IDENTIFICATION OF VOLATILE COMPOUNDS IN FERMENTED MILLET BASED BEVERAGES BY GAS CHROMATOGRAPHY-MASS SPECTROSCOPY
DOI:
https://doi.org/10.48165/Keywords:
Beverages, fermentation, GC-MS, millets, volatile compoundsAbstract
Volatile compounds impart flavour in food and beverages and make the food and food products appealing. The present study involves the development of fermented millet beverages from minor millets viz., foxtail millet, little millet, kodo millet, proso millet and barnyard millet, by using an isolate of Propionibacterium sp. as fermentation agent. The volatile compounds present in the newly developed fermented millet-based beverages were identified using hexane-extracted samples by gas chromatography-mass spectrophotometry (GC-MS). The results revealed thpresence of 21 volatile compounds in foxtail- and proso-millets, 18 compounds in little millet and barnyard millet and 19 compounds in kodo millet. The dominant volatile compounds observed were benzene derivatives, hexadeconoic acid, acetic acid, sitosterol and octadecanoic acid in all five minor millet fermented beverages.
Downloads
References
Akyol, I., Ozcelik, F.G., Karakas-Sen, A., Ozkose, E., Gezginc, Y. and Ekinci, M.S. 2015. Cloning and overexpression of the als, pfla and adhb genes in Streptococcus thermophilus and their effects on metabolite formation. Molecular Biotechnology, 57: 923-930.
Beshkova, D., Simova, E., Frengova, G. and Simov. Z.1998. Production of flavor compounds by yogurt starter cultures. Journal of Industrial Microbiology & Biotechnology, 20: 180-186. Beshkova, D.M., Simova, E.D., Frengova, G.I., Simov, Z.I. and Dimitrov, Z.P. 2003. Production of volatile aroma compounds by kefir starter cultures. International Dairy Journal, 13: 529-535.
Volatile compounds in fermented millet-based beverages 253
Bodyfelt, F.W. and Potter, D. 2008. The Sensory Evaluation of Dairy Products in the Creamed Cottage Cheese. Springer, New York, USA.
Bok, F.A.M.D., Janssen, P.W.M., Bayjanov, J.R., Sieuwerts, S., Lommen, A., Vlieg, J.E.T.V.H. and Molenaar, D. 2011. Volatile compound fingerprinting of mixed-culture fermentations. Applied & Environmental Microbiology, 77: 6233-6239.
Champagne, E.T., Bett-Garber, K.L., McClung, A.M. and Bergman, C. 2004. Sensory characteristics of diverse rice cultivars as influenced by genetic and environmental factors. Cereal Chemistry, 81: 237-243.
Chaves, A.C.S.D., Ruas-Madiedo, P., Starrenburg, M., Hugenholtz, J. and Lerayer. A.L.S. 2011. Impact of engineered Streptococcus thermophilus strains overexpressing glyA gene on folic acid and acetaldehyde production in fermented milk. Brazilian Journal of Microbiology, 34(1): 114-117.
Cheng, H. 2010. Volatile flavor compounds in yogurt: A review. Critical Review of Food Science and Nutrition, 50: 938-950.
Choi, Y., Jeong, H.S. and Lee, J. 2007. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chemistry, 103: 130-138.
Chughtai, M.F.J., Pasha, I., Anjun, F.M. and Nasir, M.A. 2015. Characterization of sorghum and millet with special reference to fatty acid and volatile profile. Turkish Journal of Agriculture Food Science and Technology, 3(7): 515-521.
Cramer, A.C.J., Mattinson, D.S., Fellman, J.K. and Baik, B.K. 2005. Analysis of volatile compounds various types of barley cultivars. Journal of Agricultural Food Chemistry, 53: 7526-7531.
Dan, T. 2017. Profiles of volatile flavour compounds in milk fermented with different proportional combinations of Lactobacillus delbruekii subsp. bulgaricus and Streptococus thermophilis. Molecules, 22: 1633-16337.
Dan, T., Wang, D., Jin, R.L., Zhang, H.P., Zhou, T.T. and Sun, S. 2017. Characterization of volatile compounds in fermented milk using solid-phase micro extraction methods coupled with gas chromatography-mass spectrometry. Journal of Dairy Sciences, 100: 2488-2500.
De Mejia, E.G., and Dia, V.P. 2010. The role of nutraceutical proteins and peptides in apoptosis, angiogenesis, and metastasis of cancer cells. Cancer Metastatis Review, 29: 511-528. Erkus, O., Okuklu, B., Yenidunya, A.F. and Harsa, S.2013. High genetic and phenotypic variability of Streptococcus thermophilus strains isolated from artisanal Yuruk yoghurts. LWT – Food Science and Technology, 58: 348-354.
Fuchs, G. 2008. Anaerobic metabolism of aromatic compounds. Annals of the New York Academy of Sciences, 1125, p. 82-99. [https://doi.org/10.1196/annals.1419.010].
Gezginc, Y., Topcal, F., Comertpay, S. and Akyol, I. 2015. Quantitative analysis of the lactic acid and acetaldehyde produced by Streptococcus thermophilus and Lactobacillus bulgaricus strains isolated from traditional Turkish yogurts using HPLC. Journal of Dairy Science, 98: 1426-1434.
Gibson, B., Aumala, V., Heinio, R.L., Mikkelson, A. and Honkapaa. 2018. Differential evolution of strecker and non-strecker aldehydes during aging of pale and dark beers. Journal of Cereal Science, 83: 130-138.
Guler, M.O. and Stupp. S.I. 2007. A self-assembled nanofiber catalyst for ester hydrolysis. Journal American Chemistry Society, 129: 12082-12083.
