EFFECT OF GAMMA IRRADIATION ON SOME SELECTED VARIETIES OF INDIAN MUSTARD (Brassica juncea L.)
DOI:
https://doi.org/10.48165/Keywords:
Ethyl methane sulfonate, gamma radiation, Indian mustard, mutagen, physiological traitsAbstract
An experiment was conducted to study the impact of gamma (γ) rays on some quantitative traits such as germination and survival of seeds; morpho physiological and yield attributes of Indian mustard (Brassica juncea L.). Two varieties of B.juncea viz., ‘Pusa Bold’ and ‘Kranti’, were used in the present study. The seeds of Brassica were irradiated witvarious doses of γ ray (900, 1000, 1100 and 1200 Gy) and a combination treatment with ethyl methane sulfonate (1000 Gy + 2% EMS). The control seeds were not exposed to either radiation or EMS. The results revealed that dosesof γ radiation altered all the studied traits for both the tested varieties. Increasing doses of γ-radiation were responsible for decreasing the seed germination, plant survival, plant height, number of siliqua plant-1, seed siliqua-1dry weight of whole plant, SPAD value, weight of dry husk plant-1, leaf area and photosynthetic rate. Plants in M1 generation indicated dose dependent physiological changes of genotype as compared to the control. Thisalternation of each traits indicated that γ-radiation has some effect on Brassica seeds which might be utilized asmutant for future variety development programme.
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Abdel-Hady, M.S., Okasha, E.M., Soliman, S.S.A. and Talaat, M. 2008. Effect of gamma radiation and gibberellic acid on germination and alkaloid production in Atropa belladonna. Australian Journal of Basic and Applied Science, 2(3): 401-405.
Anjum, T. and Bajwa, R. 2005. Importance of germination indices in interpretation of allele chemical effects. International Journal of Agriculture and Biology. 7: 417-419. Anbarasan, K., Rajendran, R. and Sivalingam, D. 2015. Studies on the mutagenic effectiveness and efficiency of gamma rays, EMS and combined treatment in sesame (Sesamum indicum L.) var. TMV3. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(4): 589-595
Asare, T.A., Mensah, F., Acheampong, S., Bediako, E.A. and Armah, J. 2017. Effects of gamma irradiation on agro-morphological characteristics of okra (Abelmoschus esculentus L. Moench.). Advances in Agriculture, 3:1-7 [https://doi.org/10.1155/2017/2385106].
Ashraf, M. 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology Advances, 27(1): 84-93.
Ashraf, M., Cheema, A.A., Rashid, M. and Zia-ul-Qamar. 2003. Effect of gamma rays on M1 generation in basmati rice. Pakistan Journal of Botany, 35(5): 791-795.
Chandrashekar, K., Somashekarappa, H. and Souframanien, J. 2013. Effect of gamma irradiation on germination, growth, and biochemical parameters of Terminalia arjuna Roxb. Radiation Protection and Environment, 36:38-44.
Channaoui, S., Labhilili, M., Mouhib, M., Mazouz, H., Fechtali, M.E. and Nabloussi, A. 2019. Development and evaluation of diverse promising rapeseed (Brassica napus L.) mutants using physical and chemical Mutagens, Oilseeds and Fats, Crops and Lipids, 26: 35 [https://doi.org/10.1051/ocl/2019031].
Chaudhuri, S.K. 2002. A simple and reliable method to detect gamma-irradiated lentil (Lens culinaris Medik.) seeds by germination efficiency and seedling growth test. Radiation Physics and Chemistry, 64:131-136.
Deshmukh, P.D. and Malode, S.N. 2018. Effects of gamma radiation on seed germination, plant survival and growth characteristics in Dianthus caryophyllus var. Chabaud. Journal of Global Biosciences, 7(3): 5403-5410.
Dubey, A.K., Yadav, J.R. and Singh, B. 2007. Studies on induced mutations by gamma irradiation in okra [Abelmoschus esculentus (L.) Monch]. Progressive Agriculture, 7(1): 46-48. El-Ashry, A.I., Zagloul, M.A. and Ghait, E.A.A. 1992. Effect of gamma radiation on growth and flowering of Lathyrus odoratus L., Bulletin Suez Canal University Applied Science, 1: 506- 521.
El-Mahrouk, M.E.M. 2000. Induction of Genetic Variability in Gomphrena globosa, L. Plant by Gamma Rays. M.Sc. Thesis, Faculty of Agriculture, Tanta University, Kafr EI-Sheikh, Egypt. Girija, M., Dhanavel, D. and Gnanamurthy, S. 2013. Gamma rays and EMS induced flower color and seed mutants in cowpea (Vigna unguiculata L. Walp), Advances in Applied Science Research, 4(2): 134-139.
