GENETIC DIVERSITY OF Melica POPULATIONS AND THEIR STABILITY  IN ECOLOGICAL LANDSCAPE

Abdolrahman Rahimian Boogar; Soheila Gholami; Nasim Forghani; Elham Bagheri Abyaneh; Seyed Mohsen Hesamzadeh Hejazi

doi:10.48165/

Authors

Abdolrahman Rahimian Boogar Department of Horticultural Science and Landscape Engineering, Faculty of Agriculture, University of Zabol, Zabol (Iran)
Soheila Gholami Department of Medicine Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, (Iran)
Nasim Forghani Department of Cell and Molecular Biology, University of Tehran, Tehran (Iran)
Elham Bagheri Abyaneh Department of Biology, University of Payame-Noor, Tehran (Iran)
Seyed Mohsen Hesamzadeh Hejazi Research Institute of Forests and Rangelands, Tehran (Iran)

DOI:

https://doi.org/10.48165/

Keywords:

Climate, cytogenetic, diversity, natural habitat, population

Abstract

Melica grass has effective role in ecological landscape and its stability is affected by population genetics. Main purpose of this study was to investigate the relation and distance of Melica populations genetically that are grown in unlike topographic and climatic conditions. Karyotyping and leaf storage protein SDS PAGE were employed to investigate the genetic diversity of 10 populations of 2 species of Melica viz., M. persica and M. jacquemontii as major plants in ecological landscape of Esfahan, Alborz, Ghom, Golestan, Kerman and Yazd habitats in Iran. The result of karyotyping showed that all the populations have similar chromosomenumber(2n=2x=18), but cytogenetic parameters exhibited significant differences in all the populations. Ward similarity analysis revealed tions can be clustered into 4 groups. SDSPAGE of leaf protein indicated that the population of M. persica ‘21856’ had highest protein fragment while the lowest fragments belonged to M. jacquemontii [7683 and 10506]. Jaccard similarity coefficient revealed genetic variation in all the studied populations of M. persica, but M. jacquemontii showed no variation. M. persica had high diversity and more stability in ecological landscape and these results suggest that the species of M. jacquemontii is a subspecies of M. persica.

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References

Angelov, G.B. 2015. Systematic relationships among four taxa of genus Melica (Poaceae) in Bulgaria: Isoenzymatic survey. Phytologia Balcanica, 21: 155-159.

Averello, V., Kubik, C., Vaiciunas, J., Meyer, W.A., Bonos, S.A. and Honig, J.A. 2015. Genetic diversity of tall fescue (Lolium arundinaceum (Screb.) Darbysh.) cultivars using microsatellite (SSR) markers. In: Annual Meeting of the American Society of Agronomy/the Crop Science Society of America/ the Soil Science Society of America, 15-18 November, 2015, Minneapolis, USA.

Bagheri Abyaneh, E., Hesamzadeh, S.M., Gholami, S. and Bakhshi Khaniki, G. 2017. Study on the cytotaxonomy of some species of the genus Festuca (Poaceae). Iranian Journal of Science and Technology, Transactions A: Science, 42: 1689-1696.

Cântar, I.C. and Dincă, M. 2017. Two genus of plants from Poaceae family (Melica and Eragrostis) existing in “Alexandru Beldie” herbarium of I.N.C.D.S. Bucharest. Journal of Horticulture, Forestry and Biotechnology, 21: 68-76.

Clayton, W.D. and Renvoize, S.A. 1986. Genera Graminum. Her Majesty’s Stationery Office, London, UK.

Cuyeu, R., Rosso, B., Pagano, E., Soto, G., Fox, R. and Ayub, N.D. 2013. Genetic diversity in a world germplasm collection of tall fescue. Genetics and Molecular Biology, 36(2): 237-242. Dallwitz, M.J. Watson, L. 2005. The Grass Genera of the World: Descriptions, Illustrations, Identification and Information Retrieval including Synonyms, Morphology, Anatomy, Physiology, Phytochemistry, Cytology, Classification, Pathogens World and Local Distribution, and References. 28th Version. November. [http://delta-intkey.com]. El-Ghamery, A., Morsy, M. and Abu-Shahba, M. 2016. Chromosome count and karyotype study on endemic species Silene schimperiana Boiss. (Caryophyllaceae), Sinai, Egypt. Taeckholmia, 36: 102-114.

Eliáš Jun, P., Dítě, D., Dítě, Z. and Eliašová, M. 2017. Melica altissima in Slovakia, vanishing grass species of forest ecotones. Thaiszia Journal of Botany, 27(2): 117-128.

