study were alginate  impression material, zinc phosphate cement, tissue conditioner for  temporary denture soft relining, acrylic temporary crown and bridge  material, heat curing acrylic resin and acrylic teeth. The samples were tested  forcytotoxic properties by MTT test on Vero CCL-81 (ATCC) cell line. The  most cytotoxic potential was observed for zincphosphate cement sample  (cell survival, 5%) and highest biocompatibility for thermally polymerized  acrylic resin (>70%). On exposure of dental materials to cytotoxic  properties it did not completely cross out the product, being developed as a  Medical Device. However, it requires further appropriate testing.  

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References

Angelov, G.B. 2015. Systematic relationships among four taxa of genus Melica (Poaceae) in Bulgaria: Isoenzymatic survey. Phytologia Balcanica, 21: 155-159.

Abe, Y., Ueshige, M., Takeuchi, M., Ishii, M. and Akagawa Y. 2003. Cytotoxicity of antimicrobial tissue conditioners containing silver-zeolite. International Journal of Prosthodontic, 16: 141- 144.

Behr, M., Rosentritt, M., Wimmer, J., Lang, R., Kolbeck, C., Bürgers, R. and Handel G. 2003. Self adhesive resin cement versus zinc phosphate luting material: A prospective clinical trial begun. Dental Materials, 25: 601-604.

Boraldi, F., Coppi, Ch., Bortolini, S., Consolo, U. and Tiozzo, R. 2009. Cytotoxic evaluation of elastomeric dental impression materials on a permanent mouse cell line and on a primary human gingival fibroblast culture. Materials, 2: 934-944.

Diemer, F., Stark, H., Helfgen, E.H. 2021. In vitro cytotoxicity of different dental resin-cements on human cell lines. Journal of Materials Science: Materials in Medicine, 32: 4-11. Faggion, C. 2012. Guidelines for reporting pre-clinical in vitro studies on dental materials. The Journal of Evidence-Based Dental Practice, 12: 182-189.

Goiato, M.C., Freitas, E., dos Santos, D., de Medeiros, R. and Sonego, M. 2015. Acrylic resin cytotoxicity for denture base – Literature review. Advanced Clinical Experimental Medicine, 24: 679-686.

Haider, Y., Dimashkieh, M. and Rayyan, M. 2017. Survey of dental materials used by dentists for indirect restorations in Saudi Arabia. The International Journal of Prosthodontics, 30: 83-85. Koosha, S., Nematolahi, F., Torshabi, M. Tabatabaei, F., Borzoo, Y. and Vatandoost, A.H. 2016. In vitro cytotoxicity of two categories of dental cements. Journal of Research in Dental and Maxillofacial Sciences, 1: 28-35.

Lee, H.L., Wang, R.S., Hsu, Y.C., Chuang, C.C., Chan, H.R., Chiu, H.C., Wang, Y.B., Chen, K.Y. and Fu, E. 2018. Antifungal effect of tissue conditioners containing poly (acryloyloxyethyl

Cytotoxic properties of some dental materials 297

trimethyl ammonium chloride)-grafted chitosan on Candida albicans growth in vitro. Journal of Dental Sciences, 13: 160-166.

Mahasti, S., Sattari, M., Romoozi, E. and Akbar-Zadeh Baghban, A. 2011. Cytotoxicity comparison of Harvard zinc phosphate cement versus Panavia F2 and Rely X Plus resin cements on rat L929-fibroblasts. Cell Journal, 13: 163-168.

Mallikarjuna, A.V. 2014. Cytotoxicity of acrylic resin: A review. IOSR Journal of Dental and Medical Sciences, 13: 7-9.

Marvin, J.C., Gallegos, S.I., Parsaei, S. and Rodrigues, D.C. 2018. In vitro evaluation of cell compatibility of dental cements used with titanium implant components. Journal of Prosthodontics, 1: 1-8.

Moharamzadeh, K., Brook, I.M. and Van Noort, R. 2009. Biocompatibility of resin-based dental materials. Materials, 2: 514-548.

Narvekar, A., Ramamoorthi, M. and Sivakumar A. 2015. Cytotoxic effects of restorative biomaterials on dental pulp – A systematized review. World Journal of Pharmacy and Pharmaceutical Sciences, 4: 239-266.

Nishijima, M., Hashimoto, Y. and Nakamura, M. 2002. Cytocompatibility of new phthalate ester free tissue conditioners in vitro. Dental Materials Journal, 21: 118-132.

Pithon, M.M., Dos Santos, R.L., Martins, F.O. and Romanos, M.T.V. 2010. Cytotoxicity of dental alginates. International. Journal of Odontostomatology, 4: 303-308.

Pithon, M.M, Santos, R.L., Martins, F.O. and Romanos, M.T.V. 2012. Cytotoxicity of alginate for orthodontic use. Dental Press Journal of Orthodontics, 17: 21.e1-5.

Priyaranjan, K.K., Thota, S.K. and Sayed, A.J. 2020 An in vitro assessment of cytotoxicity of polyvinyl siloxane, polyether and polyvinyl ether silicone on NIH/3T3 cells. Journal of Contemporary Dental Practice, 21:1262-1265.

Rashid, H., Sheikh, Z. and Vohra, F. 2015. Allergic effects of the residual monomer used in denture base acrylic resins. European Journal of Dentistry, 9: 614-619.

Rueggeberg, F.A. 2002. From vulcanite to vinyl, a history of resins in restorative dentistry. Journal of Prosthetic Dentistry, 87: 364-379.

Sahabi, M., Sattari, M., Romoozi, E. and Akbar-Zadeh Baghban, A. 2011. Cytotoxicity comparison of Harvard zinc phosphate cement versus Panavia F2 and Rely X Plus resin cements on rat L929-fibroblasts. Cell Journal (Yakhteh), 13: 163-168.

Schweikl, H., Spagnuolo, G. and Schmalz, G. 2006. Genetic and cellular toxicology of dental resin monomers. Journal of Dental Research, 85: 870-877.

Tay, L.Y., Herrera, D.R., Campos, C., Quishida, C., Zeppone, I., Janaina, C. and Jorge, H. 2012. Effect of water storage and heat treatment on the cytotoxicity of soft liners. Geriodontology, 29: e275-e280.

Tillberg, A., Järvholm, B. and Berglund, A. 2008. Risks with dental materials. Dental Materials, 24: 940-943.

Wassmann, T., Schubert, A. and Malinski, F. 2020. The antimicrobial and cytotoxic effects of a copper-loaded zinc oxide phosphate cement. Clinical Oral Investigation, 24: 3899-3909.

Published

2023-11-16

How to Cite

EVALUATION OF CYTOTOXIC PROPERTIES OF SOME DENTAL MATERIALS Zbigniew Raszewski* . (2023). Applied Biological Research, 23(3), 293–297. https://doi.org/10.48165/