Effect of γ-radiation on some varieties of Indian mustard 291
Gunckel, J.E. and Sparrow, A.H. 1961. Ionizing radiation: biochemical, physiological and morphological aspects of their effects on plants. pp: 555-611. In: Encyclopedia of Plant Physiology (ed. W. Ruhland), Springer-Verlag, Berlin, Germany.
Haris, A. and Jusoff, K. 2013. Gamma ray radiation mutant rice on local aged dwarf. Middle East Journal of Scientific Research. 15(8): 1160-1164.
Irfaq, M. and Nawab, K. 2001. Effect of gamma irradiation on some morphological characteristics on three wheat (Triticum aestivum L.) cultivars. Journal of Biological Science, 1(10): 935- 937.
Jagajanantham, N., Dhanavel, D., Pavadaiand, P. and Chidambaram, A.A. 2012. Growth and yield parameters using gamma rays in bhendi [Abelmoschus esculentus (L.) Moench] var. arkaanamika. International Journal of Research in Plant Science, 2(4): 56-58.
Jan, S., Parween, T. and Siddiqi, T. 2012. Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products. Environmental Reviews, 20:17-39.
Julia, T. Renuka, T., Nanita, H. and Jambhulkar, S. 2018. Mutagenic effectiveness and efficiency of gamma rays in Indian mustard (Brassica juncea L. Czern & Coss). International Journal of Current Microbiology and Applied Sciences, 7(3): 3376-3386.
Khalil, S.K., Rehman, S., Afridi, K. and Jan, M.T. 1986. Damage induced by gamma irradiation in morphological and chemical characteristics of barley. Sarhad Journal of Agriculture, 2: 45- 54.
Khan, W.M., Shah, S.Z., Khan, M.S., Islam, Z.U., Ali, S., Hussain, F., Irshad, M. and Zahid M. 2014. Effects of gamma radiations on some morphological and biochemical characteristics of Brassica napus L. (variety Altex). International Journal of Biosciences, 4(10): 36-41.
Kim, J.H., Baek, M.H., Chung, B.Y., Wi, S.G. and Kim, J.S. 2004. Alterations in the photosynthetic pigments and antioxidant machineries of red pepper (Capsicum annuum L.) seedlings from gamma-irradiated seeds. Journal of Plant Biology, 47: 314-321.
Kiong, A.L.P., Lai, A.G., Hussain, S. and Harun, A.R. 2008. Physiological responses of Orthosiphon stamineus plantlets to gamma irradiation. American Eurasian Journal of Sustainable Agriculture, 2(2): 135-149.
Kovacs, E. and Keresztes, A. 2002. Effect of gamma and UV-B/C radiation on plant cells. Micron, 33(2): 199–210. [doi:10.1016/ S0968-4328(01)00012-9. PMID:11567888].
Kumari, R. and Singh, Y. 1996. Effect of gamma rays and EMS on seed germination and plant survival of Pisum sativum L. and Lens culinaris. Medic. Neo Botanica, 4(1): 25-29. Lamo,K., Bhat, D.J., Kour, K. and Solanki, S.P.S. 2017. Mutation studies in fruit crops: A review. International Journal of Current Microbiology and Applied Sciences, 6(12): 3620-3633. Marcu, D., Damian, G., Cosma, C. and Cristea, V. 2013. Gamma radiation effects on seed germination, growth and pigment content and ESR study of induced free radicals in maize (Zea mays). Journal of Biological Physics, 39 (4):625-634.
Mashev, N., Vassilev, G. and Ivanov, K. 1995. A study of N-allyl N-2 pyridyl thiourea and gamma radiation treatment on growth and quality of peas and wheat. Bulgarian Journal of Plant Physiology, 21(4): 56–63.
Mishra, M.N., Qadri, H. and Mishra, S. 2007. Macro- and micro-mutations, in gamma-rays induced M2 populations of okra (Abelmoschus esculentus (L) Moench). International Journal of Plant Science, 2(1): 44-47.
Mujeeb, K.A. and Greij, J.K. 1976. Growth stimulation in Phaseolus vulgaris L. induced by gamma irradiation of seeds. Biologia Plantarum, 18: 301-303.
Ochatt, S.J., Durieu, P., Jacas, L. and Pontecaille, C. 2001. Protoplast, cell and tissue cultures for the biotechnological breeding of grass peas (Lathyrus sativus L). Lathyrus and Neurolathyrism Newsletter, 2: 35-38.