Hameed, I.H., Hussain, J., Kareem, M.A. and Nidaa, S. 2015. Identification of five newly described bioactive chemical compounds in methanolic extract of Mentha viridis by using GC-MS. Journal of Pharmacognosy and Phytotherapy, 7(7): 107-125.
Hanafi, E.M., Ramadan, M.M., Kassem, S.S., Kader M.M.A., Kholif, A.M., Award, G.E.A. and Danial E.N. 2014. A novel formulation based on pearl millet for broilers feedings. International Journal of Pharmaceutical Biological and Chemical Sciences, 3(4): 27-30.
P. Sheela et al.
Hill, C., Guarner, F., Reid, G., Gibson, G.R, Merenstein, D.J. and Pot, B. 2014. Expert consensus document: The international scientific association for probiotics and consensus statement on the scope and appropriate use of the term probiotic. Nature Review Gastroenterology and Hepatology, 11: 506-514.
Janes, D., Kantar, D., Kreft, S. and Prosen, H. 2009. Identification of buckwheat (Fagopyrum esculentum Moench) aroma compounds with GC-MS. Food Chemistry, 112(1): 120-124. Jingke L.., Shanohui Li., Aixia Zhang., Wei Zhao., Yingying, L. and Yuzong Zhang. 2016. Volatile
profiles of 13 foxtail millet commercial cultivars (Setaria italica Beauv.) from China. Cereal Chemistry, 94(2): 170-176.
Karthika, S., Ravishankar, M., Mariajancyrani, J. and Chandramohan, G. 2013. Study on phyto chemical from Moringa oleifera leaves. Asian journal of Plant Science and Research, 3(4): 63-69.
Khan, I.U., Meena, R.C., Raiger, P.R., Rathore, B.S., Satyavathi, C.T. and Singh, B. 2017. Evaluation and identification of volatile bioactive compounds in methanol extract of pearl millet genotypes by GC-MS. International Journal of Pure and Applied Bioscience, 5(2): 526-531.
Liang, H.Z. 2012. In situ real-time monitoring of volatile metabolites of fermented milk by dynamic headspace sampling-atmospheric pressure ionization mass spectrometry. Food Science, 33: 307-310.
Liu, J., Tang, Y. and Zhao, W. 2012. Determination of the volatile composition in brown millet, milled millet and millet bran by gas chromatography-mass spectrometry. Molecules, 17: 2271-2282.
Liu, T.T. and Yang, T.S. 2011. Effects of an industrial milling process on change of headspace volatiles in Yihchuan aromatic rice. Cereal Chemistry, 88: 137-141.
Ludwing, I.A., Clifford, M.N., Lean, M.E.J., Ashihara, H. and Crozier, A. 2014. Coffee: Biochemistry and potential impact on health. Food and Function, 5(8): 1695-1717. Mathew, O.P. 2011. Apnea of prematurity: Pathogenesis and management strategies. Journal of Perinatology, 31: 302-310.
Meira, S.M.M., Daroit, D.J. and Helfer, V.E. 2012. Bioactive peptides in water soluble extract of ovine cheese from southern Brazil and Uruguay. Food Research International, 48: 322-329. Ott, A., Fay, L.B. and Chaintreau, A. 1997. Determination and origin of the aroma impact compounds of yogurt flavor. Journal of Agriculture and Food Chemistry, 45: 850-858. Pan, D.D., Wu, Z., Peng, T., Zeng, X.Q. and Li, H. 2014. Volatile organic compounds profile during milk fermentation by Lactobacillus pentosus and correlations between volatiles flavor and carbohydrate metabolism. Journal of Dairy Science, 97: 624-631.
Perna, A., Intagllietta, I., Simonetti, A. and Gambacorta, E. 2013. Effects of genetic type and casein halotype on antioxidant activity of yogurts during storage. Journal of Dairy Science, 96: 1-7.
Settachaimongkon, S., Nout, M.J., Antunes Fernandes, E.C., Hettinga, K.A., Vervoort, J.M., Van Hooijdonk, T.C., Zwietering, M.H., Smid, E.J. and Van Valenberg H.J. 2014. Influence of different proteolytic strains of Streptococcus thermophilus in co-culture with Lactobacillus delbrueckii subsp. bulgaricus on the metabolite profile of set-yoghurt. International Journal of Food Microbiology, 177: 29-36.
Sheela, P., Kanchana, S., Uma Maheshwari, T. and Hemalatha, G. 2018. Optimization of parameters for the extraction of millet milk for product development. Research Journal of Agricultural Sciences, 9(6): 1345-1349.
Singh, P. and Raghuvanshi, R.S. 2012. Finger millet for food and nutritional security. African Journal of Food Science, 6(4): 77-84.
Starrenburg, M.J. and Hugenholtz, J. 1991. Citrate fermentation by Lactococcus and Leuconostoc spp. Applied and Environmental Microbiology, 57: 3535-3540.
Volatile compounds in fermented millet-based beverages 255
Wongpornchai, S., Dumria, K., Jongkaewwattanana, S. and Siri, B. 2004. Effects of drying methods and storage time on the aroma and milling quality of rice (Oryza sativa L.) cv. Khao Dawk Mali 105. Food Chemistry, 87: 407-414.
Zha, M., Yu, J., Zhang, Y., Wang, H., Bai, N., Qin, Y., Liangliang, D., Liu, W., Zhang, H. and Bilige, M. 2015. Study on Streptococcus thermophilus isolated from Qula and associated characteristic of acetaldehyde and diacetyl in their fermented milk. Journal of General and Applied Microbiology, 61: 50-56.