Falistocco, E., Marconi, G. and Falcinelli, M. 2013. Comparative cytogenetic study on Trifolium subterraneum (2n = 16) and Trifolium israeliticum (2n = 12). Genome, 56: 307-313. Genç, I., Özhatay, N. and Cevri, M. 2013. A karyomorphological study of the genus Allium (Sect. Melanocrommyum) from Turkey. Caryologia, 66: 31-40.

Gupta, R.C., Chauhan, H.S., Saggoo, M.I.S. and Kaur, N. 2014. Cytomorphology of some grasses (Poaceae) from Lahaul-Spiti (Himachal Pradesh), India. Biolife, 2: 1234-1247. Hand, M.L., Cogan, N.O. and Forster, J.W. 2012. Molecular characterization and interpretation of genetic diversity within globally distributed germplasm collections of tall fescue (Festuca

Genetic diversity of Melica populations 233

arundinacea Schreb.) and meadow fescue (F. pratensis Huds.). Theoretical and Applied Genetics, 124(6): 1127-1137.

Hesamzadeh, S.M. and Rasuli, M. 2006. Cytogenetic study of some species of Vetch genus (Vicia sp.) in Iran. Iranian Journal of Agriculture Science, 37: 213-225.

Honig, J.A., Bonos, S.A. and Meyer, W.A. 2010. Isolation and characterization of 88 polymorphic microsatellite markers in Kentucky bluegrass (Poa pratensis L.). Hortscience, 45: 1759-1763. Huziwara, Y. 1962. Karyotype analysis in some genera of Compositae. Further studies on the chromosome of Aster. American Journal of Botany, 49: 116-119.

Koal, K.K. and Gohil, R.N. 1987. SOCGI plant chromosome number reports - IV. Journal of Cytology and Genetics, 21: 161-162.

Konichenko, E., Selyutina, I., Dorogina, V. and Sandanov, D. 2014. Karyotype studies endemic plant species Astragalus sericeocanus Gontsch. (Fabaceae) around lake Baikal, Siberia. Caryologia, 67: 172-177.

Kumari, K. and Saggoo, M. 2016. Male meiosis in two morphotypes of Melica persica Kunth (Poaceae) from Himachal Pradesh, India. Cytologia, 81: 403-408.

Kursat, M., Emre, I., Gedik, O. and Kiran, Y. 2018. Karyological reports for some Salvia taxa from Turkey, Caryologia, 71(2): 120-127.

Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.

Levan, A.K., Fredga, K. and Sandberg, A.A. 1964. Nomenclature for centromic position on chromosomes. Hereditas, 52: 201-220.

Li, Y.P., Zhang, X., Wu, W.T., Miao, S.X. and Chang, J.L. 2015. Chromosome and karyotype analysis of Hibiscus mutabilis f. mutabilis. Frontiers in Life Science, 8(3): 300-304. Liang, G. and Chen, H. 2015. Scaling chromosomes for an evolutionary karyotype: A chromosomal tradeoff between size and number across woody species. PLoS ONE, 10: e0144669. Lima, M.G.F., Silveira, G.L., Techio, V.H. and Andrade-Vieira, L.F. 2019. Effects of three antimitotic agents on karyotype of Allium cepa L. and Lactuca sativa L.: Two plant model species for cytogenotoxic assessments. South African Journal of Botany, 125: 244-250. Long, R. and Anderson, J. 2010. Establishing Hedgerows on Farms in California, Oakland. Agriculture and Natural Resources Publication 8390, University of California, California, USA.

Medeiros-Neto, E., Nollet, F., Moraes, A. and Felix, L. 2017. Intrachromosomal karyotype asymmetry in Orchidaceae. Genetics and Molecular Biology, 40: 610-619.

Mejia-Saules, T. and Bisby, F.A. 2003. Silica bodies and hooked papillae in lemmas of Melica species (Gramineae: Pooideae). Botanical Journal of the Linnean Society, 143: 447-463. Meyer, W.A., Hoffman, L. and Bonos, S.A. 2017. Breeding cool-season turfgrass cultivars for stress tolerance and sustainability in a changing environment. International Turfgrass Society Research Journal, 13: 1-3.

Niklfeld, H. and Schratt-Ehrendorfer, L. 1999. Rote ListegefährdeterFarn– und Blütenpflanzen (Pteridophyta und Spermatophyta) Österreichs. 2. Fassung. In: Rote Listen gefährdeter PflanzenÖsterreichs, 2. Auflage. Grüne Reihe Bundesmin. (ed. H. Niklfeld), Umwelt Jugend Familie (Wien) 10: 33-130.

Porensky, L., Vaughn, K. and Young, T. 2012. Can initial interspecific spatial aggregation increase multi-year coexistence by creating temporal priority? Ecological Applications, 22: 927-936. Prieto, I., Violle, C., Barre, P., Durand, J.L., Ghesquiere, M. and Litrico, I. 2015. Complementary effects of species and genetic diversity on productivity and stability of sown grasslands. Nature Plants, 1: 1-5.