Preuss, S.B. and Britt, A.B. 2003. A DNA-damage-induced cell cycle checkpoint in Arabidopsis. Genetics, 164(1): 323-334.
Chamim Sultana Ahmed et al.
Pushparajan, G., Sanoopand, S. and Harinarayanan M.K. 2014. Effect of gamma rays on yield attributing characters of okra [Abelmoschus esculentus (L.) Moench]. International Journal of Advanced Research, 2(5): 535-540.
Raghava, R.P. and Raghava, N. 1989. Effect of gamma irradiation on fresh and dry weight of plant parts in Physallis L. Geobios, 16(6): 261-264.
Rahimi, M.M. and Bahrani, A. 2011. Effect of gamma irradiation on qualitative and quantitative characteristics of canola Brassica napus L. Middle-East Journal of Scientific Research, 8(2): 519-525.
Ramakrishna, D., Chaitanya, G., Suvarchala, V. and Shasthree, T. 2018. Effect of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of Citrullus colocynthis (L.) Schrad. Journal of Plant Biotechnology, 45(1): 55-62.
Ramesh, H.L., Murthy, V.N.Y. and Munirajappa 2012. Effect of different doses of gamma radiation on growth parameters of mulberry (Morus) variety Kosen. Journal of Applied and Natural Science 4 (1): 10-15.
Saha, P., Raychaudhuri, S.S., Chakraborty, A. and Sudarshan, M. 2010. PIXE analysis of trace elements in relation to chlorophyll concentration in Plantago ovata Forsk. Applied Radiation and Isotoped, 68(3): 444–449.
Salem, N., Jan, S.A., Atif, M.J., Khurshid, H., Khan, S.A., Abdullah, M., Jahanzaib, M., Ahmed, H., Farman-Ullah, S., Iqbal, A., Sameena Naqi, Ilyas, M., Ali, N. and Rabbani, M.A. 2017. Multivariate based variability within diverse Indian mustard (Brassica juncea L.) genotypes. Open Journal of Genetics, 7: 69-83.
Salifu, B. 2015. Effects of Cobalt-60 Gamma Irradiation on Growth and Yield of Pearl Millet (Pennisetum glaucum L.) in the Guinea Savannah Agro-ecological Zone of Ghana, Thesis of Master of Philosophy in Crop Science, University for Development Studies, Ghana. [www.udsspace.uds.edu.gh].
Shah, T.M., Mirza, J.I., Haq, M.A. and Atta, B.M. 2008. Radio sensitivity of various chickpea genotypes in M1 generation I - Laboratory studies. Pakistan Journal of Botany, 40(2): 649– 665.
Shamsi, S.R.A. and Sofajy, S.A. 1980. Effects of low doses of gamma radiation on the growth and yield of two cultivars of broad bean. Environmental and Experimental Botany, 20(1): 87–94. Shamusuzzaman, K.M., Islamand, M.M. and Muwara, B. 2005. Development of an early maturing chickpea variety. Breeding Newsletter and Reviews, 1(23). [Development of an early maturing chickpea variety, BINASOLA-3..|INIS (iaea.org)].
Sharma, B. and Mishra, K. 2007. Micro-mutations for fruit number, fruit length and fruit yield characters in gamma-irradiated generation of ANKUR-40 variety of okra. Abelmoschus esculentus L.) Monech. International Journal of Plant Science, 2(2): 208–211.
Sherif, El. F., Khattab, S., Goniam, E., Salem, N. and Radwan, K. 2011. Effect of gamma irradiation on enhancement of some economic traits and molecular changes in Hibiscus Sabdariffa. Life Science Journal, 8(3): 220-229.
Szarejko, I. and Forster B.P. 2007. Doubled haploidy and induced mutation. Euphytica, 158: 359- 370.
Wi, S.G., Chung, B.Y. and Kim, J.S. 2007. Effects of gamma irradiation on morphological changes and biological responses in plants. Micron, 38(6): 553-564.
Yadav, V. 2016. Effect of gamma radiation on various growth parameters and biomass of Canscorade currens Dalz. International Journal of Herbal Medicine, 4(5): 109-115. Yadav, P., Meena, H.S., Meena, P.D., Kumar, A., Gupta, R., Jambhulkar, S., Rani, R. and Singh, D. 2016. Determination of LD50 of ethyl methane sulfonate (EMS) for induction of mutations in rapeseed-mustard. Journal of Oilseed Brassica, 7(1): 77-82.