Raggi, L., Bitocchi, E., Russi, L., Marconi, G., Sharbel, T.F., Veronesi, F. and Albertini, E. 2015. Understanding genetic diversity and population structure of a Poa pratensis worldwide collection through morphological, nuclear and chloroplast diversity analysis. PloS ONE, 10 (4): e0124709.

Abdolrahman Rahimian Boogar et al.

Reeves, A. and Tear, J. 2000. Micro-Measure for Windows. version 3.3. [http://www.colostate.Edu/- depts/biology/micromeasure].

Riasat, M. and Sadeghian, S. 2019. Karyology study of ten Trifolium species in Fars province. Journal of Genetic Resources, 5: 136-142.

Romero Zarco, C. 1986. A new method for estimating Karyotype asymmetry. Taxon, 36: 526-530. Sacramento Stomwater Quality Partnership (SSQR). 2003. River-Friendly Landscape Guidelines. [https://www.waterboards.ca.gov/academy/courses/eco-landscape].

Samaropoulou, S., Bareka, P. and Kamari, G. 2016. Karyomorphometric analysis of Fritillaria montana group in Greece. Comparative Cytogenetics, 10: 679-695.

Scheibe, B., Weiss, W., Bauer, C. and Gorg, A. 2001. Electrophoretic and immunochemical determination of bioindustrial enzymes in apple juice. European Food Research and Technology, 212: 691-695.

Sharma, S.C. and Maloo, S.R. 2009. Genetic diversity and phylogenetic relationship among soybean [Glycine max (L.) Merrill] varieties based on protein, evolutionary and morphological markers. Indian Journal of Plant Genetic Resources, 22(3): 260-266.

Sheidai, M. 2008. Comparative cytogenetic study of some grass genera of the subfamily Pooideae in Iran. Polish Botanical Journal, 53(1): 15-28.

Sheidai, M. and Moghadam, K.H. 2009. Cytogenetic studies in some species of Melica L. and Milium L. in Iran. Cytologia, 74: 185-193.

Sheidai, M., Attaei, S. and Khosravi-Reineh, M. 2006. Cytology of some Iranian stipa (Poaceae) species and populations. Acta Botanica Croatica, 65: 1-11.

Stace, C.A. and Aquier, P. 1978. Taxonomy and variation of Vulpia ciliatae Dumorr. Botanical Journal of the Linnean Society, 77: 107-112.

Stebbins, G.L. 1971. Chromosome Evolution in Higher Plants. Edwards Arnold Publisher Ltd., London, UK.

Sun, W., Ma, X., Zhang, J., Su, F., Zhang, Y. and Li, Z. 2017. Karyotypes of nineteen species of Asteraceae in the Hengduan mountains and adjacent regions. Plant Diversity, 39: 194-201. Sun, W., Wang, H., Wu, R., Sun, H. and Li, Z. 2020. Karyomorphological of three endemic plants (Brassicaceae: Euclidieae and Arabideae) from the Qinghai-Tibet Plateau and its significance. Plant Diversity, 42(3): 135-141.

Szczepaniak, M. and Cieślak, E. 2011. Genetic and morphological differentiation between Melica ciliata L. and M. transsilvanica Schur (Poaceae) in Europe reveals non-existence of M. ciliata in the Polish flora. Acta Societatis Botanicorum Poloniae, 80: 301-313.

Tehrani, M.S., Mardi, M., Sahebi, J., Catalán, P. and Díaz-Pérez, A. 2009. Genetic diversity and structure among Iranian tall fescue populations based on genomic-SSR and EST-SSR marker analysis. Plant Systematics and Evolution, 282: 57-70.

Tomaškin, J. and Tomaškinová, J. 2012. The ecological and environmental functions of grass ecosystems and their importance in the elimination of degradation processes in agricultural landscape. Carpathian Journal of Earth and Environmental Sciences, 7: 71-78.

Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M. and Webb, D.A. 1980. Ed. Flora Europaea, Volume 5, Alismataceae to Orchidaceae (monocotyledones). Cambridge University Press, Cambridge, England.

Tyler, T. 2002a. Geographic variation and dispersal history in Fennoscandian populations of two forest herbs. Plant Systematics and Evolution, 233: 47-64.

Tyler, T. 2002b. Large-scale geographic patterns of genetic variation in Melica nutans, a widespread Eurasian woodland grass. Plant Systematics and Evolution, 236: 73-87.

Zarif Ketabi, H., Shahmoradi, A.A., Dashti M., Paryab A., Hosseini Bamrood G.H. and Zarekia, S. 2010. Autecology of Melica persica Kunth. in Khorasan region. Iranian Journal of Range and Desert Research, 17(3): 421-430.